1. Interferon-stimulated gene 60 (ISG60) constitutes a negative feedback loop in the downstream of TLR3 signaling in hCMEC/D3 cells.
- Author
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Imaizumi, Tadaatsu, Sassa, Naoko, Kawaguchi, Shogo, Matsumiya, Tomoh, Yoshida, Hidemi, Seya, Kazuhiko, Shiratori, Toshihiro, Hirono, Koji, and Tanaka, Hiroshi
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INTERFERONS , *PSYCHOLOGICAL feedback , *CELLULAR signal transduction , *TOLL-like receptors , *ENDOTHELIAL cells - Abstract
Abstract Brain capillary endothelial cells are the component of blood brain barrier, and the first line of defense against viruses invading into brain. We demonstrate that treatment of hCMEC/D3 cells, a human brain capillary endothelial cell line, with a Toll-like receptor 3 (TLR3) agonist polyinosinic-polycytidylic acid (poly IC) induces the expression of interferon (IFN)-stimulated gene 60 (ISG60), and this reaction was mediated by IFN-β. Knockdown of ISG60 increased the poly IC-induced expression of IFN-β and an IFN-β-inducible chemokine CXCL10. This indicates that ISG60 constitutes a negative feedback loop in the downstream of TLR3/IFN-β. ISG60 in brain capillary endothelial cells may contribute to prevent excess immune reactions associated with viral infections. Graphical abstract Unlabelled Image Highlights • TLR3 signaling in human brain endothelial cells • TLR3 signaling induces ISG60. • IFN-beta is involved in ISG60 expression. • ISG60 negatively regulates IFN-beta and CXCL10. • IFN-beta and ISG60 constitute a negative feedback loop. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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