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Objectives: To examine the relationship between masticatory muscle activity (MMA), self-reported oral behaviours (OBs) and overall physical activity (PA) in adult women. Materials and methods: MMA and PA were assessed by a wearable electromyography (EMG) device and accelerometer respectively, worn over 2 non-consecutive days by 53 women (mean age 27.5 ± 6.4 years). Following the second recording day, self-reported OBs were assessed. MMA was assessed by the number, amplitude and duration of masseter contraction episodes. Masseter muscle EMG outcome measures were number of contraction episodes per hour (CEs/h) and the relative contraction time (RCT%). PA was assessed by time accumulated in moderate to vigorous physical activity (MVPA) and 10-min bouts of MVPA per hour. Data were analysed using mixed model analysis. Results: MMA in free-living conditions consisted mostly of low-amplitude (

Objective The aim of this case-control study was to investigate the association between non-syndromic hypodontia and nineteen common variants of candidate genes ectodysplasin A (EDA), paired box 9 (PAX9), msh homeobox 1 (MSX1), and axis inhibition protein 2 (AXIN2). Settings and sample population Sixty-one hypodontia cases were frequency-matched to 253 controls with no missing teeth (excluding the third molars). Material and methods Self-report data and DNA samples were collected from each participant. Results The sample had a mean age of 16.6 years (SD=7.3), with most participants being female (59.6%), and of New Zealand European origin (75.4%). Using multiple logistic regression analysis, it was found that the T-allele of rs12853659 (EDA) and the G-allele of rs2428151 (EDA) were both associated with a higher risk of hypodontia (odds ratio, OR = 2.79, 95% CI = 1.11-7.01; and OR = 2.87, 95% CI = 1.04-7.94, respectively). The G-allele of rs2520378 (EDA) showed a protective effect with an OR of 0.61 (95% CI = 0.38-0.99). The EDA SNP findings were consistent with previous reports included in a meta-analysis. No associations were found with the PAX9, AXIN2 and MSX1 genes, after adjusting for sex and ethnicity. Conclusions Common variants of the EDA genes are associated with specific phenotypes of non-syndromic hypodontia, thus confirming their role in the regulatory pathways of normal tooth development. However, larger samples are needed to investigate the association further.

Occlusal features that deviate from normative values have been historically considered risk factors for temporomandibular joint (TMJ) disorders. Nowadays, a putative association between dental occlusion and TMJ disorders remains controversial, with research findings on associations being inconsistent and inconclusive. We hypothesized that putative occlusal features identified during adolescence are associated with TMJ clicking later in life. The Dunedin Multidisciplinary Health and Development Study is a longitudinal birth cohort study investigation of 1,037 children (48.4% female) born in Dunedin, New Zealand, between April 1, 1972, and March 31, 1973, and assessed repeatedly since then. Associations between posterior crossbite, overbite, and overjet at age 15, as well as both self-reported and clinically assessed TMJ clicking sounds at age 45, were studied. Data were analyzed using multivariate logistic regression, after controlling for sex, emotional style, self-reports of tooth clenching and sleep bruxism, and history of orthodontic treatment. Self-reported and examiner-reported TMJ clicking at age 45 affected 18.3% and 23.8% of the study sample, respectively, and were not associated with the presence of a posterior crossbite or abnormal overjet/overbite values during adolescence. Self-reported history of tooth clenching and emotional style were associated with self-reported TMJ clicking later in life. In addition, there is a suggestion that high overbite during adolescence is negatively associated with TMJ clicking later in life. A history of orthodontic treatment was not associated with TMJ clicking. Abnormal occlusal features, such as posterior crossbite and high and low overjet/overbite in adolescence, are not associated with higher prevalence of TMJ clicking later in life. Personality also appears to influence self-reports of TMJ clicking later in life.

Indigenous youth are systemically underserved by 4-H and other positive youth development (PYD) organizations. Many underserved First Nation communities in the United States could greatly benefit from programs that foster youth thriving; however, these programs tend to be ineffective in creating culturally reflective spaces for Indigenous participants. In this article, we argue that the Peoplehood Model should serve as a unifying model for the inclusion of Indigenous identity in programming, and that cultural humility should be firmly integrated into program design and assessment. We also propose that, to support Indigenous youth thriving, PYD practitioners must intentionally create a “partial vacuum” that supports youth creating program context and thriving.

SummaryObjectivesTo assess biomechanical properties of the lip muscles and to investigate their relationship with sex, age, body mass index (BMI), and cephalometric variables.Materials and methodsDemographic information and BMI were collected from 83 study participants seeking orthodontic treatment at the University of Otago. Tone, stiffness, and elasticity of the lip muscles were measured thrice at four different anatomical sites using a digital palpation device. To estimate method error, a duplicate set of measurements in a subsample of 20 participants was taken a week later. Sagittal and vertical cephalometric classifications were based on ANB and FMPA angles, respectively. Data were analysed using mixed models.ResultsBiomechanical properties of lip muscles were remarkably consistent both within and between different recording sessions. The lower lip had higher tone and stiffness than the upper lip. Both the upper and lower lip stiffness and tone were higher in females than in males, whereas upper lip elasticity was higher in males. Thinner upper lips had higher tone and were stiffer than thicker upper lips, whereas thinner lower lips were less elastic than thicker lower lips. Muscle tone and stiffness of both the upper and lower lips were lower in Class III than in Class I and Class II individuals. The upper lip of hyperdivergent individuals was less elastic than that of normodivergent and hypodivergent individuals, and stiffer than that of hypodivergent individuals.ConclusionsThe biomechanical properties of perioral soft tissues can be reliably measured and vary with anatomical site, sex, and cephalometric measurements.

To describe epidemiological and clinical features of patients confirmed as having SARS-CoV-2 infection and managed in isolation at home. We performed a multicenter retrospective study enrolling all SARS-CoV-2-positive adults evaluated from 28 February to 31 May 2020 at one of nine COVID-19 Units in southern Italy: we included patients receiving care at home and those admitted to hospital. We defined patients with not-severe disease if they were asymptomatic or experienced a mild infection that did not need oxygen (O2) therapy and those with a severe infection if hospitalized and required O2 therapy. We enrolled 415 patients with SARS-CoV-2 infection: 77 were managed in isolation at home, 338 required hospital management. The 77 patients in home isolation were less frequently male than hospitalized patients (55% vs. 64%; < 0.01) and were younger (median age 45 years (IQR:19) vs 62 (IQR 22); p < 0.01), had a lower Charlson comorbidity index (median 0 (IQR2) vs 6 (IQR 3); p < 0.01), and included fewer subjects with an underlying chronic disease (36% vs 59%; p < 0.01). According to a binomial logistic regression analysis, a younger age (OR: 0.96 (95% IC: 0.94–0.98), p < 0.01) and a low Charlson comorbidity index (OR: 0.66 (95% IC: 0.54 –0.83); p < 0.01) were independent factors associated with at-home management. The identification of subjects with SARS-CoV-2 infection who could be managed in home isolation is useful in clinical practice. A younger age and no comorbidities were identified as factors independently associated with home management.

In the last years, the application of artificial intelligence (Machine Learning and Deep Learning methods) for the classification of 3D point clouds has become an important task in modern 3D documentation and modelling applications. The identification of proper geometric and radiometric features becomes fundamental to classify 2D/3D data correctly. While many studies have been conducted in the geospatial field, the cultural heritage sector is still partly unexplored. In this paper we analyse the efficacy of the geometric covariance features as a support for the classification of Cultural Heritage point clouds. To analyse the impact of the different features calculated on spherical neighbourhoods at various radius sizes, we present results obtained on four different heritage case studies using different features configurations.

Objectives: To analyze changes in occlusal characteristics following mandibular incisor extractions (MIE), to determine the usefulness of wax setups in treatment planning MIE cases and to compare the pre- and posttreatment dental attractiveness between MIE cases and nonextraction (NE) controls. Materials and Methods: The Peer Assessment Rating (PAR) Index was used to score pre- and posttreatment dental casts of MIE cases (n = 14) and matched NE controls (n = 14). Occlusal characteristics were evaluated on diagnostic wax setups and posttreatment casts. Attractiveness of pre- and posttreatment cases judged on intraoral photographs of cases (n = 6) and controls (n = 6) were rated by 76 dental students and 10 laypeople using visual analogue scales (VAS). Results: The difference in PAR score reduction (%) between the MIE and NE groups was not significant. Between the wax setup and posttreatment casts, there were moderate correlations in overjet, overbite, and right canine classification. There was no significant difference in pre- and posttreatment change in VAS scores (%) for attractiveness between the MIE (49.8 ± 4.3 [S.E.]) and control groups (40.8 ± 4.3 [S.E.]). However, there was a significant difference (P = .000) between the observer groups. Conclusions: There were no significant differences in the treatment outcomes of orthodontic cases treated with MIE or NE, indicating that MIE is a valid treatment option. A wax setup is moderately correlated with posttreatment results. Both laypeople and dental students rated posttreatment dental attractiveness higher than pretreatment in MIE and NE groups. Dental students tended to be more critical than laypeople in their ratings.

Objective To assess the relationship between history of surgery for endometriosis and adverse obstetrical outcomes. Design Retrospective study using prospectively recorded data. Setting Referral center. Patient(s) Total of 569 women with history of surgery for endometriosis, postoperative conception, and pregnancy evolution over 22 weeks of gestation. Interventions(s) Surgery for endometriosis. Main Outcome Measure(s) Small for gestational age (SGA) status of the newborn, spontaneous preterm birth (PT, before 37 weeks’ gestation), and placenta previa. Results Among 733 pregnancies included in the study, 566 deliveries were recorded (77.2%), of which 535 were singleton (72.9% of pregnancies) and 31 twins (4.2%). SGA was observed in 81 of 535 (15.1%) singleton pregnancies and in 9 of 31 (29%) twin pregnancies. PT occurred in 53 of 535 (9.9%) singleton pregnancies and in 19 of 31 (61.2%) twin pregnancies. The number of singleton and multiple pregnancies complicated by placenta previa were, respectively, 9 of 535 (1.7%) and 0 of 31. The independent factor found to relate to SGA was the absence of endometriomas; conception with the use of assisted reproductive technologies (ART) only tended toward statistical significance. Independent factors found to increase risk of PT were conception with the use of ART, body mass index >30 kg/m2, and surgery of deep endometriosis infiltrating the rectum and the bladder. Independent factors associated with placenta previa were conception with the use of ART and history of stage III or IV endometriosis. Conclusion(s) The risk of SGA and PT is increased in women with a history of surgery for endometriosis, and a high rate of conception with the use of ART may jeopardize outcomes.

Background Emerging sensing and communication technologies are contributing to the development of many motor rehabilitation programs outside the standard healthcare facilities. Nowadays, motor rehabilitation exercises can be easily performed and monitored even at home by a variety of motion-tracking systems. These are cheap, reliable, easy-to-use, and allow also remote configuration and control of the rehabilitation programs. The two most promising technologies for home-based motor rehabilitation programs are inertial wearable sensors and video-based motion capture systems. Methods In this paper, after a thorough review of the relevant literature, an original experimental analysis is reported for two corresponding commercially available solutions, a wearable inertial measurement unit and the Kinect, respectively. For the former, a number of different algorithms for rigid body pose estimation from sensor data were also tested. Both systems were compared with the measurements obtained with state-of-the-art marker-based stereophotogrammetric motion analysis, taken as a gold-standard, and also evaluated outside the lab in a home environment. Results The results in the laboratory setting showed similarly good performance for the elementary large motion exercises, with both systems having errors in the 3–8 degree range. Usability and other possible limitations were also assessed during utilization at home, which revealed additional advantages and drawbacks for the two systems. Conclusions The two evaluated systems use different technology and algorithms, but have similar performance in terms of human motion tracking. Therefore, both can be adopted for monitoring home-based rehabilitation programs, taking adequate precautions however for operation, user instructions and interpretation of the results.

Study Objective: To assess the risk of low anterior resection syndrome (LARS) between women managed by either disk excision or rectal resection for low rectal endometriosis. Design: Retrospective study of a prospective database. Setting: University hospital. Patients: One hundred seventy-two patients managed by disk excision or rectal resection for deep endometriosis infiltrating the rectum

Patients with masticatory muscle pain and migraine typically report that the intensity of pain fluctuates over time and is affected by weather changes. Weather variables, such as ambient temperature and humidity, may vary significantly depending on whether the individual is outdoor or indoor. It is, therefore, important to assess these variables at the individual level using portable monitors, during everyday life. This study aimed to determine and compare the temporal patterns of pain in individuals affected with facial and head pain and to investigate its relation with weather changes. Eleven patients (27·3 ± 7·4 years) with chronic masticatory muscle pain (MP) and twenty (33·1 ± 8·7 years) with migraine headache (MH) were asked to report their current pain level on a visual analogue scale (VAS) every hour over fourteen consecutive days. The VAS scores were collected using portable data-loggers, which were also used to record temperature, atmospheric pressure and relative humidity. VAS scores varied markedly over time in both groups. Pain VAS scores fluctuate less in the MP group than in the MH group, but their mean, minimum and maximum values were higher than those of migraine patients (all PÂ

Background Behavioural aspects of chewing may influence food intake, nutritional status and in turn body weight. Objectives The current study aimed to study chewing features in adolescents as they naturally occur in home-based settings, and to test for a possible association with weight status. Methods Forty-two adolescents (15.3 ± 1.3 years) were recruited (21 with healthy-weight/21 with overweight). Using a smartphone-assisted wearable electromyographic device, the chewing features of each participant were assessed over one evening, including the evening meal, in their natural home setting. Results The mean (±SD) for chewing pace was 1.53 ± 0.22 Hz, chewing power 30.1% ± 4.8%, number of chewing episodes 63.1 ± 36.7 and chewing time 11.0 ± 7.7 minutes. The chewing pace of the group with overweight was slower than that of healthy weight (-0.20 Hz; 95% CI, -0.06 to -0.33; P = .005) while their chewing time was shorter (-4.9 minutes; 95% CI, 0.2-9.7; P = .044). A significant negative correlation was observed between BMI z-score and chewing pace (R = -.41; P = .007), and between BMI z-score and chewing time (R = -0.32; P = .039). Conclusion The current study suggests that adolescents who are overweight eat at a slower pace for a shorter period of time than their counterparts who are a healthy weight. This unexpected finding based on objective data appears to conflict with existing questionnaire findings but provides impetus for further work testing the effectiveness of changing eating behaviour as a weight-management intervention in youth.

In this prospective study, we investigated the structural and vascular retinal changes at baseline and after Ranibizumab injections at the last follow up to one year in patients affected by Radiation Maculopathy (RM) after plaque brachytheraphy in choroidal melanoma, using Spectral Domain Optical Coherence Tomography (SD-OCT) and OCT Angiography (OCTA). A total of 40 eyes with RM of 40 patients (18 females, 22 males, mean age 51.9 &plusmn, 11 years) that underwent ruthenium-106 plaque brachytherapy were included. All patients received one monthly intravitreal injection of Ranibizumab (Pro Re Nata regimen). We analyzed the Foveal Avascular Zone (FAZ) area, the retinal vessel density (VD) of the superficial capillary plexus (SCP) and of the deep capillary plexus (DCP), using OCTA, and we detected the Central Foveal Thickness (CFT) by SD-OCT at baseline and after treatment. At the last follow up, we found a significant improvement of the CFT (p &lt, 0.001) while OCTA parameters revealed no change in VD of the SCP (p = 0.402), DCP (p = 0.282), and FAZ area (p = 0.255), resulting in a stabilization of the visual acuity (p = 0.210) respect to baseline. The absence of functional improvement, despite the anatomical recovery of the macula, could be due to the absence of improvement in FAZ area and in retinal VD after treatment. OCTA parameters could represent predictive biomarkers to anti-vascular endothelial growth factor (anti-VEGF) intravitreal response and to help to better understand the physiopathological mechanisms of the RM.

The purpose of this study was to determine the feasibility of a smartphone application (app) to assess pain levels in real life, and to test their association with gender, age, time in orthodontic treatment, and type of orthodontic adjustment. Eighty-two participants undergoing orthodontic treatment were recruited. A newly developed app was used to assess pain scores at regular intervals in the three days after adjustment of braces. Resting and chewing pain were assessed using sliding digital visual analogue scales. The mean age of the sample was 15.2 ± 1.6years, the mean time in treatment was 12 ± 8.4 months, and the majority (56.1%) were females. Resting pain and chewing pain at the teeth rose steadily from baseline, peaked at approximately 20hours, then decreased gradually over the next two days. Details of the orthodontic adjustments were associated with the total pain experienced at the teeth, with new bond-ups resulting in significantly more pain than routine orthodontic adjustments. Pain levels were not significantly associated with age, gender, or time in treatment. This smartphone app shows promise in measuring orthodontic pain in the real world, and will aid future research projects which investigate various factors that could influence pain severity.

Introduction: Although sorafenib is the upfront standard of care for advanced hepatocellular carcinoma (HCC), molecular predictors of efficacy have not been identified yet. In the ALICE-1 study, rs2010963 of VEGF-A and VEGF-C proved to be independent predictive factors for progression-free survival (PFS) and overall survival (OS) in multivariate analysis. The ALICE-1 study results were confirmed in the ALICE-2 study, in which VEGF and VEGFR SNPs were analyzed. In the ePHAS study we analyzed the SNPs of eNOS. In univariate analysis, patients homozygous for an eNOS haplotype (HT1: T-4b at eNOS-786/eNOS VNTR) had significantly shorter median PFS and OS than those with other haplotypes. These data were confirmed in the validation set. Methods: This nonpharmacological, interventional, prospective multicenter study aims to determine whether eNOS, HIF-1, VEGF, Ang2 and VEGFR polymorphisms play a role in predicting the objective response rate, PFS, and OS of advanced HCC patients treated with sorafenib. The study will involve 160 advanced HCC patients with Child-Pugh class A disease. The primary aim is to validate the prognostic or predictive roles of eNOS, Ang2, HIF-1, VEGF and VEGFR polymorphisms in relation to the clinical outcome (PFS) of HCC patients treated with sorafenib. Conclusions: Overall, our data may suggest that polymorphism analysis of the VEGF, VEGFR-2, HIF and eNOS genes can identify HCC patients who are more likely to benefit from sorafenib.

Child independent mobility (CIM) refers to the freedom and capability of children to move about their local neighborhoods without constant direct adult supervision. Our climb project combats an observed decline in CIM, offering a pervasive gameful platform for home–school mobility composed of three primary components: the first two using technology to support different levels of child independence and the third providing an element of continuous motivation for positive behavior change. This paper describes these three novel technologies: PedibusSmart, SafePath, and KidsGoGreen, and reports on four years of success with more than 1800 elementary age children, their teachers, and families. We further show how (i), disappearing, pervasive technology contributes to successful adoption, (ii), properly balancing trust and tracking leads to useful, noninvasive technological support, and (iii), in-classroom, gameful technology engages and motivates participation, with behavior changes persisting over time.

This work presents an extended photogrammetric pipeline aimed to improve 3D reconstruction results. Standard photogrammetric pipelines can produce noisy 3D data, especially when images are acquired with various sensors featuring different properties. In this paper, we propose an automatic filtering procedure based on some geometric features computed on the sparse point cloud created within the bundle adjustment phase. Bad 3D tie points and outliers are detected and removed, relying on micro and macro-clusters analyses. Clusters are built according to the prevalent dimensionality class (1D, 2D, 3D) assigned to low-entropy points, and corresponding to the main linear, planar o scatter local behaviour of the point cloud. While the macro-clusters analysis removes smallsized clusters and high-entropy points, in the micro-clusters investigation covariance features are used to verify the inner coherence of each point to the assigned class. Results on heritage scenarios are presented and discussed.

Augmented Reality (AR) is already transforming many fields, from medical applications to industry, entertainment and heritage. In its most common form, AR expands reality with virtual 3D elements, providing users with an enhanced and enriched experience of the surroundings. Until now, most of the research work focused on techniques based on markers or on GNSS/INS positioning. These approaches require either the preparation of the scene or a strong satellite signal to work properly. In this paper, we investigate the use of visual-based methods, i.e., methods that exploit distinctive features of the scene estimated with Visual Simultaneous Localization and Mapping (V-SLAM) algorithms, to determine and track the user position and attitude. The detected features, which encode the visual appearance of the scene, can be saved and later used to track the user in successive AR sessions. Existing AR frameworks like Google ARCore, Apple ARKit and Unity AR Foundation recently introduced visual-based localization in their frameworks, but they target mainly small scenarios. We propose a new Mobile Augmented Reality (MAR) methodology that exploits OPEN-V-SLAM to extend the application range of Unity AR Foundation and better handle large-scale environments. The proposed methodology is successfully tested in both controlled and real-case large heritage scenarios. Results are available also in this video: https://youtu.be/Q7VybmiWIuI.

BACKGROUND Little is known about wake-time masseter activity of patients with masticatory muscle pain in real-life settings. OBJECTIVES The aims of this study were (1) to assess wake-time masseter activity of women with masticatory muscle pain (MMP) and compare it with that of pain-free women; and (2) to investigate the association between objectively measured masseter contractions and self-reported oral behaviours. METHODS Adult women (N = 27) diagnosed with MMP were age-matched with pain-free women (N = 26). The electromyographic (EMG) activity of the masseter muscle when awake was recorded unilaterally for two consecutive days. The number of contraction episodes and relative contraction time (%) were calculated using cut-off thresholds of 3%, 5% and 10% of maximum voluntary contraction (MVC). Self-reported daytime oral activity was assessed using the Oral Behaviours Checklist (OBC, items 3-21). Data were analysed using univariate tests and mixed-model analyses. RESULTS The number of contraction episodes was similar between groups for all detection thresholds, but the relative contraction time at the lower detection thresholds (i.e. 3% MVC and 5% MVC) was significantly longer (1.5-fold) in the MMP than in the pain-free group. The OBC scores were significantly higher in the women with MMP than in controls, but no positive association could be found between EMG variables and OBC scores. CONCLUSION Women with MMP engage in low-level masseter muscle contractions, which last longer than those of pain-free controls. There was no relationship between self-reported oral behaviours and masseter activity when awake. These findings support the notion that prolonged low-level clenching when awake may play a role in the pathophysiology of MMP.

Background Many modifications to fixed orthodontic appliances have been introduced to manage biofilm formation. The aim of this review was to investigate elastomeric ligation in comparison to stainless steel ligation, and self-ligation with regards to microbiological and clinical indicators of biofilm formation in patients wearing multi-bracketed fixed orthodontic appliances. Methods The MEDLINE and the EMBASE databases were searched up to February 2021 and supplemented by additional manual searches of bibliographies. Parallel-group and split-mouth randomised controlled trials (RCTs) comparing different ligation methods were identified. The Cochrane Risk of Bias-2 tool was applied to assess the quality of evidence. Results A total of 11 RCTs were included in this review. Nine RCTs compared self-ligation and elastomeric ligation; two compared elastomeric ligation and stainless-steel ligation. The included studies had either some concerns or were at a high risk of bias. Qualitative assessment of the studies identified that there were no significant differences in biofilm formation between elastomeric ligation and self-ligation, but that stainless-steel ligation was less susceptible to biofilm formation than elastomeric ligation. Conclusions There were no significant differences between self-ligation and elastomeric ligation for biofilm formation in patients wearing multi-bracketed fixed orthodontic appliances. Stainless-steel ligation may accumulate less biofilm than elastomeric ligation, however the clinical significance of the difference could not be evaluated. Further high-quality studies are required in order to determine which ligation method is better for managing biofilm formation in patients wearing multi-bracketed fixed orthodontic appliances.

Background Pain experienced at teeth during orthodontic treatment varies largely over time, with the reasons for its interindividual variability being largely unknown: age, sex, clinical activations, psychosocial factors and genetic polymorphisms of candidate genes are putative factors that may account to explain this variability. We aimed to investigate the effect of clinical, demographic, psychological and genetic factors on pain levels experienced during fixed orthodontic treatment. Methods A convenience sample of 183 patients undergoing full-fixed orthodontic treatment were recruited. Participant's pain levels were assessed seven times over a three-day period via a smartphone app. Clinical, demographic and psychological data were collected via questionnaires. This included the Pain Catastrophising Scale (Child version), the Corah Dental Anxiety Scale and the State and Trait Anxiety Inventory. Participants provided a DNA sample either in the form of blood or saliva, which were used for genotyping COMT gene rs6269, rs4680, rs4646310, NR3C1 gene rs2963155 and the HTR2A gene rs9316233. Results Bond ups had the greatest influence on perceived levels of pain experienced on teeth during orthodontic treatment, accounting for over 20% of total variance in pain response. High-pain responders had higher scores on pain catastrophising (magnification subscale). Self-reported pain during fixed orthodontic treatment was not influenced by sex, age, time into treatment, anxiety, nor by polymorphisms of COMT, HTR2A or NR3C1 genes. Conclusions Pain on teeth resulting from orthodontic fixed appliances is stronger during bonds-up and in patients with high catastrophising scores. Demographics, type of clinical activations and the genetic polymorphisms investigated in this research had little or no impact on perceived pain levels.

We have performed a study on the thermo-formation process of plastic shells of boots for winter sports. The aim of the work has been to determine how the process parameters can improve the ergonomic and thermal comfort for athletes and winter sports participants. We have found that the initial deformation and memory effect are strongly influenced by the type of thermoplastic material used, heating time and cooling procedure. We have also found that the ovens currently used for this process are not correctly designed to obtain an efficient heat transfer and, therefore, a heating time of at least 12 min is necessary to achieve an average deformation of more than 2 mm. The measure of the boot flexural stiffness has shown that there are no changes in the load–deflection behavior after the thermo-formation process of a ski boot made of thermoplastic polyurethane. We have also found that the thermo-formation process decreases the pressure on skier’s feet of more than five times and, therefore, improves the comfort of the skier.

Reduced periodontal support is a challenge that clinicians often face during rehabilitation of compromised dentition. The close and intricate relationship between the periodontal tissues and the processes of tooth movement suggest that adjunct orthodontic therapy may play an important role in overcoming these problems. On the other hand, excessive movement of teeth beyond the anatomic boundaries of the alveolar process is commonly believed to contribute to further destruction of the periodontal tissues. This review evaluates the clinical effects of various orthodontic tooth movements on the surrounding periodontal soft tissues and alveolar bone. Another objective was to identify possible patient and treatment-related factors that may influence the response of periodontal tissue to specific orthodontic treatments. Particular emphasis is placed on specific tooth movements, such as extrusion, intrusion, space closure and arch expansion. Limitations of current research are also highlighted and discussed.

Introduction Interproximal reduction (IPR) removes enamel and leaves grooves and furrows on the tooth surface, which may increase the risk of caries. The aims of this study were to assess the nanotopography of enamel surfaces produced by the most commonly used IPR instruments and to evaluate the effect of polishing after IPR. Methods Enamel slabs were cut from the interproximal surfaces of healthy premolars and then treated with diamond burs, strips, or discs, or Sof-Lex polishing discs (3M ESPE, St Paul, Minn). All samples were cleaned by sonication in distilled water. The control group had no IPR performed and was subjected only to cleaning by sonication. The enamel surfaces were assessed using atomic force microscopy. Results The IPR instruments all produced surfaces rougher than the control sample; however, the samples that received polishing with Sof-Lex discs after enamel reduction were smoother than untreated enamel (P

During growth the mandible accommodates increases in biomechanical loading resulting from changes in the function of structures of the oral cavity. Biomechanical loads are thought to play an intricate and vital role in the modelling and remodelling of bone, with site-specific effects on bone mineral density. It is anticipated that the effects of this loading on bone mineral density are intensified during the functional transition from prenatal to postnatal stages. The aim of this study was thus to evaluate changes in bone mineral density across the body of the immature human mandible during the early stages of dental development. The study sample included 45 human mandibles, subdivided into three age groups: prenatal (30 gestational weeks to birth; n = 15); early postnatal (birth to 12 months; n = 18); and late postnatal (1-5 years; n = 12). Mandibles were scanned using X-ray micro-computed tomography. Eight landmarks were selected along the buccal/labial and lingual surfaces of each dental crypt for evaluation of the bone mineral density. Bone mineral density values were calculated using a reference standard and analysed using multivariate statistics. The bone mineral density of the lingual surface was found to be significantly higher (P ≤ 0.000) than that of the buccal/labial surface. Furthermore, bone mineral density in the alveolar region of the buccal/labial surface of the deciduous central incisor (P ≤ 0.001), the deciduous first molar (P ≤ 0.013) and lingual alveolar area of the deciduous second molar (P ≤ 0.032) were significantly greater in the early postnatal period than in the prenatal period. While changes in bone mineral density across the lingual surface were consistent with the progression of development and the biomechanical demand of the tongue as previously demonstrated, changes observed across the buccal/labial surface of the mandible appeared to accompany the advancing dental development. Thus, changes in bone mineral density across the mandible appear to be reflective of the stage of dental development and the level of biomechanical loading.

Background The functional outcome of total knee replacement (TKR) is usually satisfying. However, patients may show functional limitations for years after surgery, which have been ascribed to impairments in balance and proprioception, mainly during standing tasks. A number of instrumentations and parameters have been used, rising confusion for clinical decisions on the assessment of patients. Research question Which are the most widespread and consistent procedures to assess balance and proprioception following TKR? Methods A literature review was conducted in Pubmed, PEDro, and Cochrane database. From a total sample of 112 articles, 23 original studies published between 2008 and 2019 met inclusion criteria. The primary outcomes selected were variables related to balance and proprioception assessment in static and dynamic tasks performed with instrumented platforms. Data from papers using the same instrumentation, on patients with unilateral TKA and at least 12 months postoperatively were synthesized quantitatively in a random effect meta-analysis. Results Fourteen articles were appropriate for the review. A large variability was found both in the instrumentation and the parameters used. The Neurocom Balance Master System™ was the most used instrument (four articles). On a total population of 186 patients with unilateral TKR 12 months postoperatively, a low degree of heterogeneity was found adopting the random effect in the four tasks explored (Firm and Foam Surface both with Eyes Open and Eyes Closed). Significance This review found a large variability in the instrumentation used to assess balance and proprioception in patients operated on TKR. The meta-analysis demonstrated that the Neurocom Balance Master System™ for static assessment of balance showed an acceptable consistency and can be considered as a reference for further studies. However, balance and proprioception impairments following TKR have not been widely quantified by means of instrumented platforms. Further research is needed to address this issue, and improve clinical practice.

Objective To assess the immediate response of lips in three dimensions (3D) resulting from simulated maxillary incisor advancement. Materials and Methods Incremental maxillary incisor advancement was simulated by placing wax of increasing thickness (+2 mm, +4 mm, +6 mm) on the incisors of 20 participants, and the induced lip changes were recorded using 3D stereophotogrammetry. The induced displacement of lip landmarks was quantified using 3D image analysis software. Data were analyzed using a repeated-measures analysis of variance (ANOVA) after adjusting for age and sex of the study participants. Results A large interindividual variation in lip response to simulated incisor advancement was observed. A significant overall effect on 3D lip changes was found for increasing values of simulated incisor advancement (F = 13.2; P < .001) as well as significant differences between anatomical landmarks of the lip (F = 7.4; P < .01). Most points moved outward and anterosuperiorly, except the midpoint and corners of the lip. Greatest movement was observed in the sagittal plane, followed by the vertical and transverse planes. Conclusions Maxillary incisor advancement significantly affects upper lip change in three planes of space: particularly the anteroposterior plane, in which the response to simulated advancement appears to be nonlinear.

Halitosis, an offensive breath odour, has multiple sources and negative impacts on people's social interactions and quality of life. It is important for health care professionals, including general physicians and dental professionals, to understand its aetiology and risk factors in order to diagnose and treat patients appropriately. In this study, we have reviewed the current literature on halitosis regarding its prevalence, classification, risk factors, sources, measurement and treatment.

Outdoor acoustic event detection is an exciting research field but challenged by the need for complex algorithms and deep learning techniques, typically requiring many computational, memory, and energy resources. These challenges discourage IoT implementations, where an efficient use of resources is required. However, current embedded technologies and microcontrollers have increased their capabilities without penalizing energy efficiency. This paper addresses the application of sound event detection at the very edge, by optimizing deep learning techniques on resource-constrained embedded platforms for the IoT. The contribution is two-fold: firstly, a two-stage student-teacher approach is presented to make state-of-the-art neural networks for sound event detection fit on current microcontrollers; secondly, we test our approach on an ARM Cortex M4, particularly focusing on issues related to 8-bits quantization. Our embedded implementation can achieve 68% accuracy in recognition on Urbansound8k, not far from state-of-the-art performance, with an inference time of 125 ms for each second of the audio stream, and power consumption of 5.5 mW in just 34.3 kB of RAM.

In most classification tasks, wide and deep neural networks perform and generalize better than their smaller counterparts, in particular when they are exposed to large and heterogeneous training sets. However, in the emerging field of Internet of Things memory footprint and energy budget pose severe limits on the size and complexity of the neural models that can be implemented on embedded devices. The Student-Teacher approach is an attractive strategy to distill knowledge from a large network into smaller ones, that can fit on low-energy low-complexity embedded IoT platforms. In this paper, we consider the outdoor sound event detection task as a use case. Building upon the VGGish network, we investigate different distillation strategies to substantially reduce the classifier's size and computational cost with minimal performance losses. Experiments on the UrbanSound8K dataset show that extreme compression factors (up to 4.2 · 10−4 for parameters and 1.2 · 10−3 for operations with respect to VGGish) can be achieved, limiting the accuracy degradation from 75% to 70%. Finally, we compare different embedded platforms to analyze the trade-off between available resources and achievable accuracy.

Detection of human presence is a key feature in Human Computer Interaction. Solutions based on cameras are attractive, but require computer vision techniques to extract meaningful data, which can be expensive from a computational point of view. In this work, we present a new system that merges a low resolution thermal camera with advanced feature extraction techniques such as Convolutional Neural Networks. We demonstrate the possibility to adapt their execution to resource-constrained platform without significant loss of performance, by processing data on a 32-bit low power microcontroller, performing the classification on thermal video stream. It achieve 76.7% of accuracy in the microcontroller, requiring only 16.5 mW in continuous classification mode and using 6 kB of RAM.

Orthodontic braces can impede oral hygiene and promote halitosis. The aim of the study was to investigate the effect of the oral probiotic Streptococcus salivarius M18 on oral hygiene indices and halitosis in patients wearing orthodontic braces. The study was a prospective, randomized, triple-blind, placebo-controlled trial. Patients undergoing fixed orthodontic treatment were randomly allocated to a probiotic group (n = 32) and a placebo group (n = 32). Patients consumed 2 lozenges d-1 for one month. Assessments were taken at baseline, at the end of the intervention, and at a 3 month follow-up. The outcome measures were plaque index (PI), gingival index (GI) and halitosis-causing volatile sulfur compound (VSC) levels. The dental biofilms before and after the intervention were analyzed utilizing next-generation sequencing of bacterial 16S rRNA genes. PI and GI scores were not significantly influenced by the probiotic intervention (intervention × time: p > 0.05). The level of VSCs decreased significantly in both the probiotic group (VSC reduction = -8.5%, 95%CI = -7.4% to -9.1%, p = 0.015) and the placebo group (-6.5%, 95%CI = -6.0% to -7.4%, p = 0.039) after 1 month intervention. However, at the 3 month follow-up, the VSC levels in the placebo group returned to baseline levels whereas those in the probiotic group decreased further (-10.8%, 95%CI = -10.5% to -12.9%, p = 0.005). Time, but not treatment, was associated with the decrease in microbial community alpha diversity and a modest effect on beta diversity. Oral probiotic S. salivarius M18 reduced the level of halitosis in patients with orthodontic braces, but had minimal effects on PI, GI and dental biofilm microflora.

The increasing importance of three-dimensional (3D) city modelling is linked to these data’s different applications and advantages in many domains. Images and Light Detection and Ranging (LiDAR) data availability are now an evident and unavoidable prerequisite, not always verified for past scenarios. Indeed, historical maps are often the only source of information when dealing with historical scenarios or multi-temporal (4D) digital representations. The paper presents a methodology to derive 4D building models in the level of detail 1 (LoD1), inferring missing height information through machine learning techniques. The aim is to realise 4D LoD1 buildings for geospatial analyses and visualisation, valorising historical data, and urban studies. Several machine learning regression techniques are analysed and employed for deriving missing height data from digitised multi-temporal maps. The implemented method relies on geometric, neighbours, and categorical attributes for height prediction. Derived elevation data are then used for 4D building reconstructions, offering multi-temporal versions of the considered urban scenarios. Various evaluation metrics are also presented for tackling the common issue of lack of ground-truth information within historical data.

Significance As residential development continues into flammable landscapes, wildfires increasingly threaten homes, lives, and livelihoods in the so-called “wildland–urban interface,” or WUI. Although this problem seems distinctly modern, Native American communities have lived in WUI contexts for centuries. When carefully considered, the past offers valuable lessons for coexisting with wildfire, climate change, and related challenges. Here we show that ancestors of Native Americans from Jemez Pueblo used ecologically savvy intensive burning and wood collection to make their ancient WUI resistant to climate variability and extreme fire behavior. Learning from the past offers modern WUI communities more options for addressing contemporary fire challenges. Public/private–tribal partnerships for wood and fire management can offer paths forward to restore fire-resilient WUI communities., The intersection of expanding human development and wildland landscapes—the “wildland–urban interface” or WUI—is one of the most vexing contexts for fire management because it involves complex interacting systems of people and nature. Here, we document the dynamism and stability of an ancient WUI that was apparently sustainable for more than 500 y. We combine ethnography, archaeology, paleoecology, and ecological modeling to infer intensive wood and fire use by Native American ancestors of Jemez Pueblo and the consequences on fire size, fire–climate relationships, and fire intensity. Initial settlement of northern New Mexico by Jemez farmers increased fire activity within an already dynamic landscape that experienced frequent fires. Wood harvesting for domestic fuel and architectural uses and abundant, small, patchy fires created a landscape that burned often but only rarely burned extensively. Depopulation of the forested landscape due to Spanish colonial impacts resulted in a rebound of fuels accompanied by the return of widely spreading, frequent surface fires. The sequence of more than 500 y of perennial small fires and wood collecting followed by frequent “free-range” wildland surface fires made the landscape resistant to extreme fire behavior, even when climate was conducive and surface fires were large. The ancient Jemez WUI offers an alternative model for fire management in modern WUI in the western United States, and possibly other settings where local management of woody fuels through use (domestic wood collecting) coupled with small prescribed fires may make these communities both self-reliant and more resilient to wildfire hazards.

Human pressures on marine ecosystems significantly increased during last decades. Among the intense anthropic activities, industrial fisheries have caused the alteration of habitats, the reduction of biodiversity and the main fish stocks. The aim of this research, carried out in the Adriatic Sea, was to test a repeatable Marine Spatial Planning framework aimed at enhancing fisheries sustainability through the application of Decision Support Tools and the composition of a catalog of possible measures. The use of these tools proved very useful to identify possible criticalities and facilitate an effective exchange with fisheries stakeholders, local authorities, and fishermen, whose involvement was an indispensable step in the process. Tool-based analyses allowed to assess the spatial footprint of a range of anthropogenic pressures from human activities (e.g., fisheries, maritime traffic, and aquaculture). Within this multi-pressure scenario, special attention was paid to fishing-related disturbances and potential conflicts across different fishing métier and with other sectors. Specifically, results highlighted the spatial features of the major fishing pressures (e.g., abrasion from trawling) affecting essential fish habitats, marine mammals and turtles in the study area. A portfolio of possible management measures is identified for the study area. It provides clear evidence that, in order to mitigate emerging conflicts and cumulative impacts, it is necessary to combine and integrate different types of measures: spatial measures modulated over time, monitoring and control, actions to fill knowledge gaps, concertation—involvement—co-management actions, improvement of governance systems, actions to support innovation in the sector, etc. Given the complex set of measures discussed, this work can provide a useful contribution to the management of fisheries both at local and regional level, fostering the transition to sustainable fisheries.

Retention may be particularly challenging after the correction of specific malocclusion features, such as tooth rotations, open bite and expansion, which are all inherently unstable. In this article, some indications for active retention are reviewed by highlighting a variety of clinical techniques and appliances. Active retention is discussed in relation to preservation of tooth alignment and in the three planes of space: sagittal, vertical and transverse. In some situations, an active retention regimen may be helpful to minimise or counteract relapse after orthodontic treatment and to improve patient satisfaction during the typically lengthy post-treatment period.

Highlights • Saliva is an eligible matrix for SARS-CoV-2 molecular detection and IgA measurement. • Saliva collection offers several advantages: safe, non-invasive and self-collection. • Positive molecular testing results were associated with disease duration. • The presence of salivary IgA was associated with pneumonia and CRP values., Aim This study aims to verify whether standardized saliva collection is suitable for SARS-CoV-2 molecular detection and IgA measurement. Methods 43 COVID-19 inpatients and 326 screening subjects underwent naso-pharyngeal (NP)-swab and saliva collection (Salivette). Inpatients also underwent repeated blood collections to evaluate inflammation and organs involvement. In all patients and subjects, SARS-CoV-2 (gene E) rRT-PCR was undertaken in saliva and NP-swabs. Salivary IgA and serum IgA, IgG, IgM were measured on inpatients’ samples. Results NP-swabs and saliva were both SARS-CoV-2 positive in 7 (16%) or both negative in 35 (82%) out of 43 patients successfully included in the study. NP-swabs and saliva results did not perfectly match in one patient (saliva positive, NP-swab negative). Positive molecular results were significantly associated with disease duration (p = 0.0049). 326/326 screening subjects were SARS-CoV-2 negative on both NP-swabs and saliva. Among the 27 saliva samples tested for IgA, 18 were IgA positive. Salivary IgA positivity was associated with pneumonia (p = 0.002) and CRP values (p = 0.0183), not with other clinical and molecular data, or with serum immunoglubulins. Conclusions A standardized saliva collection can be adopted to detect SARS-CoV-2 infection in alternative to NP-swabs. Preliminary data on salivary IgA support the use of saliva also for patient monitoring.

Introduction: There is increasing evidence that intermediate and long-term bowel dysfunction may occur as a consequence of radical surgery for rectal deep endometriosis (DE). Typical symptoms include constipation, feeling of incomplete evacuation, clustering of stools, and urgency. This is described in the colorectal surgical literature as low anterior resection syndrome (LARS). Within this, several studies suggested that differences regarding functional outcomes could be favourable to more conservative surgical approaches, that is, excision of endometriotic tissue with preservation of the luminal structure of the rectal wall when compared with classical segmental resection techniques for DE, especially when performed for low DE. Material and methods: A total of 211 patients undergoing rectal surgery for low DE (≤7 cm from the anal verge) in three different tertiary referral centers between October 2009 and December 2018 were retrospectively reviewed regarding major complications and LARS. From the 211 eligible patients, six women were excluded because of loss to follow up. Finally, a total number of 205 patients were enrolled for the statistical analysis; 139 with nerve- and vessel-sparing segmental resection (NVSSR) and 66 operated for laparoscopic-transanal disk excision (LTADE) were included. Gastrointestinal functional outcomes of the two procedures were compared using the validated LARS questionnaire. The median follow-up time was 46 ± 11 months. As a secondary outcome, the surgical sequelae were examined. Results: We found no statistically significant difference between the incidence of LARS (31.7% and 37.9%, respectively) among patients operated by LTADE when compared with NVSSR (P =.4). The occurrence of LARS was positively associated with the use of protective ileostomy or colostomy (P =.02). A higher rate of severe complications was observed in women undergoing LTADE (19.7%) when compared with patients with NVSSR (9.0%, P =.029). Conclusions: LARS is not more frequent after NVSSR when compared with a more conservative approach such as LTADE in patients undergoing rectal surgery for low DE. To confirm our findings prospective studies are required. © 2020 Nordic Federation of Societies of Obstetrics and Gynecology (NFOG). Published by John Wiley & Sons Ltd

Kawasaki disease (KD) is one of the most common vasculitides of childhood and the main cause of acquired heart disease in developed countries. Intravenous immunoglobulin (IVIG) in association with aspirin represents the main treatment for KD. However, 10–20% of patients fail to respond to standard treatment and have an increased risk of cardiac complications. There is currently no accepted protocol for treatment of resistant cases. Several authors highlighted the role of interleukin-1 (IL-1) as a mediator of inflammation in KD and suggested the possibility of using IL-1 or its receptor as a target of therapy. The use of IL-1 inhibitors in patients with KD has been reported in the scientific literature, but data are largely limited to individual case reports and small case series. We summarized the scientific literature related to the use of anakinra, analyzing preclinical and clinical data. Thirty-eight patients have been described so far, most of them with KD-related complications. Twenty-two were described in case reports and case series, while 16 were patients from the completed KAWAKINRA phase IIa study. Almost all patients received clinical benefit, and no relevant side effects were noted. Based on this evidence, in our opinion, anakinra may be considered as an option after the failure of the first IVIG infusion, especially in patients with coronary involvement.

Background: Vegetarian and vegan weaning have increasing popularity among parents and families. However, if not correctly managed, they may lead to wrong feeding regimens, causing severe nutritional deficiencies requiring specific nutritional support or even the need for hospitalization. Aim: To assess the prevalence of vegetarian and vegan weaning among Italian families and to provide an up-to-date narrative review of supporting evidence. Materials and methods: We investigated 360 Italian families using a 40-item questionnaire. The narrative review was conducted searching scientific databases for articles reporting on vegetarian and vegan weaning. Results: 8.6% of mothers follow an alternative feeding regimen and 9.2% of infants were weaned according to a vegetarian or vegan diet. The breastfeeding duration was longer in vegetarian/vegan infants (15.8 vs. 9.7 months; p < 0.0001). Almost half of parents (45.2%) claim that their pediatrician was unable to provide sufficient information and adequate indications regarding unconventional weaning and 77.4% of parents reported the pediatrician’s resistance towards alternative weaning methods. Nine studies were suitable for the review process. The vast majority of authors agree on the fact that vegetarian and vegan weaning may cause severe nutritional deficiencies, whose detrimental effects are particularly significant in the early stages of life. Discussion and conclusion: Our results show that alternative weaning methods are followed by a significant number of families; in half of the cases, the family pediatrician was not perceived as an appropriate guide in this delicate process. To date, consistent findings to support both the safety and feasibility of alternative weaning methods are still lacking. Since the risk of nutritional deficiencies in the early stages of life is high, pediatricians have a pivotal role in guiding parents and advising them on the most appropriate and complete diet regimen during childhood. Efforts should be made to enhance nutritional understanding among pediatricians as an unsupervised vegetarian or vegan diet can cause severe nutritional deficiencies with possible detrimental long-term effects.

To solve conservation and planning challenges in the marine environment, researchers are increasingly developing geospatial tools to address impacts of anthropogenic activities on marine biodiversity. The paper presents a comprehensive set of built-in geospatial webtools to support Maritime Spatial Planning (MSP) and environmental management objectives implemented into the Tools4MSP interoperable GeoPlatform. The webtools include cumulative effects assessment (CEA), maritime use conflict (MUC) analysis, MSFD pressure-driven CEA and a CEA-based marine ecosystem service threat analysis (MES-Threat) . The tools are tested for the Northern Adriatic (NA) Sea, one of the most industrialized sea areas of Europe using a case study driven modelling strategy. Overall results show that coastal areas within 0-9 nm in the Gulf of Trieste, Grado-Marano and Venice lagoon and Po Delta outlet are subjected to intense cumulative effects and high sea use conflicts mainly from port activities, fishery, coastal and maritime tourism and maritime shipping. Linking MES into CEA provided novel information on locally threatened high MES supporting and provisioning habitats such Cymodocea beds and infralittoral fine sands, threats to cultural MES are most pronounced in coastal areas. Results are discussed for their geospatial relevance for regional planning, resource management and their applicability within MSP and environmental assessment., Funding: This work was supported by: the Flagship Project RITMARE - Italian Research for the Sea, coordinated by the Italian National Research Council and funded by the Italian Ministry of Education, University and Research within the National Research Program 2012–2016.

Background The authors evaluated the morphology and symmetry of the temporomandibular joint in participants with normodivergent and hyperdivergent skeletal class I, II, and III patterns. Methods A total of 80 participants were divided into 4 groups on the basis of their sagittal and vertical skeletal patterns. Cone-beam computed tomographic images were used to evaluate the condyle-fossa relationship and the morphology and symmetry of the mandibular condyle. One-way analysis of variance and Tukey post hoc tests were used to compare the mean values among the different groups. Results Participants with class II hyperdivergent patterns had the smallest anteroposterior (mean [standard deviation {SD}], 4.4 [1.6] millimeters) and mediolateral (10.5 [3.0] mm) condylar process widths among all 4 groups. The mean (SD) axial condylar angle was flatter in patients with class III hyperdivergent patterns (19.8° [5.1°]) compared with the other groups. The mean (SD) anteroposterior differences of the condylar processes (2.9 [1.4] mm) in patients with class III hyperdivergent patterns were the greatest in all 4 groups. Conclusion Participants in the group with class II hyperdivergent patterns have a smaller and narrower condyle compared with the other groups measured. Asymmetry was found among all groups, with participants with skeletal class III patterns having the most asymmetry. The most common condylar morphology in all groups examined was convex. Practical Implications These results support the concept that morphology and symmetry of the temporomandibular joint varies in different skeletal patterns, presumably as an adaptive response to functional demands.

The paper presents an investigation about the combination of multispectral and 3D imaging aiming at the analysis of the condition and preservation of an ancient vase. Visible-reflected (VIS) and -induced luminescence (UVL) images are mapped to 3D models produced with image- and range-based 3D modelling techniques. The case study is an Attic vase, part of the pottery collection of the Landesmuseum Rudolfinum (Carinthia, Austria) and temporarily stored in the Institute of Archaeology of the University of Graz, Austria. The aim of this study is to exploit the added-value provided by mapping multispectral imaging onto 3D geometry for a comprehensive knowledge of the condition of a restored Cultural Heritage (CH) item., International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences, XLII-2, ISSN:1682-1750, ISSN:2194-9034, ISSN:1682-1777

// Nicola Coppola 1 , Giorgio de Stefano 2 , Marta Panella 3 , Lorenzo Onorato 1 , Valentina Iodice 2 , Carmine Minichini 1 , Nicola Mosca 3 , Luisa Desiato 3 , Nunzia Farella 2 , Mario Starace 1 , Giulia Liorre 2 , Nicoletta Potenza 3 , Evangelista Sagnelli 1 , Aniello Russo 3 1 Department of Mental Health and Public Medicine, Second University of Naples, Naples, Italy 2 IX Interventional Ultrasound Unit for Infectious Diseases, AORN dei Colli, P.O. Cotugno, Naples, Italy 3 Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Naples, Italy Correspondence to: Aniello Russo, email: aniello.russo@unina2.it Keywords: microRNA, gene regulation, cell proliferation, tumor, hepatocellular carcinoma Received: November 08, 2016 Accepted: February 07, 2017 Published: February 28, 2017 ABSTRACT Human microRNA-125a-5p (miR-125a) is expressed in most tissues where it downregulates the expression of membrane receptors or intracellular transductors of mitogenic signals, thus limiting cell proliferation. Expression of this miRNA generally increases with cell differentiation whereas it is downregulated in several types of tumors, such as breast, lung, ovarian, gastric, colon, and cervical cancers, neuroblastoma, medulloblastoma, glioblastoma, and retinoblastoma. In this study, we focused on hepatocellular carcinoma and used real-time quantitative PCR to measure miR-125a expression in 55 tumor biopsies and in matched adjacent non-tumor liver tissues. This analysis showed a downregulation of miR-125a in 80 % of patients, with a mean decrease of 4.7-fold. Comparison of miRNA downregulation with clinicopathological parameters of patients didn’t yield significant correlations except for serum bilirubin. We then evaluated the expression of known targets of miR-125a and found that sirtuin-7, matrix metalloproteinase-11, and c-Raf were up-regulated in tumor tissue by 2.2-, 3-, and 1.7-fold, respectively. Overall, these data support a tumor suppressor role for miR-125a and encourage further studies aimed at the comprehension of the molecular mechanisms governing its expression, eventually leading to treatments to restore its expression in tumor cells.

Interproximal reduction (IPR) is the deliberate removal of part of the dental enamel from the interproximal contact areas, which decreases the mesiodistal width of a tooth. This enamel may be removed for various reasons, but most commonly to create space during orthodontic treatment or to correct tooth-size discrepancies. Several authors have also encouraged its use as a method by which post-orthodontic stability might be enhanced, particularly in the lower anterior region. With the increased use of removable aligners for orthodontic treatment in which non-extraction therapy is often advocated, the use of IPR becomes a valuable tool to relieve crowding without over-expanding the dental arches. It is possible that inaccurate IPR could result in the over-reduction of enamel, the creation of ledges and notches in the proximal surfaces, increased tooth sensitivity or damage to the surrounding soft tissues. However, carefully conducted IPR performed within the recommended guidelines may be used as a safe method to gain space for the relief of crowding, to correct tooth-size discrepancies and to improve aesthetics and long-term stability in selected orthodontic patients.

Background Timely referrals of appropriately screened cases are essential for the effective delivery of orthodontic treatment. Dental therapists are intimately involved with the orthodontic screening process in New Zealand, given that they are the primary oral health providers for child and adolescent patients. Objectives (1) To investigate New Zealand dental therapists’ orthodontic screening and referral practices; and, (2) to quantify the perceived need for supplementary orthodontic resources by New Zealand dental therapists. Methods An online questionnaire was distributed via email to 659 actively practising dental therapists in New Zealand. Participants answered questions related to their socio-demographic characteristics, orthodontic screening and referral practices, and further orthodontic education. Results All surveyed dental therapists viewed orthodontics as an important treatment priority. Most (64.6%, N = 148) agreed that the orthodontic screening process should be a joint undertaking between general dental practitioners and dental therapists. Most practitioners (63.3%, N = 145) had access to (and used) an orthodontic screening guideline. While almost all dental therapists (98.7%, N = 226) were confident in assessing cases that were suitable for orthodontic referral, 63.8% felt that they could gain from further education, and virtually all (99.6%, N = 228) believed that continuing professional development (CPD) courses in orthodontics would be beneficial. Over three-quarters were in favour of a standardised national guideline for orthodontic screening, while the remainder were either satisfied with their current guidelines (15.3%, N = 35) or believed that such guidelines were unnecessary (7.0%, N = 16). Several patterns were observed by therapist characteristics, particularly related to working sector (private or public) and length of professional experience. Conclusions There were differences in the orthodontic screening and referral practices of dental therapists in New Zealand. Dental therapists were receptive to the idea of standardised guidelines for orthodontic screening and there was a perceived need for CPD courses in orthodontics.

Summary The relationship between facial morphology and jaw function remains controversial. The purpose of this study was to investigate differences in self-reported oral behaviour habits between individuals with normodivergent and hyperdivergent facial types. Some 80 cases and controls were individually matched on age, sex ethnicity and treatment stage. The participants were recruited from an orthodontic clinic, and included both adolescents and adults. Habitual oral activity was assessed using the Oral Behaviour Checklist (OBC) based on their experiences in the past 4 weeks. Univariate and bivariate analyses were performed. The sample had a mean age of 17·2 years (SD = 4·6; range = 12–49 years), and was predominantly female (65·0%) and of New Zealand European origin (91·3%). The prevalence of reporting one or more frequently performed habitual muscular behaviour in either study group was over 85% (P > 0·05). There was no difference in total OBC score between the hyperdivergent (25·6; SD: 9·0) and normodivergent group (25·3; SD: 9·9). Moreover, there was no difference in the prevalence of either nocturnal or daytime oral behaviours between the two groups. While this study did not include any objective measures of functional or habitual activity, we found no differences in self-reported oral behaviour habits between normodivergent and hyperdivergent individuals. The findings do not support an association between vertical facial form and habitual muscular activity.

Introduction The purpose of this study was to morphometrically investigate the growth pattern of the adenoids in growing subjects with hyperdivergent and hypodivergent vertical craniofacial features. Methods In this retrospective study, we used a longitudinal sample of lateral cephalometric radiographs of 28 hyperdivergent and 30 hypodivergent subjects from 4 to 13 years of age. The radiographs were obtained from the American Association of Orthodontists Foundation Craniofacial Growth Legacy Collection. Measurements were made using digital tracings of the lateral cephalograms and point distribution models. Mixed-model analyses were used for statistical analysis. Results The mean distance between the sphenoid bone and the posterior nasal spine increased up to 5.3 mm over a 9-year span (95% CI, 4.1-6.5 mm; P