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Background: Cisplatin, a chemotherapeutic agent, is widely used in the treatment of malignant tumors. Nephrotoxicity, especially acute kidney injury (AKI), is the most common and severe adverse reaction of cisplatin. Resveratrol and ginsenoside Rg1, two natural products, have been found to have renal protective effects. However, the effects and the mechanisms in cisplatin-induced AKI need further investigation. Methods: The mouse models of cisplatin-induced AKI and several treatment groups were established. Male C57BL/6 mice were divided into five groups: saline control group, cisplatin injury group, resveratrol treatment group, Rg1 treatment group, resveratrol and Rg1 combined treatment group. Serological analysis of serum urea nitrogen was aimed to reflect the function of kidney, and histological analysis of renal tissue sections was aimed to assess the damage of proximal convoluted tubules. The expression levels of autophagy-related proteins Beclin 1 and LC3 were detected by western blotting and qRT-PCR respectively. Results: The renal function was improved and renal damage was alleviated in Rg1 and resveratrol alone or combined treatment groups compared with the cisplatin injury group. For the mechanism, treatment with Rg1 and resveratrol alone or in combination decreased the expressions of Beclin 1 both at protein and mRNA levels, decreased LC3II/I protein levels, indicating that autophagy was inhibited by treatment with Rg1 and resveratrol alone or in combination. Conclusion: Resveratrol and Rg1 alleviated the kidney damage caused by cisplatin, and reduced autophagy was involved in the renoprotective effects of resveratrol and Rg1 against cisplatin-induced AKI. This study may provide new evidence to alleviate cisplatin-induced AKI.

Background: Nephrotoxicity, especially acute kidney injury (AKI), is the main dose-limiting toxicity of cisplatin. Although recent studies showed that curcumin prevented cisplatin-induced AKI effectively, further studies to understand the mechanism are required. Methods: We established an AKI mouse model. Male C57BL/6 mice were assigned to three groups: saline group (control), cisplatin group (CP), and curcumin + cisplatin group (CP + Cur). The CP group received a single intraperitoneal (i.p.) injection of cisplatin, while the control group received saline. The CP + Cur group received i.p. curcumin three days before cisplatin injection and curcumin administered for another three days until the day before euthanization. Renal injury was assessed by serological and histological analysis. Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) were used to detect the phosphatase and tensin homolog (PTEN), and microRNA (miR)-181a expression in the renal tissues. Bioinformatics prediction and western blotting methods validated the targets of miR-181a in vitro. Results: Curcumin treatment alleviated cisplatin-induced nephrotoxicity as validated by the blood urea nitrogen (BUN) values, and histological analysis of kidneys. At the molecular level, curcumin treatment decreased miR-181a expression level, which was induced by cisplatin and restored the in vivo expression of PTEN, which was suppressed by cisplatin. We verified the direct regulation of PTEN by miR-181a in cultured human embryonic kidney 293T cells. Conclusions: We showed the involvement of miR-181a/PTEN axis in the renoprotective effect of curcumin against cisplatin-induced AKI, and provide new evidence on the ability of curcumin to alleviate cisplatin-induced nephrotoxicity.

This paper simulated the microvibration response of a micro-electronic workshop by using the finite element analysis software Sap2000. Based on the measured vibration signals as input data at the base, the displacement and velocity curves at some typical points on the work platform of a micro-electronic workshop under uniform and multipoint excitation were obtained. The results show that displacement responses at nodes of beams or in the direction of lower stiffness under multipoint excitation are much greater than those under uniform excitation, while there are no marked difference in the peak values of velocities.

The development and characteristic of agricultural sustainability indicators system (SI(subscript s)), at home and abroad, was reviewed. It was pointed out that the existing domestic methods of sustainability evaluation can not be accepted by foreign scholars, and their scientific basis is inadequate. The experience and shortcomings of the previous studies on the indicators selection and evaluation methods were analyzed, also with the significance for practice. Finally, a new SI(subscript s), for agriculture development evaluation was proposed, together with the corresponding calculation and evaluation methods. The farmer development index indicator was proposed to embody the viewpoint that the development of people is the ultimate aim; the dynamic weight calculation method was designed to reflect the various significance of the different indicators when their values were changing; and new indicators integrate methods were also explored.

CSCW (computer supported cooperative work) means a group of people cooperate to finish the same task with the help of computers. Under such a working environment, a safety mechanism is vital important to ensure unwanted information leakage. At present the encryption algorithms widely used in CSCW system are inefficient, and can't realize the real-time communication model satisfactorily. DES and ECC are both widely recognized data encryption algorithms, which have already been applied to CSCW security system, but each has his own strengths and weaknesses. DES is efficient but its key is easily revealed; the key of ECC is well managed, but the process efficiency is lower than DES. This paper puts forward a combined encryption algorithm based on 3DES and ECC to improve the performance of the CSCW system encryption. It is proved that the algorithm is feasible and effective.