1. [The therapeutic use of diucifon-activated immunocytes in adoptive transfer in mice with burn trauma complicated by Pseudomonas aeruginosa infection].
- Author
-
Prozorovskiĭ NS, Radkevich SA, Radchenkov VP, Moroz AF, Leskov VP, and Gushchin IS
- Subjects
- Animals, Burns complications, Burns immunology, Burns mortality, Drug Combinations, Drug Evaluation, Preclinical, Lymphocyte Activation immunology, Lymphocytes drug effects, Lymphocytes immunology, Mice, Mice, Inbred CBA, Pseudomonas Infections etiology, Pseudomonas Infections immunology, Pseudomonas Infections mortality, Spleen immunology, Uracil therapeutic use, Adjuvants, Immunologic therapeutic use, Burns therapy, Immunotherapy, Adoptive methods, Lymphocyte Activation drug effects, Pseudomonas Infections therapy, Sulfones therapeutic use, Uracil analogs & derivatives
- Abstract
The effectiveness of the therapeutic use of diucyphone-stimulated syngeneic spleen cells was studied in mice with a burn trauma complicated by P. aeruginosa infection. For this purpose CBA mice were subjected to a burn of degree IIIa, covering 30% of their body surface, the wound was infected with P. aeruginosa strain, and 24 hours later spleen cells were taken from some of these animals. The spleen cells, incubated for 3 hours with or without diucyphone at a concentration of 10-100 micrograms/ml, were washed and reinfused intravenously to the remaining animals. The injection of diucyphone-treated spleen cells was shown to greatly increase the survival rate of the animals, to accelerate healing processes and to enhance the proliferative response of spleen cells to mitogens (phytohemagglutinin, concanavalin A) and interleukin-2. A conclusion was made on the efficacy of the therapeutic use of diucyphone-activated immunocompetent cells in cases of immunodeficiency induced by burn infection.
- Published
- 1991