1. Histamine-3 receptor antagonists reduce superoxide anion generation and lipid peroxidation in rat brain homogenates.
- Author
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Badenhorst HE, Maharaj DS, Malan SF, Daya S, and van Dyk S
- Subjects
- Animals, Antioxidants pharmacology, Aspirin pharmacology, Brain metabolism, Disease Models, Animal, Dose-Response Relationship, Drug, Imidazoles pharmacology, In Vitro Techniques, Male, Malondialdehyde antagonists & inhibitors, Piperidines pharmacology, Potassium Cyanide adverse effects, Rats, Rats, Wistar, Receptors, Histamine H3 drug effects, Thiourea analogs & derivatives, Thiourea pharmacology, Brain drug effects, Histamine Agonists pharmacology, Lipid Peroxidation drug effects, Neuroprotective Agents pharmacology, Neurotoxicity Syndromes drug therapy, Superoxides antagonists & inhibitors
- Abstract
Using a cyanide model to induce neurotoxic effects in rat brain homogenates, we examined the neuroprotective properties of three H3 antagonists, namely clobenpropit, thioperamide and impentamine, and compared them to aspirin, a known neuroprotective agent. Superoxide anion levels and malondialdehyde concentration were assessed using the nitroblue tetrazolium and lipid peroxidation assays. Clobenpropit and thioperamide significantly reduced superoxide anion generation and lipid peroxidation. Impentamine reduced lipid peroxidation at all concentrations used, but only reduced superoxide anion generation at a concentration of 1 mM. In the lipid peroxidation assay, all the drugs compared favourably to aspirin. This study demonstrates the potential of these agents to be neuroprotective by exerting antioxidant effects.
- Published
- 2005
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