1. Donor Brain Death Leads to a Worse Ischemia-Reperfusion Injury and Biliary Injury After Liver Transplantation in Rats.
- Author
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Chen S, Fang H, Li J, Shi JH, Zhang J, Wen P, Wang Z, Cao S, Yang H, Pan J, Tang H, Zhang H, Guo W, and Zhang S
- Subjects
- Animals, Liver metabolism, Male, Rats, Rats, Inbred Lew, Reperfusion Injury metabolism, Brain Death, Liver pathology, Liver Transplantation methods, Reperfusion Injury pathology, Tissue Donors supply & distribution
- Abstract
Background: Brain-dead (BD) donor is the main source for liver transplantation (LT). We aim to investigate the effect of brain death on donor liver inflammatory activity and its association with ischemia-reperfusion (I/R) injury and biliary tract injury after LT., Material and Method: A brain death model using male Lewis rats was established, in both BD and non-BD groups; livers were harvested for transplantation using a 2-cuff technique. The rats were sacrificed 12 hours (n = 10) or 4 weeks (n = 10) after transplantation. I/R injury and long-term biliary tract injury were observed after transplantation., Results: All rats survived for 4 weeks after transplantation. At 12 hours after BD-donor LT (BDDLT), liver injury worsened; serum transaminases, bilirubin, oxidative stress, inflammatory responses and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining level substantially increased (P < .05). At 4 weeks after BDDLT, serum bilirubin and bile lactate dehydrogenase and γ-glutamyl transpeptidase levels were elevated (P < .05). Biliary fibrosis and epithelial-mesenchymal transition (EMT) were detectable and NDRG1 gene expression was decreased., Conclusions: These results suggested that brain death-induced inflammatory response in donor organs and resulted in a worse I/R injury and biliary tract injury after LT in rats. The brain death-related biliary tract injury may be associated with the regulation of EMT through NDRG1., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2020
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