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Introduction: Infant sleep is affected by many variables. Breastfeeding could also be one of these and the lack of consensus about this relationship motivated us to investigate the association between infant sleep and breastfeeding. Method: Data collecting questionnaire and Brief Infant Sleep Questionnaire were filled by the parents of infants between 6 to 12 months of age in the outpatient clinics of Bezmialem Vakıf University Hospital Pediatrics Department. Results: Ninety two patients participated and were divided into 3 groups. Group 1 represented the infants, who were breastfed only until six months of age and fed with the combination of mother milk and complementary food after that (n=59); group 2 consisted of infants, who always took the combination (n=26). Infants, who took the combination in the first six months and then only complementary food (n=7) made group 3. The total duration of sleep at night didnot differ between the groups significantly (p=0.614), median values being 570, 540, 540 minutes respectively. What the parents made, when their kids awoke at night, was significantly different between group 1 and 3 (p<0.001). 81.4% of the parents in group 1 breastfed their babies and 71.4% of the parents in group 3 fed their babies with feeder. The start of bedtime routines in groups 1 and 2 also differed significantly (p=0.041); 55.9% of infants in group 1 before 10 p.m., and 73.% of infants in group 2 after 10 p.m. Conclusion: Taking only mother milk without complementary food or formula until six months of age is associated with an earlier start to bedtime routine. Also, the parents, who exclusively breastfed in the first six months tended to breastfeed their babies at night-awakenings more than the parents, who always fed their babies with combination. [ABSTRACT FROM AUTHOR]