5 results on '"Wen, Xiaojian"'
Search Results
2. Differences Between Microbial Communities of Pinus Species Having Differing Level of Resistance to the Pine Wood Nematode
- Author
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Xuan Wang, Wei Zhang, Yongxia Li, Xingyao Zhang, Wen Xiaojian, Dongzhen Li, Yuqian Feng, Zhenkai Liu, Ningning Sun, and Pengfei Wei
- Subjects
Geosmithia ,animal structures ,Bacteria ,Nematoda ,Ecology ,biology ,Firmicutes ,Microbiota ,fungi ,food and beverages ,Soil Science ,Bursaphelenchus xylophilus ,Pinus ,biology.organism_classification ,complex mixtures ,Microbial ecology ,Eurotiomycetes ,Botany ,Animals ,Proteobacteria ,Introduced Species ,Ecology, Evolution, Behavior and Systematics ,Plant Diseases ,Acidobacteria ,Cladosporium - Abstract
The pine wood nematode (PWN), Bursaphelenchus xylophilus, is a destructive invasive species that exerts devastating effects on most native pines in invaded regions, while many of the non-native pines have resistance to PWN. Recently, increasingly more research is focused on how microbial communities can improve host resistance against pathogens. However, the relationship between the microbial community structures and varying levels of pathogen resistance observed in different pine tree species remains unclear. Here, the bacterial and fungal communities of introduced resistant pines Pinus elliottii, P. caribaea, and P. taeda and native susceptible pines healthy and wilted P. massoniana infected by PWN were analyzed. The results showed that 6057 bacterial and 3931 fungal OTUs were annotated. The pine samples shared 944 bacterial OTUs primarily in the phyla Proteobacteria, Acidobacteria, Firmicutes, Bacteroidetes, and Chloroflexi and 111 fungal OTUs primarily in phyla Ascomycota and Basidiomycota, though different pines had unique OTUs. There were significant differences in microbial community diversity between different pines, especially between the bacterial communities of resistant and susceptible pines, and fungal communities between healthy pines (resistant pines included) and the wilted P. massoniana. Resistant pines had a greater abundance of bacteria in the genera Acidothermus (class unidentified_Actinobacteria) and Prevotellaceae (class Alphaproteobacteria), but a lower abundance of Erwinia (class Gammaproteobacteria). Healthy pines had a higher fungal abundance of Cladosporium (class Dothideomycetes) and class Eurotiomycetes, but a lower abundance of Graphilbum, Sporothrix, Geosmithia (class Sordariomycetes), and Cryptoporus (classes Agaricomycetes and Saccharomycetes). These differences in microbial abundance between resistant and healthy pines might be associated with pathogen resistance of the pines, and the results of this study contribute to the studies exploring microbial-based control of PWN.
- Published
- 2021
3. Pinewood Nematode Alters the Endophytic and Rhizospheric Microbial Communities of Pinus massoniana
- Author
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Wei Zhang, Xuan Wang, Dongzhen Li, Xingyao Zhang, Yuqian Feng, Wen Xiaojian, Yongxia Li, and Zhenkai Liu
- Subjects
0301 basic medicine ,China ,animal structures ,Burkholderiaceae ,Pinus massoniana ,Nematoda ,030106 microbiology ,Soil Science ,Bursaphelenchus xylophilus ,complex mixtures ,03 medical and health sciences ,Microbial ecology ,Botany ,Animals ,Pestalotiopsis ,Ecology, Evolution, Behavior and Systematics ,Rhizosphere ,Bacteria ,Ecology ,biology ,Microbiota ,fungi ,food and beverages ,Pinus ,biology.organism_classification ,030104 developmental biology ,Xylophilus ,Trichoderma - Abstract
Pinewood nematode, Bursaphelenchus xylophilus, is one of the greatest threats to pine trees and is spreading all over the world. During the nematode's pathogenesis, plant microorganisms play important roles. However, many microbial communities, such as that in Pinus massoniana, a major host of B. xylophilus that is widely distributed in China, are not well studied, especially the fungal communities. Here, the endophytic and rhizospheric bacterial and fungal communities associated with healthy and B. xylophilus-infected P. massoniana were analyzed. The results showed that 7639 bacterial and 3108 fungal OTUs were annotated from samples of P. massoniana, the rhizosphere, and B. xylophilus. There were significant diversity differences of endophytic microbes between healthy and infected P. massoniana. The abundances of endophytic bacteria Paenibacillus, unidentified_Burkholderiaceae, Serratia, Erwinia, and Pseudoxanthomonas and fungi Penicillifer, Zygoascus, Kirschsteiniothelia, Cyberlindnera, and Sporothrix in infected pines were greater than those in healthy pines, suggesting an association of particular microbial abundances with the pathogenesis of B. xylophilus in pines. Meanwhile, the abundances of microbes of unidentified_Burkholderiaceae, Saitozyma, and Pestalotiopsis were greater and Acidothermus and Trichoderma were lower in the rhizosphere under infected pines than those under healthy pines and the differences might be caused by B. xylophilus-induced weakening of the health of pines. Our study explored the endophytic and rhizospheric microbial community changes potentially caused by B. xylophilus infection of pines.
- Published
- 2020
4. Identification of the Extracellular Nuclease Influencing Soaking RNA Interference Efficiency in Bursaphelenchus xylophilus.
- Author
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Wang, Ruijiong, Li, Yongxia, Li, Dongzhen, Zhang, Wei, Wang, Xuan, Wen, Xiaojian, Liu, Zhenkai, Feng, Yuqian, and Zhang, Xingyao
- Subjects
PINEWOOD nematode ,RNA ,GENE expression ,DOUBLE-stranded RNA ,GENE silencing ,NUCLEASES - Abstract
RNA interference (RNAi) efficiency dramatically varies among different nematodes, which impacts research on their gene function and pest control. Bursaphelenchus xylophilus is a pine wood nematode in which RNAi-mediated gene silencing has unstable interference efficiency through soaking in dsRNA solutions, the factors of which remain unknown. Using agarose gel electrophoresis, we found that dsRNA can be degraded by nematode secretions in the soaking system which is responsible for the low RNAi efficiency. Based on the previously published genome and secretome data of B. xylophilus, 154 nucleases were screened including 11 extracellular nucleases which are potential factors reducing RNAi efficacy. To confirm the function of nucleases in RNAi efficiency, eight extracellular nuclease genes (BxyNuc1-8) were cloned in the genome. BxyNuc4, BxyNuc6 and BxyNuc7 can be upregulated in response to dsGFP, considered as the major nuclease performing dsRNA degradation. After soaking with the dsRNA of nucleases BxyNuc4/BxyNuc6/BxyNuc7 and Pat10 gene (ineffective in RNAi) simultaneously for 24 h, the expression of Pat10 gene decreased by 23.25%, 26.05% and 11.29%, respectively. With soaking for 36 h, the expression of Pat10 gene decreased by 43.25% and 33.25% in dsBxyNuc6+dsPat10 and dsBxyNuc7+dsPat10 groups, respectively. However, without dsPat10, dsBxyNuc7 alone could cause downregulation of Pat10 gene expression, while dsBxyNuc6 could not disturb this gene. In conclusion, the nuclease BxyNuc6 might be a major barrier to the RNAi efficiency in B. xylophilus. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
5. Transcriptome Analysis of Dauer Moulting of a Plant Parasitic Nematode, Bursaphelenchus xylophilus Promoted by Pine Volatile β-Pinene.
- Author
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Zhang, Wei, Li, Yongxia, Liu, Zhenkai, Li, Dongzhen, Wen, Xiaojian, Feng, Yuqian, Wang, Xuan, and Zhang, Xingyao
- Subjects
PINEWOOD nematode ,MOLTING ,CONIFER wilt ,PARASITIC plants ,CYSTATHIONINE gamma-lyase ,LIFE cycles (Biology) ,METABOLIC regulation - Abstract
Pinewood nematode, Bursaphelenchus xylophilus, a pine-parasitic nematode, poses a serious threat to pine trees globally, causing pine wilt disease. When dispersal-stage juvenile 4 (dauer, J
IV , a durable stage) of B. xylophilus enters the new pine, it transforms into a propagative adult (dauer moulting) and reproduces quickly. Our previous studies have found that pine-volatile β-pinene promotes dauer moulting of B. xylophilus; however, this mechanism is not clear. Here, this study is attempting to unravel the molecular process underlying dauer moulting of B. xylophilus through signal chemical tests and transcriptome analysis. The results showed that β-pinene could promote dauer moulting of B. xylophilus, while other common dauer moulting signals, such as dafachronic acid (DA), part of the TGF/insulin signal pathway, were inoperative. Moreover, the JIV soaked in 1% β-pinene for only 6 h could transform into adults at a significant rate. Therefore, the transcriptomes of JIV soaked in 1% β-pinene for 6 h were sequenced. It was found that 15,556 genes were expressed; however, only 156 genes were expressed differentially and enriched in the metabolism of xenobiotics, peroxisome, fatty acid metabolism, and carbon metabolism, indicating that energy metabolism was active at the early stage of dauer moulting. With a stricter parameter, the number of differential genes fell to 19, including 4 sterol hydroxylase, 5 dehydrogenase, 2 glucuronosyltransferase, 5 nuclear-related factor, 1 calcium-binding protein, 1 nitrogen metabolic regulation protein, and 1 cystathionine gamma-lyase. These results indicated that dauer moulting of B. xylophilus into adults might not be regulated by the TGF-β/insulin signal pathway but by another new signal pathway related to the 19 differential genes which need more exploration. Our results contribute to the understanding of the molecular mechanisms behind dauer moulting and may be useful in reducing pine wilt disease by suppressing this moulting to cut the life cycle of B. xylophilus. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
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