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Tobacco streak ilarvirus (TSV) seed transmission was investigated by reciprocal pollinations as well as by serological and infectivity assays of flower parts from beans (Phaseolus vulgaris) systematically infected with either TSV pathotype I (isolate Mel 40) or TSV pathotype II (isolate Mel F). Heathly and TSV-infected Black Turtle Soup (BTS) bean plants were reciprocally pollinated by using anthers from either heathly plants or plants systematically infected with Mel 40 or Mel F. When anthers from plants infected with Mel 40 were used to pollinate heathly plants, a high percentage of the resulting seedlings were infected with that isolate (...)

Two pathotypes of tobacco streak virus (TSV), designated I and II, were isolated from naturally infected bean (Phaseolus vulgaris), chickpea (Cicer arietinum), adzuki bean (Vigna angularis), fenugreek (Trigonella foenum-graecum), alfalfa (Medicago sativa), and white sweet clover (Melilotus alba) at Central Ferry, WA. The tow pathotypes could be distinguished by their host reactions, serology, and by SDS-polyacrylamide gel electrophoresis. Pathotype I infected cowpea (Vigna unguiculata), but pathotype II did not (.)

Genetic diversity and incidence of seedborne bean common mosaic virus (BCMV) in Centro Internacional de Agricultura Tropical (CIAT) Phaseolus vulgaris germ plasm seed samples was compared to seed samples of the USDA accessions from which the CIAT samples were originally derived. Following approximately three seed-increase cycles at CIAT during which individual plants obviously infected with BCMV were eliminated, overall and within-sample diversity in 120 seed samples had declined by 45 and 66%, respectively. BCMV incidence also declined by 30%, from an average of 6% in 26 USDA samples to 4.2% in the CIAT samples, but tolerant and visually symptomless infections became more common in the CIAT samples (...)