1. Contrasting roles of macrophages and dendritic cells in controlling initial pulmonary Brucella infection.
- Author
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Archambaud C, Salcedo SP, Lelouard H, Devilard E, de Bovis B, Van Rooijen N, Gorvel JP, and Malissen B
- Subjects
- Animals, Bacterial Load, Brucella abortus growth & development, Brucella abortus pathogenicity, Brucellosis microbiology, Brucellosis transmission, Cell Count, Cell Movement immunology, Dendritic Cells immunology, Dendritic Cells microbiology, Dendritic Cells pathology, Immunity, Innate, Lung immunology, Lung microbiology, Lung pathology, Lymph Nodes microbiology, Macrophages, Alveolar immunology, Macrophages, Alveolar microbiology, Macrophages, Alveolar pathology, Mice, Mice, Inbred C57BL, Nitric Oxide Synthase Type II genetics, Nitric Oxide Synthase Type II metabolism, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha metabolism, Brucella abortus immunology, Brucellosis immunology, Dendritic Cells metabolism, Lymph Nodes immunology, Macrophages, Alveolar metabolism
- Abstract
Control of pulmonary pathogens constitutes a challenging task as successful immune responses need to be mounted without damaging the lung parenchyma. Using immunofluorescence microscopy and flow cytometry, we analyzed in the mouse the initial innate immune response that follows intranasal inoculation of Brucella abortus. Bacteria were absent from parenchymal dendritic cells (DC) but present in alveolar macrophages in which they replicated. When the number of alveolar macrophages was reduced prior to Brucella infection, small numbers of pulmonary DC were infected and a massive recruitment of TNF-α- and iNOS-producing DC ensued. Coincidentally, Brucella disseminated to the lung-draining mediastinal lymph nodes (LN) where they replicated in both migratory DC and migratory alveolar macrophages. Together, these results demonstrate that alveolar macrophages are critical regulators of the initial innate immune response against Brucella within the lungs and show that pulmonary DC and alveolar macrophages play rather distinct roles in the control of microbial burden., (Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2010
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