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The article presents a study regarding the effects of amino acids and vitamins on the in vitro development of buffalo embryos derived from in vitro fertilization (IVF) in China. The study concluded that nonessential amino acids experienced an increase in the rate of IVF embryos. It also mentions the effects in both high and low concentration amino acids wherein low concentration was beneficial to the development of buffalo embryos but high concentration was detrimental to the development.

The article presents a study which determines the effect of the cytochalasin B (CB) on the development of buffalo somatic cell nuclear transfer (NT) embryos. The result showed that addition of CB to electrofusion medium has no profound effect on the development of buffalo NT embryos. Additionally, there was no beneficial effect on its development, when treated buffalo NT embryos with CB after activation, but treated them with CB before activation can improve their development.

The article presents a study which examines the effects of somatic cell serial nuclear transfer (SCSNT) on the somatic cell nuclear transfer (SCNT) in buffalo. Researchers perform SCSNT using the fetal cells of buffalo as the donor cells and compare the fusion rate and cleavage rate of SCSNT with those of SCNT. Results demonstrate the feasibility of buffalo SCSNT and the ability of buffalo SCNT embryos to be recloned without the elimination of their development ability.