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All three forms of recombinant low voltage-activated T-type Ca(2)(+) channels (Ca(v)3.1, Ca(v)3.2 and Ca(v)3.3) exhibit a small, though clearly evident, window T-type Ca(2)(+) current (I(Twindow)) which is also present in native channels from different neuronal types. In thalamocortical (TC) and nucleus reticularis thalami (NRT) neurones, and possibly in neocortical cells, an I(Twindow)-mediated bistability is the key cellular mechanism underlying the expression of the slow (< 1 Hz) sleep oscillation, one of the fundamental EEG rhythms of non-REM sleep. As the I(Twindow)-mediated bistability may also represent one of the cellular mechanisms underlying the expression of high frequency burst firing in awake conditions, I(Twindow) is of critical importance in neuronal population dynamics associated with different behavioural states.

It has been demonstrated in juvenile rodents that the inhibitory neurons of the nucleus reticularis thalami (NRT) communicate with each other via connexin 36-based electrical synapses. However, whether functional electrical synapses persist into adulthood is not fully known. Here we show that in the presence of the metabotropic glutamate receptor agonists, either trans-ACPD (100 μM) or DHPG (100 μM), 15% of neurons in slices of the adult cat NRT maintained in vitro exhibit stereotypical spikelets with several properties that indicate that they reflect action potentials that have been communicated through an electrical synapse. In particular, these spikelets (1) display a conserved, all-or-nothing waveform with a pronounced after-hyperpolarization (AHP), (2) exhibit an amplitude and time to peak that are unaffected by changes in membrane potential, (3) always occur rhythmically with the precise frequency increasing with depolarization, and (4) are resistant to blockers of conventional, fast, chemical synaptic transmission. Thus, these results indicate that functional electrical synapses in the NRT persist into adulthood where they are likely to serve as an effective synchronizing mechanism for the wide variety of physiological and pathological rhythmic activities displayed by this nucleus. [ABSTRACT FROM PUBLISHER]

All three forms of recombinant low voltage-activated T-type Ca2+ channels (Cav3.1, Cav3.2 and Cav3.3) exhibit a small, though clearly evident, window T-type Ca2+ current (ITwindow) which is also present in native channels from different neuronal types. In thalamocortical (TC) and nucleus reticularis thalami (NRT) neurones, and possibly in neocortical cells, anITwindow-mediated bistability is the key cellular mechanism underlying the expression of the slow (<1 Hz) sleep oscillation, one of the fundamental EEG rhythms of non-REM sleep. As theITwindow-mediated bistability may also represent one of the cellular mechanisms underlying the expression of high frequency burst firing in awake conditions,ITwindow is of critical importance in neuronal population dynamics associated with different behavioural states. [ABSTRACT FROM AUTHOR]