1. Long chain fatty acid coenzyme A ligase (FACL) regulates triazophos-induced stimulation of reproduction in the small brown planthopper (SBPH), Laodelphax striatellus (Fallen)
- Author
-
Jin-Cai Wu, Xian Zhang, Lin-Quan Ge, Bing Xu, Jun Ding, and Guo-Qing Yang
- Subjects
Glycerol ,Proteomics ,0106 biological sciences ,0301 basic medicine ,Integrated pest management ,Insecticides ,Long-chain-fatty-acid-coenzyme A ligase ,Health, Toxicology and Mutagenesis ,media_common.quotation_subject ,Down-Regulation ,Gene Expression ,Stimulation ,Virus diseases ,Biology ,Real-Time Polymerase Chain Reaction ,Zea mays ,01 natural sciences ,Virus ,Microbiology ,Hemiptera ,03 medical and health sciences ,Coenzyme A Ligases ,Animals ,Gene Silencing ,Gene ,Plant Diseases ,media_common ,Reproduction ,Body Weight ,Organothiophosphates ,food and beverages ,Oryza ,General Medicine ,Triazoles ,biology.organism_classification ,Up-Regulation ,010602 entomology ,030104 developmental biology ,Female ,Brown planthopper ,Agronomy and Crop Science - Abstract
The small brown planthopper (SBPH), Laodelphax striatellus (Fallen) is a major pest insect of rice, wheat, and maize in China and other countries. SBPH not only damage rice plants through sucking plant sap, but also transmits rice virus diseases, for example, striped virus disease (RSV), black streaked dwarf, and maize rough disease virus. Therefore, understanding of pesticide-induced stimulation of reproduction in SBPH is of great significance for the pest management. Our previous study discovered that triazophos (TZP) increased reproduction of SBPH. But the molecular mechanisms are unclear. Here, by using proteomic analysis, we screened and cloned the gene of long chain fatty acid coenzyme A ligase ( FACL ), and silenced FACL to examine influences of TZP on reproduction and glycerin content in SBPH females. In TZP-treated females vs control females, there were 41 differential proteins in 18 pathways related to reproduction, of which 8 were up-regulated and 33 were down-regulated. TZP + dsFACL eliminated TZP-induced stimulation of reproduction of SBPH females (↓about 73.92%) and decreased glycerin content and body weight (↓about 19.93% and 13.62%). TZP + dsFACL treatment led to reduced expression of FACL (↓about 61.88%). FACL is a key gene of TZP-induced increase of reproduction of SBPH.
- Published
- 2018