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This study investigated the effect of Phyllanthus emblica leaves and branches mixture (PE) on growth performance, oxidative status and intestinal characteristics in broilers, as well as the in vitro antioxidant potential of PE extract (PEE). As the result of in vitro evaluation, the PEE had a total phenolic compound content of 95.5 ± 2.75 mg of Gallic acid equivalent/g dry weight (DW), and a total flavonoid content of 3.04 ± 0.06 mg of quercetin equivalent/g DW. The PEE exhibited significant antioxidant activity through various mechanisms, including chelation of ferrous iron, reducing power, scavenging 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals, inhibition of liposome peroxidation and trolox equivalent antioxidant capacity (TEAC). A total of 320 Ross 308 broilers were randomly allocated into four groups: control, basal diet supplemented with 0.5%, 1%, 2% PE. Each group was fed for 35 d. The results showed that the group fed with 0.5% PE had significantly better weight gain, and improved feed conversion ratio (FCR) compared to the control group. Moreover, each PE group significantly reduced coliform and Clostridium perfringens in the ileum and caecum. Additionally, PE group with 0.5% concentration had significantly higher villus height in the jejunum compared to the other group. Furthermore, compared to the control group, each PE group had significantly lower levels of malondialdehyde (MDA) and higher levels of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity in the serum. In the peripheral blood mononuclear cells (PBMCs) of chickens, each PE group showed significantly higher mRNA levels of antioxidant-regulated genes, including Nrf2, GST, GCLC, SOD1 and HO-1. In contrast, the expression levels of NOX1 and ROMO1 genes were significantly lower. In conclusion, the inclusion of 0.5% PE in the basal diet could have a positive impact on the growth performance of broilers by modulating intestinal characteristics and oxidative status. Phyllanthus emblica affects the growth performance, oxidative status and intestinal characteristics in broilers. The 0.5% PE group had significantly better weight gain and improved FCR. The 0.5% PE in the basal diet could potentially enhance growth performance by modulating intestinal characteristics and oxidative status in broilers. [ABSTRACT FROM AUTHOR]

This study was conducted to evaluate the dietary supplementation ofTrichoderma pseudokoningiienzyme powder (EP) and its effect on the growth performance, intestinal morphology and antioxidative status of broilers.In vitroexperiments showed that xylanase and cellulase activities of EP were 487.96 ± 14.14 U/g and 18.55 ± 0.54 U/g, respectively. Total phenolic and ferulic acid content was 4.79 ± 0.35 mg GAE/g DW and 4.38 ± 0.32 μM/g DW, respectively. A total of 240 d-old Ross 308 male broiler chicks were randomly subjected to one of four treatments, namely diets supplemented with dry EP at 0% (control), 0.1%, 0.2% or 0.4% for 35d. Results showed that in the starter phase (1–21 d), the EP supplemented groups had improved body weight, weight gain and FCR compared to the control group. The villus:crypt ratio of both jejunum and ileum in all EP-treated groups was significantly higher than that of the control group. When testing serum antioxidant activity, results showed that 0.2% and 0.4% EP supplementation significantly increased superoxide dismutase activity in both the finisher phase and the overall experimental period. In addition, catalase activity was elevated in all EP supplemented groups in both the finisher phase and throughout the experimental period. 0.1% EP seemed to have no effect during the starter period. Serum malondialdehyde concentration was not affected by EP supplementation. These results suggest that EP is potential feed additive in terms of improving broiler performance and enhancing antioxidative status, while also providing an optimal intestinal environment. [ABSTRACT FROM PUBLISHER]

This study evaluated the effects of 10% solid-state fermentation (SSF) wheat bran (WB) by Bacillus amyloliquefaciens (BA) and Saccharomyces cerevisiae (SC) in broiler diets, on growth performance, intestinal morphology, and intestinal microbiota and serum characteristics of broiler chickens. The results showed that fermented wheat bran with BA or BA and SC reduced NDF and ADF contents in WB material. A total of 240 male broilers (Ross 308) were randomly allocated into four feeding groups: (1). Basal diet with 10% WB (control treatment). (2). Basal diet that replaced WB with fermented WB by BA (FWBA). (3). Fermented WB by SC (FWSC), and (4). Fermented WB by BA and SC (FWBA+SC), each group was fed these particular diets for 35 d. Results demonstrated that replacement of WB with FWBA and FWBA+SC improved feed conversion ratio (d 1 to d 35) compared to the control group. Moreover, the 10% FWBA group significantly enhanced the lactic acid bacteria count in the ileum of 35 d old broilers. Compared to the control group, the 10% FWBA and 10% FWSC groups, showed significantly increased ileal lactic acid levels, and in the 10% FWSC group, the ileum villus height was significantly increased in 35 d old broilers. However, treatments with 10% FWBA and 10% FWBA+SC had a tendency to reduce serum cholesterol. In conclusion, a 10% FWBA replacement in the diet could ameliorate health status in broilers by improving growth performance, modulating intestinal microbiota and increasing lactic acid in the ileum. [ABSTRACT FROM AUTHOR]

This experiment investigated the effects of wheat bran fermented withTrichoderma pseudokoningii(FWB) on growth performance, intestinal morphology and the incidence of non-specific pathological lesions in broilers.In vitroexperimental results showed that cellulase and xylananse activity of fermented wheat bran reached its peak at day 4 and solid-state fermentation (SSF) enhanced the reducing sugar content. In addition, the possibility ofTrichodermaadhering to the broiler’s crop epitheliums was also discovered. A total of 180 day-oldRoss308 male broilers were randomly distributed into one of the three dietary groups until 35 d of age: basal diet (control), 10% of basal diet replaced with wheat bran (10% WB) or 10% of diet replaced with fermented wheat bran (10% FWB). Results showed that 10% FWB group had lower feed consumption than the others, but improved feed conversion ratio (FCR) for starter phase (1 to 21 d) when compared to the control group. Furthermore, 10% FWB group had significantly increased villus height and villus height/crypt depth ratio in the ileum compared to the control group.Coliformbacteria count in the ileum was lower in the 10% WB group than the control, however, there were no differences between the 10% WB and 10% FWB treatment groups in theC. perfringenscount. There were no morphological changes or incidences of non-specific pathological lesions in the 10% FWB group. These results suggested that replacing 10% of a basal diet with fermented wheat bran could not only improve growth performance but also provide optimal intestinal morphology in broilers. [ABSTRACT FROM PUBLISHER]

Simple Summary: Mushroom waste compost is the main byproduct when cultivating mushrooms. Due to its high mycelium content, mushroom waste compost may improve animal health by increasing antioxidant capacity. Furthermore, increasing evidence suggests that supplementing animal diets with fiber could improve body composition and health. The results showed that supplementation with mushroom waste compost accelerates adipolysis and enhances the antioxidant capacity of broilers. Among all treatment groups, broilers given dietary supplementation with 0.5% mushroom waste compost showed improved feed conversion rate and the highest adipose metabolism. Pennisetum purpureum Schum No. 2 waste mushroom compost (PWMC) is the main byproduct when cultivating Pleurotus eryngii. Due to the high mycelium levels in PWMC, it may have potential as a feed supplement for broilers. This study investigated the effects of PWMC supplementation on antioxidant capacity and adipose metabolism in broilers. In the study, 240 broilers were randomly allocated to one of four treatment groups: basal diet (control), 0.5%, 1%, or 2% PWMC supplementation. Each treatment group had 60 broilers, divided into three replicates. The results showed that supplementation with 0.5% PWMC decreased the feed conversion rate (FCR) from 1.36 to 1.28, compared to the control. Supplementation with 0.5% or 2% PWMC decreased glucose and triglyceride levels, compared to the control (p < 0.0001), the concentrations of adiponectin and oxytocin increased from 5948 to 5709, 11820, and 7938 ng/ mL; and 259 to 447, 873, and 963 pg/ mL, respectively. Toll-like receptor 4 was slightly increased in the 0.5% and 1% PWMC groups. Both interferon-γ (IFN-γ) and interleukin-1ß (IL-1ß) were significantly decreased, by about three to five times for IFN-γ (p < 0.0001) and 1.1 to 1.6 times for IL-1ß (p = 0.0002). All antioxidant-related mRNA, including nuclear factor erythroid 2–related factor 2 (Nrf-2) and superoxidase dismutase-1 (SOD-1), increased significantly following PWMC supplementation. Both claudin-1 and zonula occludens 1 increased, especially in the 2% PWMC group. Excitatory amino acid transporter 3 (EAAT3) significantly increased by about 5, 12, and 11 times in the 0.5%, 1%, and 2% PWMC groups. All adipolysis-related mRNA were induced in the PWMC treatment groups, further enhancing adipolysis. Overall, 0.5% PWMC supplementation was recommended due to its improving FCR, similar antioxidant capacity, and upregulated adipolysis. [ABSTRACT FROM AUTHOR]

Simple Summary: The stress and anti-nutrient effect caused by environmental problems and animal feed is an urgent problem in poultry production. As ancient probiotics, Aspergillus oryzae and Saccharomyces cerevisiae can effectively improve the immunity of animals. Furthermore, the anti-nutrient object, phytate, reduces nutrition absorption. Therefore, S. cerevisiae or A. oryzae with phytase co-fermentation may help solve these problems. Results show that the addition of a fermentation product can effectively reduce the inflammatory response and drop the number of harmful bacteria in the ileum of broilers. Among them, A. oryzae fermentation product has a better effect than S. cerevisiae fermentation product. Saccharomyces cerevisiae and Aspergillus oryzae are both ancient probiotic species traditionally used as microbes for brewing beer and soy sauce, respectively. This study investigated the effect of adding these two probiotics with phytase fermentation products to the broilers diet. Fermented products possess protease and cellulase, and the activities were 777.1 and 189.5 U/g dry matter (DM) on S. cerevisiae fermented products (SCFP) and 190 and 213.4 U/g DM on A. oryzae fermented products (AOFP), respectively. Liposaccharides stimulated PBMCs to produce nitric oxide to 120 μmol. Both SCFP and AOFP reduced lipopolysaccharides stimulated peripheral blood mononuclear cells (PBMCs) nitric oxide release to 40 and 60 μmol, respectively. Nevertheless, in an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, SCFP and AOFP also increased the survival rate of lipopolysaccharides stimulated PBMCs by almost two-fold compared to the negative control. A total of 240 broilers were divided into four groups as Control, SCFP 0.1% (SCFP), SCFP 0.05% + AOFP 0.05% (SAFP), and AOFP 0.1% (AOFP) groups, respectively. Each group had 20 broilers, and three replicate pens. The results showed that the addition of SCFP, SAFP, and AOFP groups did not affect the growth performances, but increased the jejunum value of villus height and villus: crypt ratio on SAFP and AOFP groups compared to the control and SCFP groups. Furthermore, adding SCFP, SAFP, and AOFP significantly reduced the number of Clostridium perfringens in ileum chyme. SCFP, SAFP, and AOFP significantly reduced the amount of interleukin-1β, inducible nitric oxide synthases, interferon-γ, and nuclear factor kappa B mRNA expression in PBMCs, especially in the AOFP group. In summary, all the SCFP, SAFP, and AOFP groups can be suggested as a functional feed additive since they enhanced villus: crypt ratio and decreased inflammation-related mRNA expression, especially for AOFP group in broilers. [ABSTRACT FROM AUTHOR]