Search

Escherichia coli carrying bla CTX–M–1 mediating resistance to extended-spectrum cephalosporins was recently described as a new genotype in Norwegian broiler production. The aim of this study was to characterize these isolates (n = 31) in order to determine whether the emergence of the genotype was caused by clonal expansion or horizontal dissemination of bla CTX–M–1-carrying plasmids. All included isolates were subjected to whole genome sequencing. Plasmid transferability was determined by conjugation, and plasmid replicons in the transconjugants were described using PCR-based replicon typing. Plasmid sizes were determined using S1 nuclease digestion. Plasmids in a subset of strains were reconstructed and compared to plasmids from broiler production in other European countries. The isolates belonged to nine different sequence types (STs), with the largest group being ST57 (n = 12). The vast majority of bla CTX–M–1-carrying plasmids were conjugative. All transconjugants were positive for the IncI1-Iγ replicon, and several also harbored the IncFIB replicon. Highly similar plasmids were present in different E. coli STs. Additionally, high similarity to previously published plasmids was detected. A reconstructed plasmid from an ST57 isolate harbored both IncI1-Iγ and IncFIB replicons and was considered to be co-integrated. The presence of one large plasmid was confirmed by S1 nuclease digestion. Our results show that dissemination of bla CTX–M–1 in Norwegian broiler production is due to both clonal expansion and horizontal transfer of plasmids carrying bla CTX–M–1. The bla CTX–M–1/IncI1-Iγ plasmids grouped into two main lineages, namely clonal complex (CC)-3 and CC-7. The genetic diversity at both strain and plasmid level indicates multiple introductions to Norway. We also show that the bla CTX–M–1 plasmids circulating in Norwegian broiler production are highly similar to plasmids previously described in other countries. [ABSTRACT FROM AUTHOR]

Corrigendum: bla CTX-M-1/IncI1-I Plasmids Circulating in Escherichia coli From Norwegian Broiler Production Are Related, but Distinguishable Keywords: plasmid; IncI1; cephalosporin resistance; Escherichia coli; broiler; bla CTX-M-1 EN plasmid IncI1 cephalosporin resistance Escherichia coli broiler bla CTX-M-1 1 1 1 07/20/21 20210716 NES 210716 In the published article, there was an error in affiliation B 1 b . Plasmid, IncI1, cephalosporin resistance, Escherichia coli, broiler, bla CTX-M-1. [Extracted from the article]

Plasmid, cephalosporin resistance, Escherichia coli, broiler, IncI1, bla CTX-M-1 Keywords: plasmid; IncI1; cephalosporin resistance; Escherichia coli; broiler; bla CTX-M-1 EN plasmid IncI1 cephalosporin resistance Escherichia coli broiler bla CTX-M-1 N.PAG N.PAG 1 04/10/21 20210324 NES 210324 In the original article, the reference for The Bifrost pipeline was incorrectly written as "commitid 0f2ef57". Corrigendum: bla CTX-M-1 /IncI1-I Plasmids Circulating in Escherichia coli From Norwegian Broiler Production Are Related, but Distinguishable. [Extracted from the article]