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Soybean (Glycine max L. Merr) is a legume that contributes to food security and poverty reduction in Benin. Unfortunately, its culture is subject to several undocumented constraints that contribute to decline in productivity and a loss of diversity. Therefore, documentation of production constraints, varietal diversity seed system and traditional uses of soybeans in main production area of Benin Republic is necessary for its conservation and sustainable use. To fill these gaps, 57 villages were prospected in southern and central Benin using the tools and techniques of participatory research such as individual surveys using a questionnaire and field visits. A total 445 soybean producers were surveyed in the study area. The results show that soybean local nomenclature and local taxonomy were mainly based on seeds size. Thirty-five soybean local varieties were recorded corresponding to 7 varieties differentiated by morphological seed characteristics. The number of soybeans local varieties ranged from 1 to 4 per village. Farmers have abandoned some soybean local varieties and the main reasons for abandonment were agronomics. Eight varietal preference criteria were identified in the study area with high yield and seed size being important. The ravages of hares and rabbits were the main constraints to soybean production in both region. The soybean seed system was essentially informal. Various uses (food, feed and commercial) of soybeans, which underlie its conservation in traditional Beninese agriculture, have been recorded. As part of the development of conservation strategy of an existing soybean diversity in southern and central Benin, morphological and molecular characterization were recommended.

Adjahossou VN, Gbemavo DSJC, Abidja S, Laly J, Gbaguidi AA, Anagonou AD. 2021. Folk classification and traditional uses of Cyperus esculentus, a neglected and underutilized species in Benin. Biodiversitas 22: 2972-2979. The sweet pea or tigernut (Cyperus esculentus L.) has very high potential to be developed but is considered a neglected and underutilized plant in Benin. The current study aims to investigate the indigenous knowledge related to local diversity, production and uses of various landrace types of tigernut grown in its production areas in Benin. Using Participatory Research Appraisal (PRA) method, we surveyed 113 tigernut farmers in different regions of production in Atacora Department, Benin. Univariate statistics were performed to describe and compare tigernut farmers' responses on botanical and agronomic criteria. We found that the tigernut farmers in the studied area distinguished three landraces of C. esculentus. Two landrace types produced yellow-skinned tubers and the other produced black-skinned tubers. Economic value, agro-morphological traits and organoleptic quality were the criteria considered by the farmers when choosing the type of landrace to cultivate. According to the farmers, the attacks of the tubers by earthworms and termites constituted the main constraints of the production of tigernut. Local people used the species mainly for food, while small proportion was for medicine (e.g., for aphrodisiac) and soil fertilization purposes. This study implies that C. esculentus in Benin deserves better attention to be conserved and developed.

In northern Benin, several agroforestry species are nectar-producing, with high medicinal and food values. These species are currently threatened by the destruction of their habitats caused by deforestation and agriculture, mainly cotton production. Considering their importance for humans and beekeeping, this investigation was carried out in one of the most important honey production areas in Benin. Individual interviews and focus group discussions were conducted to identify the medicinal and food plants associated with honey production. Data were collected on food and medicinal uses, beekeepers' preferences in the choice of melliferous species and constraints faced for honey and medicinal species conservation in the areas studied. From these data, 46 species of medicinal and food species were identified as melliferous. Thus, nine new species were added to the list of melliferous species in Benin. Most of the species are also used as sources of human food or medicine in traditional set up. Species preferred by beekeepers were those that provide large amounts of sweet, fragrant hone. It appears that honey from 11 plant species with a high use value was clearly recognized by beekeepers who are involved in various human diseases' treatment. The constraints hampering the management of these melliferous species are extensive farming, animal husbandry, anarchic logging. This study brings us the avenues for modernization of beekeeping and the possibility of producing therapeutic honey.

Background Peanut ( Arachis hypogaea L.) is one of the major oilseed legumes contributing to food security and poverty reduction in Benin. Unfortunately, several constraints hamper peanut production in Benin leading to a low yield. Knowing that seed is crucial for increase agricultural productivity, a good knowledge of on-farm management of grown peanut diversity and uses that allow its maintenance in traditional Beninese agriculture are prerequisites to establish an efficient breeding and conservation programs. Therefore, this study aim to document peanut varietal diversity, folk nomenclature, seed system, storage constraints, seeds pest management practices, varietal preference criteria, cultural taboos and uses in southern and central Benin. Methods Two hundred and sixteen (216) farmers were surveyed through 32 villages in southern and central Benin using research tools and techniques of rural appraisal such as field visits, group surveys and individual surveys using a questionnaire. Results Fifty-four peanut folk varieties grouped into 8 morphotypes based on the seed characteristics were registered in the study area. All local names given to peanut folk varieties by ethnic groups have meaning. The number of folk varieties maintained per village ranged from 1 to 11 (an average of 4) and varied between 1 and 4 per household. Some peanut producers reported the disappearance of some folk varieties. The drop in yield was the main reason of peanut varietal disappearance in the study area. Rodent attacks were the most important constraints of peanut production in the study area. Varietal preference criteria varied from ethnic groups with seed size and many pod/seeds per plant as the main preferred traits. Peanut seed system was mainly informal. For peanut seed conservation, farmers use various storage tools. To alleviate pest problems in stored peanut, producers used plant biocides and synthetic chemicals. Some cultural taboos and various uses (food, feed and medicinal) of peanuts have been recorded in the southern and central Benin. Conclusions Our results suggest that for boost peanut production in central and southern Benin, breeders must create high yielding peanut varieties. For the adoption of these new varieties, it is important to take in account the preference varietal criteria of each ethnic group. To a good estimation of peanut diversity in the study area, morphological and molecular characterizations were recommended.

Corchorus olitorius L. is one of the traditional leafy vegetables of high food and socio-economic value in Africa. Although widely exploited, its varietal diversity is unknown and its domestication remains challenged by pests and diseases presently observed among subsistence users. This study aims to determine the morphotype diversity of C. olitorius in Southern Benin and the durability of its agroecosystem by the influence of agricultural practices on the major pest insects related to its production. Using participatory research methods, surveys were conducted in 21 villages randomly selected across the study area. Results revealed seven different morphotypes of C. olitorius, three of which were wild but used in traditional medicine and four were cultivated. Among the nine constraints reported by farmers, the attack of seven pest insects and the non-distinction of the seeds of the different cultivated morphotypes are the two most important constraints. The seven most common potential pest insects observed by between 10–100% of the farmers, in study area are: Acrea sp, Aulacophora Africana, Helicoverpa armigera, Spodoptera litoralis, Zonocerus variegatus, Podagrica spp. and Acrea acerata. The infestation by these insects were not linked to specific morphotypes of C. olitorius, but varied by locality. The agricultural practices that might influence the presence of any C. olitorius pests were intercropping and dormancy cutting, seedling after sowing and number of harvests. This information will contribute to a better production of the varieties of C. olitorius and the different crop associations could be integrated into methods of ecological management of the pests of C. olitorius.

Taro ( Colocasia esculenta L. Schott) and tannia (new cocoyam) ( Xanthosoma mafaffa L. Schott) are food crops cultivated in tropical and subtropical regions of Asia, Africa and Latin America. In cultivation, they are always vegetatively propagated and their production faces many constraints. Their corm is an important source of carbohydrates and leaves eaten as vegetable provide important minerals and vitamins. In Togo, although these crops are among the most important food crops in some rural areas, they appear at the country level as neglected and underutilized species (NUS) and should be promoted. However, adequate information related to production, constraints, farmers’ preferred traits and utilisation is lacking for the development of efficient breeding schemes and their sustainable preservation and use in Togo. In this paper, a focus group survey was conducted in all five ecological zones of the country to document farmers’ perceptions regarding constraints for production of these two crops and their preferred traits for production and utilization. Collected data were analysed through descriptive statistics (frequencies, percentages and means) using the Statistical Package for Social Scientists (SPSS 20) and Excel spreadsheets. Rainfall irregularity (or shortage of rainy seasons) (68.4% of responses), soil infertility (61.5% of responses) and inefficient marketing (58.7% of responses) were the most common production constraints reported. Farmers preferred early-maturity (73% of responses) and high-yield (65% of responses) as crop traits. Men are more involved in production of these crops in Togo (79% of respondents). However, the constraints and preferences reported were not gender dependent. This study showed that production of taro and tania faces many challenges in Togo. Morphological and molecular characterization of grown varieties is highly recommended to help select suitable varieties for breeding programmes. Improvements in food processing and accessibility of credit should be prioritized to generate more income for farmers and encourage production in Togo. Keywords: Agriculture, Colocasia esculenta , constraints, farmers, preferences, production, Togo, Xanthosoma mafaffa

Bambara groundnut (Vigna subterranea (L.) Verdc.) is one of the important legume crop grown in marginal soils of sub-Saharan Africa. Despite its importance in food security and income generation for small scale farmers, it remains as a neglected and underutilized crop and the productivity is very low in the field due to the lack of improved varieties and lack adequate farming practices. Thus, the aim of this study is to investigate all aspects related to its production including source of seeds supply and farmers management practices, utilization, conservation and marketing. The results revealed that in this Region of Western Niger Bambara groundnut was mainly produced by female (95%) compared to male (5%). Farmers use their own recycled seed (80%) which is the main source followed by provision of recycled seeds by relatives (5%) and purchasing from the local market (15%). Also, this finding shows that there is a lack of adequate farming practices and the crop is mostly produced on inherited land without any inputs as reported by 80% of the respondents. Mono-cropping is mainly practiced by 97% of farmers while a few of them do rotation (2%) and most of pre and postharvest handling technologies are traditional. The most important traits preferred by Bambara farmers is seed colour (cream color), followed by high grain yield, early maturity and cooking ability according to the respondents with 98, 92, 88 and 72% respectively. Several features have to be taking into account in order to promote Bambara nut including its ability to do well in harsh conditions, its nutritional benefits, and its ability to fix nitrogen, thereby increasing soil fertility in mixed cropping systems.

Background Common bean (Phaseolus vulgaris L.) is an important grain legume crop grown in the central region of the Republic of Benin. However, its production declined in recent years to the extent that its diversity is being threatened with extinction. Understanding the folk nomenclature and taxonomy, as well as use values that allow its maintenance in Beninese agricultural system, is a prerequisite to develop efficient strategies for its conservation. Knowing that each sociolinguistic group develop various uses and traditional knowledge for their crop genetic resources, we hypothesized that enhancement of farmers’ livelihood, thanks to the use values of common bean landraces, differ from one sociolinguistic group to another and contribute to their conservation in the traditional agriculture of central Benin. Methods Hundred and one common bean producers belonging to seven sociolinguistic groups selected through 23 villages of the region under study were surveyed. Data were collected through participatory research appraisal tools and techniques (individual interviews and direct observation) using a semi-structured questionnaire. Folk nomenclature and taxonomy of common bean, local uses, and factors affecting them were investigated. Results Across the seven sociolinguistic groups surveyed in the study area, five common bean generic names and 26 folk varieties corresponding to 12 landraces have been recorded. Folk nomenclature and taxonomy were mainly based on seeds’ coat color. The present study has revealed five common bean use values in the study area (food, medicinal, commercial, fodder, and mystic-religious), which are influenced by sociolinguistic groups. Leaves, roots, and seeds of three common bean folk varieties are used by surveyed farmers for disease treatment. Nine common bean folk varieties are considered by farmers as magical plants which have supernatural properties while several taboos for deities’ followers regarding Séssé landrace are inventoried across sociolinguistic groups. Level of education and age of respondents influence positively and significantly medicine and mystical-religious uses of common bean respectively while commercial use is positively influenced by age and negatively by gender. Conclusions Traditional values associated with common bean landraces in the central region of the Republic of Benin increase their chance of survival in the farming systems. Socio-demographic characteristics of respondents which influence common bean use values must be taken into account in future programs of conservation. However, an assessment of diversity and analysis of distribution of extend of common bean landraces in the study area is a necessity for the development of an efficient strategy of conservation of this genetic resource.

Bambara groundnut (Vigna subterranea (L.) Verdc.) is a neglected and less exploited species in Benin. However, the rational use of the different landraces available involves an appropriate knowledge of their characteristics. This study examined the characteristics of 52 local landraces of Bambara groundnut traditionally cultivated in Benin based on 12 qualitative and 11 quantitative variables, in order to expose those of good qualities. After a classification based on the morphological variables of the seeds, the experimental field was installed in a completely randomized device of blocks repeated three times. The collected data was analyzed with Statistica 7.1 and NTSYS PC 2.2. softwares using qualitative characteristics (Growth’s type, Stem’s hairiness, Flower’s colour, Leave’s shape etc.). As result, the number of morphotypes increased from 18 (out of classification) to 47. Therefore, it appears that the Benin collection of Bambara groundnut is broad in morphological variability. The analysis of the principal component of the quantitative variables revealed three specific classes within the collection. The most efficient variety which belonged to the second class, had a high field yield per hectare (> 1 t/ha). The Souhouloumankpa variety yielded the earliest (103 days after sowing) in addition to the high field yield. The study also showed a positive relationship between 100 seed’s weight, their length, the number of seeds per plant and the seed’s field yield in kg/ha. For a sustainable preservation and an improvement of the available resources, a molecular characterization of the Bambara groundnut landraces in Benin Republic needs to be done.

Background: Although termites are considered as agricultural pests, they play an important role in maintaining the ecosystem. Therefore, it matters to investigate the farmers' perception of the impacts of the termites on the agriculture and their indigenous utilization. Methods: A semi-structured questionnaire was used to interview 94 farmers through 10 villages of Atacora department, in the northwestern region of Benin, to obtain information for the development of successful strategies of termite management and conservation. Their perceptions on the importance and management of termites along with the indigenous nomenclature and utilization of termite mounds were assessed. Termite species identified by farmers were collected and preserved in 80% alcohol for identification. Results: Eight crops were identified by farmers as susceptible to termites with maize, sorghum, and yam as being the most susceptible. According to farmers, the susceptibility to termites of these crops is due to their high-water content and sweet taste. A total of 27 vernacular names of termites were recorded corresponding to 10 species, Amitermes evuncifer, Macrotermes subhyalinus, and Trinervitermes oeconomus being the most damaging termite species. All the names given to termite species had a meaning. The drought was identified by farmers as the main factor favouring termite attacks. Demolition of termite mounds in the fields was the most commonly reported control method. Salt and other pesticides were commonly used by farmers to protect stored farm products. The lack of effective control methods is the main constraint for termite management. In northwestern Benin, farmers reported different purpose utilizations of termite mounds and termites. Conclusions: The study has shown that farmers perceived termites as pests of several agricultural crops and apply various indigenous control practices whose efficiency need to be verified. Utilization of termites and termite mound soil as food and medicinal resources underlines the need for a more focused approach to termite control for the conservation of non-pest termite species. The sensitization of farmers on the importance of termites as well as the development of an integrated control method to combat termite pests proved necessary., SCOPUS: ar.j, info:eu-repo/semantics/published

Cassava (Manihot esculenta Crantz) is one of the highly produced and consumed food crops in Benin Republic. Through the application of participatory research appraisal tools and techniques, such as direct observations, group discussions and field visit, cassava production constraints, the diversity at the community level and farmers’ varieties preference criteria were assessed in southern Benin. Base on national statistic cassava production fifty five (55) villages were selected and surveyed in the southern Benin. Descriptive statistics were used to analyse the data collected. About 11 constraints were listed by farmers, and the most important were low productivity, low post-maturity underground storage capacity of the roots of some cultivars, viral disease (cassava mosaic disease), susceptibility to high soil moisture, and lack of early maturing cultivars, which together accounted for 68.02 % of the total responses. Additionally, a total of 125 existing landraces were recorded. The distribution and extent pattern revealed 59 elites cultivars (cultivated by many households and on large areas) that were further grouped into 23 categories based on their agronomic, technological and culinary characteristics. In most of the villages, the farmers’ perception of cultivar loss was based on abandonment of some cultivars due to their poor agronomic (69.12 % of responses) and culinary and/or technological attributes (30.88 % of responses). Within the existing diversity, cultivar choice or preferences are based on 22 culinary, technological, agronomic and economic criteria and their importance vary across the different department. Among these criteria, the most important were high productivity, good quality of the gari, early maturity and good friability accounted together for 48.12 % of the total responses. The presence of duplicates or synonyms was also observed and it is suggested that both morphological and molecular characterization and classification be carried out for better identification of cultivars.

Variability study of agro-morphological characteristics is important for varietal improvement and plant breeding program. For this, 148 chili pepper landraces (Capsicum annuum L.) collected from Northern Benin were characterized using 39 (21 qualitative and 18 quantitative) morphological characters. The trial was conducted in a Fisher block design with 3 replicates at the experimental station of the Faculty of Science and Technology (FAST) of Dassa based in Central Benin. The results revealed considerable morphological variability between landraces. Twenty nine (16 quantitative and 13 qualitative) variables among the 39 examined were discriminatory and group the landraces into 4 classes when considering the qualitative variables. With quantitative variables, 9 groups were obtained. The correlations between the studied quantitative variables show that the yield parameters are correlated with the variables related to the vegetative part of the plant. This study will help breeders to better select their parents for crossing in order to produce desirable and high yielding varieties.

Cassava (Manihot esculenta Crantz) is an important food security crop or resource for poor rural communities particularly in Africa. The crop’s ability to produce high yields even under poor conditions and storability of its roots underground for longer periods or until needed makes it a model ‘food security crop’. In Benin Republic, cassava has been recognized as one of the major crop contributing towards dynamic value chains generating incomes for small-holder farmers. The crop is grown all over the country, however, the increased production are mainly recorded from far south and central parts of the country. Genetic improvement of cassava in Benin Republic is limited because of poor knowledge of genetic diversity present within the country. The main objective of this study was to assess the genetic diversity and relationships among elite cassava varieties collected from different regions of Benin using fluorescently labelled simple sequence repeat (SSR) markers and to compare the results with farmer’s knowledge and morphological traits. A total of 96 cultivars collected from major cassava growing areas such as Southern and Central Benin were classified into 24 different groups using farmers’ knowledge, while classification based on 18 morphological traits resulted in five groups. In total, sixteen SSR markers were tested for molecular analysis of the ninety-six cassava varieties. Among the sixteen, twelve SSR markers gave good banding pattern and were used to genotype the varieties. An average of 3.58 and 0.47 for number of alleles and polymorphism information content respectively was observed. The observed heterozygosity (Ho) ranged from 0.23 to 1.0 with an average of 0.66 indicating moderate level of diversity among the cultivars. Based on the proportion of shared alleles and hierarchical clustering, the 96 elite cassava varieties were classified as 74 unique varieties. Principal component analysis and analysis of molecular variance revealed no significant variation between the regions thus, explaining regular exchange of planting materials among cassava farmers across various regions. The moderate level of genetic diversity in famer’s field, revealed in the present study, is a good indication of the need for broadening the genetic base of cassava in Benin Republic and establishing a formal breeding program in the country.

Kersting’s groundnut (Macrotyloma geocarpum) locally named “Doyi” is a grain legume of high nutritional and economic value widely consumed in Benin but neglected by the scientific research and the development programmes. In order to document the indigenous knowledge related to the production, diversity and use of this crop and to collect samples for characterisation and evaluation, 30 villages from South and Centre of Benin were randomly selected and surveyed. In each village, 10–15 producing households (374 farmers in total) were chosen as above and interviewed using participatory research appraisal tools and techniques. The study revealed that the production of Kersting’s groundnut is particularly concentrated in central Benin (departments of Zou and Collines), but is seriously declining for ten reasons. As reported by farmers, the most important factors of those included the high cost of production (40.7 % of responses), the susceptibility of cultivars to high soil moisture (25.5 % of responses) and the complexity of the cultural practices (25.2 % of responses). Only three local cultivars differing mostly by the colour of seed coat (white, red and black) were recorded. The white seeded cultivar was the most cultivated and preferred for consumption. Gender role analysis indicated that young women were the most involved in the cultivation of M. geocarpum. Phenotypical analysis of the different accessions (32 in total) collected during the study revealed only three groups that differ mainly in leaf width, yield and date to 50 % flowering. Agronomic evaluation indicated a significant difference (p

Sweet potato is an important staple food in Benin, which unfortunately remains neglected by research and underutilized. To document the diversity of the crops for its sustainable conservation and use, an ethnobotanical investigation was conducted in 34 villages selected in Central and Southern Benin using participatory research approach methods and tools. For 24.59 % of the respondents, sweet potato is ranked as the sixth most important crop produced. 19.36 % of the respondents produced sweet potato for feeding purpose only, while 46.77 % of respondents are growing it for purely economic purposes. At total 12 constraints related to the sweet potato production were registered through the study area, among which eight, which represent 66.67 % of the constraints could be solve by the scientific research efforts while the rest (four constraints, 33.33 %) were purely of political order. The difficulty of farming practices (20.39 % of responses) and the sensitivity of the crop to the attack of pests and insects (19.74 % of responses) appeared as the major constraints cited by the respondents. The numbers of sweet potato varieties per village range from 2 (two) to 11 (eleven) with an average of 5 varieties per village. The Shannon diversity index of the South (4.06) is greater than the one of the Centre (4.00) and shows that South region has a relatively highest range of varieties and so suitable for the crops diversity conservation. At 65 % of similarity, the cluster analysis using UPGMA dendrogram classified the 108 sweet potato cultivars identified in eight groups corresponding to 92 morphological units based on 13 agronomic traits and culinary characteristic. Within the varietal group, it denotes some yellow (27.45 %) and orange (3.92 %) flesh variety groups of sweet potato which constitute potential source of vitamin A. The study of the vitamin A (caroten) content of those varieties is to be considered to fully exploit their nutritional potential.

Jute mallow rich in proteins, vitamins and essential amino acids is an important leafy vegetable in Africa. Ethno-botanical knowledge on the crop in Ghana has been only incompletely documented and little is known about variation and diversity of local accessions. In order to document available ethnobotanical knowledge and investigate diversity of local accessions, 4000 farmers in twenty districts were surveyed using participatory rapid appraisal tools and techniques. 50 accessions of local germplasm were collected from these districts. Using randomized complete block design with three replications, the 50 accessions collected were characterized based on 12 quantitative and 6 qualitative traits. Results of the survey recorded several vernacular names of the crop and their meaning referring to the slimy nature of the leaves. Some local cultivars which were known in the past are no longer cultivated in farmers’ field suggesting genetic erosion of jute mallow in Ghana. Apart from food, respondents use jute mallow to treat fever, waist pain, stomach problems and loss of appetite indicating nutraceutical potential of the crop. Hierarchical cluster analysis grouped the accessions into four distinct clusters and individuals from the same geographical origin were separately classified. Quantitative traits such as leaf length, number of branches per plant, and number of leaves/plant defined the first principal component. Accessions Cagric 26, Cagric 28, Cagric 41, Cagric 08 and Cagric 01 recorded high yields. These accessions could serve as parents for breeding of improved cultivars.

Guinea yam (Dioscorea cayenensis–D. rotundata complex) is an important tuber crop that highly contributes to food security and poverty alleviation in Benin. The knowledge and understanding of the extent of genetic variation of Guinea yam germplasm is important for planning of the genetic conservation, and the utilisation of this resource. The objective of this study was to assess the genetic diversity and relationships of 64 yam landraces whose dried chips are considered as resistant to insect attacks using 41 simple sequence repeat. Among these primers, 13 were found to be polymorphic, giving 113 polymorphic alleles. The number of alleles per locus ranged from 4 (Ym50) to 13 (Ym29), with an average of 8.69. Unique allele was observed with some landraces (Singou and Tchakatchaka) and can be considered as unique gene and use in yam breeding program. The mean polymorphic information content values for all markers used was 0.76 and ranged between 0.58 and 0.91 in loci YM3 and YM32 respectively. The genetic distance of yam landraces ranged from 0.45 (Yasoubagarou) to 0.04 (Assinapeira and Alahina), indicating that the yam germplasm has a high degree of genetic diversity supported by an averagely observed heterozygosity of 0.78. Cluster analysis using unweighted pair group method with arithmetic average grouped the 64 yam landraces into two distinct clusters. This tendency was also observed in the principal coordinate analysis. The analysis of molecular variance revealed that 96 % of the variation was found within the population and only 4 % between the populations. Genetic diversity and relationship assessments among the 64 yam landraces of Benin could provide useful information for efficient use of these materials, especially for genetic improvement.

Chili peppers play a significant role in the world diet and can contribute to the improvement of horticultural producers’ incomes. In Benin, chili pepper production is hampered by many challenges that need to be characterized, prioritized and addressed. Moreover, the existing diversity, as well as the agronomic potential, of the varieties being cultivated in Benin is still not well understood. In order to define the scientific basis for the improvement of chili pepper production and the preservation of its diversity, one hundred villages were randomly selected and surveyed in Northern and Central Benin by using participatory research appraisal tools and techniques. Twelve production constraints were identified, of which low productivity (19 %), soil infertility (18.5 %), drought (18.3 %), pests and disease susceptibility (17.9 %), early fall of plant organs (15 %) appear as the most important. In terms of diversity, 77 chili pepper varieties (40 from the frutescens group, 24 from the annuum group and 13 from the chinense group) including 72 local and five introduced varieties (Tatache, Yeyekouka, Yeyekouokoure, Cote d’ivoire and MC) were found. The number of varieties varies from three to seven (four on average) per village and from one to five by household. The proportion of loss of diversity per village varies from 0 to 75 % with an average of 26.61 %. Susceptibility to pests and diseases (37.1 % of responses), early fall of plant organs (11.6 % of responses) and susceptibility to drought (8.5 %), were the most important reasons given in attempts to explain the loss of diversity. Farmers’ varietal preference criteria are essentially agronomic (90.4 % of responses). The participative agronomic evaluation revealed that the varieties of frutescens group are significantly higher with respect to the evaluated variables, but they are less appreciated by the consumers.

Neglected and underutilized crop species (NUS) are important genetic resources for nutrition, food security, medicine, management of agricultural risk and income generation for rural communities in Ghana. Little is known about their diversity, farmers’ management practices and safety of NUS conserved on-farm while these are prerequisites for developing strategic action plans to ensure their conservation and thus their continued utilization. This study was designed to (1) assess the diversity, forms of consumption and management practices of NUS in Ghana, (2) investigate constraints of on-farm conservation of NUS and the linkage between cultural practices in traditional farming systems and the safety of the NUS conserved on-farm in Ghana. A survey involving 1800 respondents was carried out in nine (9) farming villages of Ghana using participatory research appraisal tools. Seventy-two NUS species were identified on farmers’ field. The grouping of these species into major commodity crops suggests high diversity of NUS genetic resources in Ghana which could be used to further enhance nutrition and food security. The high market value of some of the NUS species suggest that they are well known and consumed. Mass use of systemic weedicides by farmers, activities of herdsmen and lack of knowledge on conservation of seeds and tubers are the main factors reported by farmers as the cause of genetic erosion of NUS on their farms. These findings create an urgency to collect and conserve genetic resources of NUS in Ghana to promote their utilization and breeding of improved varieties.

Sorghum (Sorghum bicolor (L.) Moench) is an important staple food in semi-arid tropics which contribute to food security and poverty alleviation in Benin. However, its production is seriously facing enormous abiotic and biotic stresses including climate variability. To document its cultivar diversity, the impact of climate change on its production and diversity, the adaptation strategies developed by farmers and the performance of landraces, 22 villages were randomly selected and surveyed in the Department of Atakora in the northwest of Benin using participatory research appraisals (fields and granaries visit, individual and group survey using questionnaires, Four cells or distribution and extent analysis, etc.). Data were analysed through descriptive statistics (frequencies, percentages, means, etc.) to generate summaries and tables at different (villages, individuals) levels and also through multivariate analysis (cluster analysis) and the results figures. In total 8 constraints affecting sorghum production were recorded among which striga proliferation, soil poverty and climate change effects (drought; excess of rain; delay, insufficient and irregular rainfall, etc.) were the most important. Subject to synonyms, 89 sorghum landraces were inventoried and their number varied from 4 to 17 (7 on average) per village. The Shannon–Weaver diversity index (H) estimated at 3.02 indicated high sorghum diversity in the study area. Many landraces were threatened. The relatively high rates (16.7–88.2 %; 40.9 % on average) of cultivars loss recorded per village are proofs of menace of genetic erosion and necessity of developing conservation strategies. Inadaptability of cultivars to climate variability (39.6 % of abandoned landraces) was among others the most important reason justifying the loss of diversity. Crop rotations, growing of early maturing and/or drought tolerant landraces, establishment of fields in lowlands are the most important strategies developed by farmers to mitigate impacts of climate change (low productivity, increase of damages from storage insects, early drying of plant leaves etc.) on the crop. Participatory evaluation of the existing landraces led to the identification of some high-performing ones that are resistant/tolerant to diverse abiotic and biotic stresses. Farmers’ cultivars preference criteria were identified and prioritized. Results of this study are useful to policy makers, agricultural extension services of the different districts, genetic resources specialists and breeders in order to improve sorghum production in Benin.

A study was conducted in five yam-growing communities in the Forest Transition Agroecological Zone of Ghana to identify cultivated yam varieties, their distribution and intensity of cultivation and to document the rate and causes of landrace germplasm loss. Generally, the cultivated varieties ranged from 9 to 16 with a mean of 12. The most diversity was found in Sankore (Asunafo South District), followed by Sampa in the Jaman North District, Asantekwa in the Kintampo District, Ejura in the Ejura-Sekyedumase and Mim in the Atebubu/Amantin Districts with 16, 15, 12, 11 and 9 cultivated varieties respectively. Dioscorea rotundata is most widely cultivated species of yam followed by Dioscorea alata, Dioscorea cayenensis, Dioscorea praehensilis and Dioscorea bulbifera respectively. Factors such as good culinary characteristics, high yield, seed generation capacity, good storage characteristics and resistance to biotic and abiotic stresses were important criteria for selection of variety. The market and utilization were major determinants of continual cultivation of a variety or its neglect. Key words: Agro-morphological, cultural, environmental, Ghana, technological.

Guinea yam (Dioscorea cayenensis Lam.–D. rotundata Poir. complex) is an important tuber crop that highly contributes to food security and poverty alleviation in Benin. However, its production is seriously affected by many biotic and abiotic constraints which could be overcame by concentrating the production on resistant or tolerant cultivars. In order to identify such cultivars that are known to exist in Benin traditional agriculture, 51 villages were randomly selected throughout the production zones and surveyed. Out of the 426 cultivars (subject to synonymy) evaluated using participatory approach and 13 agronomic, culinary, and technological traits, 25, 47, 49, 64 and 64 cultivars respectively tolerant to poor soils, nematodes, high soil moisture (adaptability to lowland), drought and chips (peeled and dried tuber) storage insects were, among others, identified. These pools of cultivars could serve in breeding program or be directly used in varietal exchanges towards the zones of interests. The agromorphological characterisation conducted per pool of performing cultivars revealed the existence of many duplicates that should be clarified with the use of molecular markers. Flowering, sex and intensity of flowering of the different cultivars were analysed and revealed a low rate (11.68 %) of non-floriferous plants, a high rate (61.03 %) of plants with low intensity of flowering and five types of male inflorescences indicating a hypothetical evolution towards the suppression of flowering with the cultivated yams.

In Benin, the shea sector is one of the most promoted sectors in government’s attempt to diversify sources of farm income. The objective of this study is to evaluate the impact of the adoption of the semi-mechanized shea processing on the income of rural women in northern Benin. In total, a random sample of 200 women processing shea was investigated. A probit model was firstly run to analyze the factors determining the adoption of the semi-mechanization. Then, a multiple regression model was used to assess the impact of adoption on women's income. The results highlighted that, the adoption of semi-mechanization was determined by availability of electricity, availability of a market, contact with extension services and shea processing as main activity for woman. Moreover, the adoption of semi-mechanization has induced a positive and significant increase of rural women’s income up to 103 914 Francs CFA. As a result, it is important that agricultural policy reinforce the promotion of modern agricultural processing technologies to improve the added value and to reduce poverty in rural areas. As well, electricity and market access, women’s level of education and contact with extension need to be improved. Key words: Adoption, impact, shea processing, semi-mechanization, income, Benin.

Objective: To investigate the expression and correlation of transforming growth factor-β1 (TGF-β1) and fibroblast growth factor receptor 4 (FGFR4) in human hepatocellular carcinoma (HCC) and the relationship with clinicopathological features and prognosis. Materials and methods: The expression of TGF-β1 and FGFR4 in 126 HCC samples was detected immunohistochemically. Combined with clinical postoperative follow-up data, the expression of TGF-β1 and FGFR4 in HCC and the relationship with the prognosis of patients were analyzed by statistically. Results: The positive expression rate of TGF-β1 was 84.1% (106/126) in tumors, and that in peritumoral liver tissues was 64.3% (81/126); the positive expression rate of FGFR4 in tumors was 74.6% (94/126) and that in peritumoral liver tissues was 57.1% (72/126). The expression of TGF-β1 and FGFR4 in the carcinoma tissues was significantly higher than that in peritumoral liver tissues (p < 0.05). Intratumoral TGF-β1 and FGFR4 expression was associated with TNM stage (p < 0.05). TGF-β1 and FGFR4 expression levels didn't significantly correlate with other clinicopathological parameters, including age, sex, tumor size, serum AFP level, tumor differentiation, lymph node metastasis, etc. (p > 0.05). TGF-β1 expression was positively correlated with FGFR4 expression (r = 0.595, p < 0.05). Patients with positive FGFR4 or TGF-β1 expression had shorter overall survival compared with negative expression (p < 0.05). Conclusions: The expression of TGF-β1 and FGFR4 could make synergy on the occurrence and progression of HCC, and may be used as prognosis indicators for HCC patients.

Thirty five villages randomly selected across ethnic and agro-ecological zones of Benin were surveyed to document the indigenous knowledge associated with the domestication (bringing into cultivation), production and utilization of vegetable Gbolo (Crassocephalum spp.). The study revealed the existence of different morphotypes (1–4 per village) corresponding to two botanical species (Crassocephalum rubens (Juss. ex Jacq.) S. Moore) and (Crassocephalum crepidioides (Benth.) S. Moore) found unequally distributed throughout the country. The domestication (bringing into cultivation from the wild) of Gbolo was started in many villages and with some tribes in southern Benin, the vegetable is already being produced in sole cropping for commercial purposes. Regular consumption (49.90 % of responses) and scarcity (20.30 % of responses) were the two main reasons attributed by the interviewees for domestication of the vegetable. The cultural practices recorded were of five categories. The commonest cropping system found was mixed or inter-row cropping (92.31 % of producers) with either vegetable crops (53.85 % of producers) or non-vegetable crops (38.46 % of users). Sauce prepared with either fresh or dried leaves was the main type of preparation of Gbolo and the vegetable was reported to possess special nutritional and medicinal values. For further promotion, important researches directions are suggested.

Sesamum radiatum is a nutraceutical traditional leafy vegetable highly consumed in Benin. In spite of its importance for food security, nutrition and income generation, very little is known about in the scientific community and it falls into the group of so-called neglected and underutilised species. In order to document the diversity and the various traditional cultural practices associated with the production of this species and assess its “bringing into cultivation” levels across zones, a survey was conducted in 10 villages randomly selected from different agroecological and ethnic zones of both northern and central Benin. Data were collected in the different sites through application of Participatory Research Appraisal tools and techniques and analysed using both simple descriptive statistics and multivariate analysis. The study revealed a rich folk nomenclature essentially centred on the slimy texture of the sauce. No apparent intraspecific diversity was reported within S. radiatum at village level but the agromorphological characterisation of 18 accessions collected from different regions of the country revealed the existence of clear and well-structured intraspecific diversity exploitable in breeding for the development of new varieties with the potential to attract premium prices in local markets. In most of the households surveyed S. radiatum was found at the steps 1–4 in the “bringing into cultivation process”. The production of S. radiatum is still traditional and biologic (no fertilisers, no pesticides). The cultural practices used vary with the producers and are grouped into four categories. Further agricultural experiments are needed to identify appropriate cultural practices for the large production of this species for food, nutrition and income generation. Strengthening the existing germplasm of this species with additional collection from more agroecological zones is proposed for thorough genetic characterisation.

Cassava (Manihot esculenta Crantz) is one of the most produced and consumed food crops in the Republic of Congo. To assess the diversity and understand the traditional management of its cultivars in the department of Bouenza, twenty-one villages randomly selected from nine ethnic zones were surveyed using participatory rural appraisal. Altogether, 86 land races cultivars were recorded and further grouped into 36 types based on their agronomic and culinary characteristics. The number of cultivars accessed varied from 6 to 21 per village (13 on average) and from 1 to 11 (4 on average) per household. Their distribution and extent analysis revealed some worrying rates of cultivar loss in the range of 7.41–66.67% (32.06% on average) per village. The reasons of cultivars abandonment, the folk nomenclature and the traditional cutting supply system and planting techniques were documented and their importance in terms of genetic resources conservation and utilisation are discussed. Twenty farmers’ cultivar preference criteria were identified and prioritised. Their relative importances were examined across ethnic groups. Among them, the most important were yield, taste, cossette quality and root size which account for 72.39% of the total responses. The study revealed that most of the farmers did not consider the importance of the sexual reproduction in the cultivar diversification and the impact of cassava mosaic disease on the productivity of the infested plants. Awareness efforts and extension of the study to other cassava production zones were recommended for food security needs in Congo. To clarify synonym, morphological classification and molecular analysis were also suggested.

Fonio (Digitaria exilis (Kipp.) Stapf, white fonio; Digitaria iburua Stapf, black fonio), is one of the oldest native, but neglected, cereal crops of cultural, nutritional and economic importance of West Africa. To gather key information that will help to preserve its diversity in Benin and develop modern and well-adapted varieties that could meet both farmers’ and consumers’ needs, a participatory research appraisal survey was conducted in 15 villages randomly selected in the fonio production zone of Benin. Altogether 15 farmer-named landraces were recorded and further grouped into five morphotypes of which four belong to D. exilis and one to D. iburua. The number of landraces inventoried varied from one to five per village (three on average) and from one to three per household. Their distribution and extent are presented and their importance in terms of genetic resources conservation is discussed. Farmers’ preferences for fonio varieties were assessed and eight preference criteria (earliness, culinary characteristics, ease of processing, productivity, facility of harvesting, grains size, storability, and drought tolerance) were identified and prioritised according to the gender. The traditional seed supply system and on-farm seed maintenance were investigated. Two plant species (Digitaria horizontalis Willd. and Digitaria longiflora (Retz.) Pers.) were reported by farmers as putative parents of cultivated fonio. Of these, only Digitaria longiflora has interesting agronomic traits (erect habit; stem long, big, strong and resistant to lodging; panicle long and rich in grains; large sized seeds) that are useful for the improvement of cultivated fonio. In the entire study area, the crop is under threat. Therefore, concerted efforts should be deployed to preserve its diversity and increase its use to combat food insecurity and malnutrition in Benin.

Seventy-three villages randomly selected in 29 ethnic areas and three regions or agro-ecological zones of Benin were investigated by a participatory rural appraisal survey to assess the diversity of the species used as traditional leafy vegetables (TLVs). In total, 187 plant species belonging to 141 genera and 52 families were recorded. Among these, 47 (25.13%) were cultivated and 140 (74.87%) were gathered from the wild. Herbs (64.78%) were the most numerous followed by shrubs (19.78%) and trees (15.50%). The Shannon-Weaver diversity index calculated was 3.232. The total number of TLVs used highly varies, across ethnic groups, from 15 (Toli tribe) to 58 (people Mahi) with, on average, 36 species per ethnic group. The relative proportions of the wild and cultivated species used also vary with the tribes but on average appeared almost the same. Of the species inventoried, 18 of national importance were found among which Solanum macrocarpon, Corchorus olitorius, Amaranthus cruentus and Gymnanthemum amygdalinum ranked first. The matrix scoring technique yielded 12 criteria of different natures used to define preference. Among these, four (taste, ease of preparation, availability and quantity of required condiments) were the most important and represent, all together, more than 72% of responses. Despite the diversity of species used as leafy vegetables, they were all basically consumed in the same way. Sauces were the main type of preparation and involved all the species. The perceived nutritional and medicinal (curative, regulative and stimulative) properties of the species as well as their cultural significance were documented. Some TLVs are known and consumed by all or many ethnic groups while many others were simply ethnospecific or used by only a few peoples. However, no correlation was found between the distribution of the utilisation of the species and their degree of consumption which all depend on the eating habits of the peoples. Cluster analysis revealed that peoples sharing a common geographical space and/or cultural identity or origin seem to consume almost the same types of TLVs and cluster together. Given the large quantity of evidence of the importance of the TLVs, there should be a systematic effort to improve their understanding and their uses to reduce if not alleviate rural poverty and malnutrition in Benin.

Since the Convention on Biological Diversity, the evaluation of varietal richness and its inherent management practices have become prerequisite tasks for in situ conservation. The participative method we are implementing in Sori village (Benin) on cowpea (Vigna unguiculata L) is consistant with such expectations. Basing the estimation of landrace richness on their local names, the participative approach used in this study made it possible to inventory 14 landraces in Sori. Ten mega-practices contributing to maintaining and/or enhancing the pool of landraces were inventoried, described and evaluated according to criteria such as the diversity of use, the optimization of farm resources and anti-risk strategies. For each practice, an aptitude value for conservation was calculated. These approaches revealed that botanic treatments, seed multiplication and cultural association are the most important practices according to value markers of agricultural practices. This study made it possible to test the use of particular a methodology in evaluating peasant management practices of agrobiodiversity. It also highlighted how local varieties are taken into account in the production systems and the agricultural policies of biodiversity conservation.

Cassava is one of the staple food crops contributing significantly to food and nutrition security in Benin. This study aimed to assess the diversity of the elite cassava cultivars of Bantè district, determine the physicochemical properties of the most preferred ones as well as the sensory attributes of their major derived products (gari and tapioca), and compare them with the farmers’ and processors’ perception on their technological qualities. The ethnobotanical investigation revealed existence of 40 cultivars including 9 elites that were further classified into three groups based on agronomics and technological and culinary properties. Clustered together, cultivarsIdilèrou, Monlèkangan,andOdohoungbocharacterized by low fiber content, high yield of gari and tapioca, and good in-ground postmaturity storage were the most preferred ones. Their physicochemical analysis revealed good rate of dry matters (39.8% to 41.13%), starch (24.47% to 25.5%) and total sugars (39.46% to 41.13%), low fiber (0.80% to 1.02%), and cyanide (50 mg/kg) contents. The sensory analysis of their gari and tapioca revealed very well appreciated (taste, color, and texture) products by the consumers. The confirmation by scientific analysis of the farmers’ perception on qualities of the most preferred cultivars indicated that they have good knowledge of their materials.

Guinea yams (Dioscorea cayenensis-rotundata complex; D. rotundata Poir. and D. cayenensis Lam.) have been described as resulting from a process of domestication of wild yams of the section Enantiophyllum by African farmers. Although currently practised by few farmers, the process of yam domestication is still on-going in Benin. In order to document the practices used and the indigenous knowledge maintained by Nago and Fon farmers, 27 villages were surveyed. In total, 57 farmers domesticating yam were identified, and 68 yams newly domesticated (or in domestication) were collected. Fon and Nago farmers domesticate yam mainly to widen the genetic basis of the existing diversity or for simple curiosity. Among the three wild yams species (D. abyssinica Hochst. ex Kunth, D. praehensilis Benth. and D. burkilliana J. Miege) used, D. praehensilis is the most important and the most exploited. Tuber of the wild yams are collected either in the bush (most often near the village) or in the forests (far from the village) during hunting. The domestication process consists of bringing into cultivation selected individuals which go through intense vegetative multiplication and selection procedures (over a lengthy but variable period of time) that induce morphological and biochemical changes in the plant mainly at the tuber level. Individuals resulting from these manipulations were found to be, either similar or identical to known landraces or completely new based on both morphological and isozyme analysis. Because it leads to some new cultivars, this process of domestication has potential in yam breeding and appears to be a strategy that could be useful to breeders, while developing a methodology for participatory breeding of yam.

Sesamum radiatum (Schum. and Thonn) is a traditional leafy vegetable of national importance in Benin. Although, it is cultivated and consumed in many regions of the country, it is still unfortunately neglected by scientific research. In order to fill in the knowledge gaps necessary for its varietal improvement, 16 accessions collected from different agro-ecological zones were characterized using 16 quantitative morphological traits. A significant variability was found among the accessions. Day to flowering, day to fruiting, total biomass, plant height, noose diameter, number of fruits per plant and number of seeds per fruit were some of the most discriminating parameters. Multivariate analysis (principal component analysis and cluster analysis) revealed four distinct agro-morphological groups of varying performance which will be highly useful in varietal improvement. Correlation analyses between descriptors revealed uniquely desired positive and negative relationships hence, indicating predictable success for eventual breeding program. To further investigate the genetic basis of the phenotypic diversity revealed among S. radiatum accessions, the use of molecular markers such as amplified fragment length polymorphisms (AFLPs) is recommended. (Resume d'auteur)

All over the world, plant domestication is continually being carried out by local communities to support their needs for food, fibre, medicine, building materials, etc. Using participatory rapid appraisal approach, 150 households were surveyed in 5 villages selected in five ethnic groups of Benin, to investigate the local communities’ motivations for plant domestication and the contributions of this process toin situconservation of genetic resources. The results indicated differences in plant domestication between agroecological zones and among ethnic groups. People in the humid zones give priority to herbs mainly for their leaves while those in dry area prefer trees mostly for their fruits. Local communities were motivated to undertake plant domestication for foods (80% of respondents), medicinal use (40% of respondents), income generation (20% of respondents) and cultural reasons (5% of respondents). 45% of the species recorded are still at early stage in domestication and only 2% are fully domesticated. Eleven factors related to the households surveyed and to the head of the household interviewed affect farmers’ decision making in domesticating plant species. There is gender influence on the domestication: Women are keen in domesticating herbs while men give priority to trees.

Sesamum radiatum and Justicia tenella are two traditional leafy vegetables highly consumed in Benin. In spite of their importance in food security, nutrition, and income generation, they still remain very little known to the scientific communities. In order to document their diversity and the various traditional cultural practices associated with their production and identity as well as their domestication levels across zones, a survey was conducted in 10 villages randomly selected from different agroecological and ethnic zones of both northern and central Benin. Data were collected in the different sites through application of Participatory Research Appraisal tools and techniques and analysed using both simple descriptive statistics (means, frequencies, etc.) and multivariate analysis (ANOVA, cluster analysis). The study revealed the existence of clear intraspecific diversity within J. tenella contrarily to S. radiatum for which no apparent diversity was noted. In most of the households surveyed, J. tenella and S. radiatum were found respectively at steps 3 and 4 in the domestication process. The production of these vegetable species is still traditional and biologic (no fertilisers, no pesticides). The cultural practices used are not the same for the two species and vary between households and between ethnic groups. The multivariate analyses (Cluster analysis, PCA) conducted based on the various traditional farming practices to examine the relationship between farmers revealed respectively 4 and 5 categories of producers of J. tenella and S. radiatum corresponding to the same numbers of applied traditional technological packages. Further domestication trials were recommended to develop the best technical packages required to master mass production of the species in the future for the benefit of both producers and consumers. Agromorphological and genetic characterisation were also recommended in order to establish the scientific basis for their varietal improvement. Key words: Sesamum radiatum, Justicia tenella, leafy vegetables, traditional production, domestication, Benin.

Many of the plant species that are cultivated for food across the world are neglected and underutilized. To assess their diversity in Benin and identify the priority species and establish their research needs, a survey was conducted in 50 villages distributed throughout the country. The study revealed 41 neglected and underutilized crop species (NUCS) among which 19 were identified as of priority base on 10 criteria among which included their extent and degree of consumption. Reasons for neglect vary with the producers and the agricultural technicians. Market surveys revealed that NUCS are important source of household incomes and substantially contribute to poverty reduction. Review of the literature available revealed that most of the species are rich in nutrients and have some proven medicinal values and the promotion of their use would help in combating malnutrition and improving the health status of the local populations. The knowledge gaps and research needs are immense on most of the species identified as no concrete scientific data is nationally available. In terms of research, almost all has to be done starting from basic ethnobotanical investigation. The results will help the scientists and students willing to conduct research on NUCS in Benin to better orient their research programs.

Acmella uliginosa, Ceratotheca sesamoides, Justicia tenella and Sesamum radiatum are four traditional leafy vegetables which are widely consumed in Benin. In order to document their origin, folk nomenclature, geographical distribution and ecology and to select adequate zones for in situ conservation of their genetic resources, 118 villages were randomly selected and surveyed throughout the country using a participatory research approach. All the species apart from Ceratotheca sesamoides were reported by farmers as introduced in Benin at different periods. The folk nomenclature comprising 93 different names is based on nine key criteria of variable frequencies according to the species. The geographical distribution maps revealed that the species under study are unequally distributed. Indeed, A. uliginosa is found exclusively in the northwest, J. tenella in the northeast and partially in the centre while S. radiatum and Ceratotheca sesamoides were found almost everywhere in Benin. Results from multivariate analysis combined with geographical distribution maps of the species and the soil and climate maps of Benin, leaded to the selection of the Northwest and of the Northeast regions as the appropriate conservation zones for the four species. Molecular analysis was recommended as it can help in describing genetic diversity and consequently in identifying additional sites for the widely spread species Sesamum radiatum and Ceratotheca sesamoides. Keywords: Folk nomenclature, biodiversity, geographical distribution, site selection, in situ conservation, Benin.