Search

Your search keyword '"Gilardi, Maria C."' showing total 2 results
Start Over Author "Gilardi, Maria C." Topic breast
2 results on '"Gilardi, Maria C."'

1. Proton-irradiated breast cells: molecular points of view.

Author

Bravatà V, Cammarata FP, Minafra L, Pisciotta P, Scazzone C, Manti L, Savoca G, Petringa G, Cirrone GAP, Cuttone G, Gilardi MC, Forte GI, and Russo G

Subjects
Cell Line, Tumor, DNA, Complementary radiation effects, Dose-Response Relationship, Radiation, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic radiation effects, Humans, Inflammation, MCF-7 Cells, Oligonucleotide Array Sequence Analysis, Phenotype, Proton Therapy, Radiation Tolerance genetics, Radiotherapy, Breast radiation effects, Breast Neoplasms radiotherapy, Protons
Abstract

Breast cancer (BC) is the most common cancer in women, highly heterogeneous at both the clinical and molecular level. Radiation therapy (RT) represents an efficient modality to treat localized tumor in BC care, although the choice of a unique treatment plan for all BC patients, including RT, may not be the best option. Technological advances in RT are evolving with the use of charged particle beams (i.e. protons) which, due to a more localized delivery of the radiation dose, reduce the dose administered to the heart compared with conventional RT. However, few data regarding proton-induced molecular changes are currently available. The aim of this study was to investigate and describe the production of immunological molecules and gene expression profiles induced by proton irradiation. We performed Luminex assay and cDNA microarray analyses to study the biological processes activated following irradiation with proton beams, both in the non-tumorigenic MCF10A cell line and in two tumorigenic BC cell lines, MCF7 and MDA-MB-231. The immunological signatures were dose dependent in MCF10A and MCF7 cell lines, whereas MDA-MB-231 cells show a strong pro-inflammatory profile regardless of the dose delivered. Clonogenic assay revealed different surviving fractions according to the breast cell lines analyzed. We found the involvement of genes related to cell response to proton irradiation and reported specific cell line- and dose-dependent gene signatures, able to drive cell fate after radiation exposure. Our data could represent a useful tool to better understand the molecular mechanisms elicited by proton irradiation and to predict treatment outcome., (© The Author(s) 2019. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology.)

Published
2019
Full Text
View/download PDF

2. Proton-irradiated breast cells: molecular points of view

Author

Valentina Bravatà 1, Francesco P. Cammarata 1, Luigi Minafra 1, Pietro Pisciotta 1, 2, Concetta Scazzone 3, Lorenzo Manti 4, Gaetano Savoca 1, Giada Petringa 1, Giuseppe A.P. Cirrone 2, Giacomo Cuttone 2, Maria C. Gilardi 1, 5, Giusi I. Forte 1, Giorgio Russo 1, Bravata, V, Cammarata, F, Minafra, L, Pisciotta, P, Scazzone, C, Manti, L, Savoca, G, Petringa, G, Cirrone, G, Cuttone, G, Gilardi, M, Forte, G, Russo, G, Bravata, V., Cammarata, F. P., Minafra, L., Pisciotta, P., Scazzone, C., Manti, L., Savoca, G., Petringa, G., Cirrone, G. A. P., Cuttone, G., Gilardi, M. C., Forte, G. I., Russo, G., Bravatà, Valentina, Cammarata, Francesco P, Minafra, Luigi, Pisciotta, Pietro, Scazzone, Concetta, Manti, Lorenzo, Savoca, Gaetano, Petringa, Giada, Cirrone, Giuseppe A P, Cuttone, Giacomo, Gilardi, Maria C, Forte, Giusi I, and Russo, Giorgio

Subjects
breast cancer, cDNA microarray, gene signature, proton therapy, radiation, Breast, Breast Neoplasms, Cell Line, Tumor, DNA, Complementary, Dose-Response Relationship, Radiation, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Inflammation, MCF-7 Cells, Oligonucleotide Array Sequence Analysis, Phenotype, Proton Therapy, Radiation Tolerance, Radiotherapy, Protons, DNA, Complementary, Health, Toxicology and Mutagenesis, medicine.medical_treatment, Breast Neoplasms, Cell fate determination, Radiation Tolerance, gene signature, 03 medical and health sciences, 0302 clinical medicine, Breast cancer, breast cancer, Cell Line, Tumor, Regular Paper, medicine, proton therapy, Humans, Radiology, Nuclear Medicine and imaging, Breast, Clonogenic assay, Biology, Proton therapy, Oligonucleotide Array Sequence Analysis, 030304 developmental biology, Inflammation, cDNA microarray, 0303 health sciences, Radiotherapy, Chemistry, Gene Expression Profiling, radiation, Cancer, Dose-Response Relationship, Radiation, Gene signature, medicine.disease, Gene Expression Regulation, Neoplastic, Gene expression profiling, Radiation therapy, Phenotype, 030220 oncology & carcinogenesis, MCF-7 Cells, Cancer research, Female, Protons
Abstract

Breast cancer (BC) is the most common cancer in women, highly heterogeneous at both the clinical and molecular level. Radiation therapy (RT) represents an efficient modality to treat localized tumor in BC care, although the choice of a unique treatment plan for all BC patients, including RT, may not be the best option. Technological advances in RT are evolving with the use of charged particle beams (i.e. protons) which, due to a more localized delivery of the radiation dose, reduce the dose administered to the heart compared with conventional RT. However, few data regarding proton-induced molecular changes are currently available. The aim of this study was to investigate and describe the production of immunological molecules and gene expression profiles induced by proton irradiation. We performed Luminex assay and cDNA microarray analyses to study the biological processes activated following irradiation with proton beams, both in the non-tumorigenic MCF10A cell line and in two tumorigenic BC cell lines, MCF7 and MDA-MB-231. The immunological signatures were dose dependent in MCF10A and MCF7 cell lines, whereas MDA-MB-231 cells show a strong pro-inflammatory profile regardless of the dose delivered. Clonogenic assay revealed different surviving fractions according to the breast cell lines analyzed. We found the involvement of genes related to cell response to proton irradiation and reported specific cell line- and dose-dependent gene signatures, able to drive cell fate after radiation exposure. Our data could represent a useful tool to better understand the molecular mechanisms elicited by proton irradiation and to predict treatment outcome

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results