Objective: To illustrate the process of developing and sustaining an academic-public health partnership for behavioral health integration through an expansion of the Aligning Systems for Health (ASfH) framework. Study Setting: Practice-informed primary data (2017-2023) from the Holistic Opportunity Program for Everyone (HOPE) Initiative based in Charlotte, NC. Study Design: The unit of analysis in this descriptive case study is interorganizational, specifically focusing on an academic-public health relationship. We illustrate the partnership process across the ASfH four core areas, including key challenges and insights. Data Collection: Utilized a Critical Moments Reflection methodology and review of HOPE program data. Principal Findings: (1) Formal partnership structures and processes are essential to monitoring the four ASfH core components for on-going system alignment. (2) Aligning systems for health principally involves two ecologies: (i) the health program and (ii) the partnership. The vitality and sustainability of both ecologies require continuous attention and resource investment. (3) Relationships rest at the heart of aligning systems. (4) With comparative advantages in research methods, the academic sector is especially poised to collaborate with healthcare systems and human service organizations to study, develop, implement, and scale evidence-based health interventions. Conclusions: The academic sector shares overlapping purposes with the public health, healthcare, and social services sectors while providing complementary value. It is a critical sectoral partner in advancing population health and health equity. KEYWORDS academic-public health partnership, aligning systems for health, case study, cross-sector partnership, health equity, integrated care What is known on this topic * Cross-sector partnerships are critical to promoting community health and health equity. However, the pathways to effective and sustainable cross-sector partnerships are less well understood. * Aligning Systems for Health is an emergent, prominent theoretical framework for building cross-sector alignment that requires field-testing and refinement. * While the benefits of academic-public health partnerships are well-recognized, descriptive studies about how to effectively establish and sustain this type of cross-sector partnership remain limited. What this study adds * This study expands the Aligning Systems for Health theoretical framework to include the academic sector and demonstrates the opportunities and value associated with integrating this sector. * The article illuminates the process and pitfalls of academic-public health partnership formation, growth, and maintenance; and lends generalizable practice insights for cross-sector partnerships more broadly., 1 | INTRODUCTION Cross-sector partnerships are essential to improving health equity and population health outcomes. (1,2) Defined as a formal alliance between two or more organizations representing different sectors of [...]