1. Neural transplantation of human MSC and NT2 cells in the twitcher mouse model.
- Author
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Croitoru-Lamoury J, Williams KR, Lamoury FM, Veas LA, Ajami B, Taylor RM, and Brew BJ
- Subjects
- Animals, Astrocytes metabolism, Astrocytes pathology, Brain pathology, Brain Neoplasms metabolism, Brain Neoplasms pathology, Cell Line, Tumor, Disease Models, Animal, Humans, Leukodystrophy, Globoid Cell metabolism, Leukodystrophy, Globoid Cell pathology, Mesoderm pathology, Mice, Mice, Mutant Strains, Multipotent Stem Cells pathology, Neoplasm Transplantation methods, Neoplasms, Experimental metabolism, Neoplasms, Experimental pathology, Neurons metabolism, Neurons pathology, Oligodendroglia metabolism, Oligodendroglia pathology, Transplantation, Heterologous, Brain metabolism, Cell Differentiation, Leukodystrophy, Globoid Cell therapy, Mesoderm metabolism, Multipotent Stem Cells metabolism, Stem Cell Transplantation
- Abstract
Background: Accumulating evidence has demonstrated that the NT2 embryonal carcinoma cell line and multipotential stem cells found in BM, mesenchymal stromal cells (MSC), have the ability to differentiate into a wide variety of cell types. This study was designed to explore the efficacy of these two human stem cell types as a graft source for the treatment of demyelinating disorders such as Krabbe's disease and multiple sclerosis (MS)., Methods: We examined the engraftment and in vivo differentiation of adult MSC and NT2 cells after transplantation into two demyelinating environments, the neonatal and postnatal twitcher mouse brain., Results: Both types of xenografts led to anatomical integration, without tumor formation, and remained viable in the normal and twitcher mouse brain, showing differentiation into neurons, astrocytes and oligodendrocytes., Discussion: This study represents a platform for further stem cell transplantation studies in the twitcher model and potentially has important therapeutic implications.
- Published
- 2006
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