1. Corrigendum to 'Special Staining of the Liquid-Based Cytopathology Test in Bronchoalveolar Lavage Fluid for Diagnosis of Invasive Pulmonary Aspergillosis with Nonneutropenic Patients'
- Author
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Yiming Yu, Zhongbo Chen, Lei Shao, Xianfa Xu, Deng Pan, Zaichun Deng, Qidong Zhuang, Lin Zheng, and Yue Hu
- Subjects
Pulmonary and Respiratory Medicine ,Male ,Microbiological Techniques ,medicine.medical_specialty ,China ,Tuberculosis ,Grocott's methenamine silver stain ,Gastroenterology ,Sensitivity and Specificity ,Diseases of the respiratory system ,03 medical and health sciences ,0302 clinical medicine ,Bronchoscopy ,Predictive Value of Tests ,Internal medicine ,medicine ,Humans ,030212 general & internal medicine ,Retrospective Studies ,Invasive Pulmonary Aspergillosis ,RC705-779 ,medicine.diagnostic_test ,Staining and Labeling ,business.industry ,Liquid Biopsy ,Invasive pulmonary aspergillosis ,Middle Aged ,medicine.disease ,respiratory tract diseases ,Staining ,Bronchoalveolar lavage ,030228 respiratory system ,Cytopathology ,Predictive value of tests ,Female ,business ,Corrigendum ,Bronchoalveolar Lavage Fluid - Abstract
In recent years, various biomarkers have been gradually applied on bronchoalveolar lavage (BAL) fluid for the diagnosis of invasive pulmonary aspergillosis (IPA). The objective of this study is to assess the value of the liquid-based cytopathology test (LCT) for improving the identification of IPA in BAL fluid from possible IPA patients, following special staining with periodic acid-Schiff staining (PAS) or Grocott's methenamine silver (GMS). A total of 47 consecutive possible IPA patients who underwent bronchoscopy with BAL fluid from January 2017 to December 2018 were included. 45 people had a pair of BAL fluid specimens and 2 patients had two BAL fluid specimens. The 49 pairs of BAL fluid specimens were processed for culture, tuberculosis acid fast staining smear, direct microbial smear, and LCT with special staining (PAS and GMS), respectively. Then, we compared the sensitivity and specificity of PAS and GMS in BAL fluid in high-risk patients. Among 47 possible IPA patients, 25 patients had proven/probable IPA, and 11 patients had other invasive fungal diseases. The sensitivity of GMS was higher than that of PAS (92.11% versus 81.58%; P = 0.175). The specificity of GMS was 81.82%, which was higher than that of PAS (81.82% versus 72.73%; P = 0.611). The negative predictive value (NPV) for PAS and GMS were 53.33% and 75.00%, respectively. The positive predictive value (PPV) for PAS and GMS were 91.18% and 94.59%, respectively. This study showed that special staining of LCT in BAL fluid may be a novel method for the diagnosis of IPA, and the GMS of LCT had higher sensitivity and specificity, which was superior to PAS.
- Published
- 2020