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Your search keyword '"Jonas B. Raedler"' showing total 2 results
Start Over Author "Jonas B. Raedler" Topic breast cancer
2 results on '"Jonas B. Raedler"'

1. Automated Prognosis Marker Assessment in Breast Cancers Using BLEACH&STAIN Multiplexed Immunohistochemistry

Author

Tim Mandelkow, Elena Bady, Magalie C. J. Lurati, Jonas B. Raedler, Jan H. Müller, Zhihao Huang, Eik Vettorazzi, Maximilian Lennartz, Till S. Clauditz, Patrick Lebok, Lisa Steinhilper, Linn Woelber, Guido Sauter, Enikö Berkes, Simon Bühler, Peter Paluchowski, Uwe Heilenkötter, Volkmar Müller, Barbara Schmalfeldt, Albert von der Assen, Frank Jacobsen, Till Krech, Rainer H. Krech, Ronald Simon, Christian Bernreuther, Stefan Steurer, Eike Burandt, and Niclas C. Blessin

Subjects
breast cancer, prognosis markers, multiplex fluorescence immunohistochemistry, artificial intelligence, Biology (General), QH301-705.5
Abstract

Prognostic markers in routine clinical management of breast cancer are often assessed using RNA-based multi-gene panels that depend on fluctuating tumor purity. Multiplex fluorescence immunohistochemistry (mfIHC) holds the potential for an improved risk assessment. To enable automated prognosis marker detection (i.e., progesterone receptor [PR], estrogen receptor [ER], androgen receptor [AR], GATA3, TROP2, HER2, PD-L1, Ki67, TOP2A), a framework for automated breast cancer identification was developed and validated involving thirteen different artificial intelligence analysis steps and an algorithm for cell distance analysis using 11+1-marker-BLEACH&STAIN-mfIHC staining in 1404 invasive breast cancers of no special type (NST). The framework for automated breast cancer detection discriminated normal glands from malignant glands with an accuracy of 98.4%. This approach identified that five (PR, ER, AR, GATA3, PD-L1) of nine biomarkers were associated with prolonged overall survival (p ≤ 0.0095 each) and two of these (PR, AR) were found to be independent risk factors in multivariate analysis (p ≤ 0.0151 each). The combined assessment of PR-ER-AR-GATA3-PD-L1 as a five-marker prognosis score showed strong prognostic relevance (p < 0.0001) and was an independent risk factor in multivariate analysis (p = 0.0034). Automated breast cancer detection in combination with an artificial intelligence-based analysis of mfIHC enables a rapid and reliable analysis of multiple prognostic parameters. The strict limitation of the analysis to malignant cells excludes the impact of fluctuating tumor purity on assay precision.

Published
2023
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2. Prognostic role of proliferating CD8

Author

Niclas C, Blessin, Wenchao, Li, Tim, Mandelkow, Hannah L, Jansen, Cheng, Yang, Jonas B, Raedler, Ronald, Simon, Franziska, Büscheck, David, Dum, Andreas M, Luebke, Andrea, Hinsch, Katharina, Möller, Anne, Menz, Christian, Bernreuther, Patrick, Lebok, Till, Clauditz, Guido, Sauter, Andreas, Marx, Ria, Uhlig, Waldemar, Wilczak, Sarah, Minner, Till, Krech, Christoph, Fraune, Doris, Höflmayer, Eike, Burandt, and Stefan, Steurer

Subjects
Renal cell cancer, Pancreatic cancer, CD8-Positive T-Lymphocytes, Prognosis, CD8+ cytotoxic Tcells, Immunohistochemistry, Survival Analysis, Colorectal cancer, Ki-67 Antigen, Lymphocytes, Tumor-Infiltrating, Breast cancer, Tumor microenvironment, Ovarian cancer, Neoplasms, Humans, Original Article, Lymphocyte Count, Gastric cancer, Cell Proliferation, Neoplasm Staging, T-Lymphocytes, Cytotoxic
Abstract

Purpose Expansion of CD8+ cytotoxic Tlymphocytes is a prerequisite for anti-cancer immune activity and has gained interest in the era of immune checkpoint therapy. Methods To understand the CD8+ T cell dynamics in the tumor microenvironment, we used multiplex fluorescence immunohistochemistry to quantitate CD8+ proliferation (Ki67 co-expression) in tissue microarrays from 1107 colorectal, 642 renal cell, 1066 breast, 375 ovarian, 451 pancreatic and 347 gastric cancer samples. Results The density and the percentage of proliferating (Ki67+) CD8+ T cells were both highly variable between tumor types as well as between patients with the same tumor type. Elevated density and percentage of proliferating CD8+ cytotoxic T cells were significantly associated with favorable tumor parameters such as low tumor stage, negative nodal stage (p ≤ 0.0041 each), prolonged overall survival (p ≤ 0.0028 each) and an inflamed immune phenotype (p = 0.0025) in colorectal cancer and, in contrast, linked to high tumor stage, advanced ISUP/Fuhrman/Thoenes grading (each p ≤ 0.003), shorter overall survival (p ≤ 0.0330 each) and an immune inflamed phenotype (p = 0.0094) in renal cell cancer. In breast, ovarian, pancreatic and gastric cancer the role of (Ki67+)CD8+ Tcells was not linked to clinicopathological data. Conclusion Our data demonstrate a tumor type dependent prognostic impact of proliferating (Ki67+)CD8+ Tcells and an inverse impact in colorectal and renal cell cancer. Supplementary Information The online version contains supplementary material available at 10.1007/s13402-021-00601-4.

Published
2021

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