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Your search keyword '"Wu, Xiang-Long"' showing total 2 results
Start Over Author "Wu, Xiang-Long" Topic bone resorption
2 results on '"Wu, Xiang-Long"'

1. Quercetin prevents bone loss in hindlimb suspension mice via stanniocalcin 1-mediated inhibition of osteoclastogenesis.

Author

Niu YB, Yang YY, Xiao X, Sun Y, Zhou YM, Zhang YH, Dong D, Li CR, Wu XL, Li YH, and Mei QB

Subjects
Animals, Bone Resorption pathology, Bone and Bones drug effects, Bone and Bones pathology, Hindlimb Suspension, Male, Mice, Inbred C57BL, Osteoclasts drug effects, RANK Ligand metabolism, Reactive Oxygen Species metabolism, Bone Density Conservation Agents therapeutic use, Bone Resorption prevention & control, Glycoproteins metabolism, Osteogenesis drug effects, Quercetin therapeutic use
Abstract

Recent studies demonstrate that diet quercetin (Quer) has obvious bone protective effects on ovariectomized rodents but thus far there is no direct evidence to support the inhibitory effect of Quer on bone loss caused by long-term unloading. In the present study, we investigated whether Quer could prevent bone loss induced by unloading in mice. Mice were subjected to hindlimb suspension (HLS) and received Quer (25, 50, 100 mg· kg -1  ·day -1 , ig) for 4 weeks. Before euthanasia blood sample was collected; the femurs were harvested and subjected to MicroCT analysis. We showed that Quer administration markedly improved bone microstructure evidenced by dose-dependently reversing the reduction in bone volume per tissue volume, trabecular number, and bone mineral density, and the increase of trabecular spacing in mice with HLS. Analysis of serum markers and bone histometric parameters confirmed that Quer at both middle and high doses significantly decreased bone resorption-related markers collagen type I and tartrate-resistant acid phosphatase 5b, and increased bone formation-related marker procollagen 1 N-terminal propeptide as compared with HLS group. Treatment with Quer (1, 2, 5 μM) dose-dependently inhibited RANKL-induced osteoclastogenesis through promoting the expression of antioxidant hormone stanniocalcin 1 (STC1) and decreasing ROS generation; knockdown of STC1 blocked the inhibitory effect of Quer on ROS generation. Knockdown of STC1 also significantly promoted osteoclastogenesis in primary osteoclasts. In conclusion, Quer protects bones and prevents unloading-caused bone loss in mice through STC1-mediated inhibition of osteoclastogenesis. The findings suggest that Quer has the potential to prevent and treat off-load bone loss as an alternative supplement.

Published
2020
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2. The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone Resorption In Vitro

Author

Xiang-Long Wu, Yuankun Zhai, Chenrui Li, Qi-Bing Mei, Cory J. Xian, Tingli Lu, Yalei Pan, Yin-Bo Niu, Wutu Fan, Zhai, Yuan-Kun, Pan, Ya-Lei, Niu, Yin-Bo, Li, Chen-Rui, Wu, Xiang-Long, Fan, Wu-Tu, Lu, Ting-Li, Mei, Qi-Bing, and Xian, Cory J

Subjects
musculoskeletal diseases, medicine.medical_specialty, Stromal cell, Article Subject, Endocrinology, Diabetes and Metabolism, lcsh:Diseases of the endocrine glands. Clinical endocrinology, Bone resorption, osthole, Endocrinology, Osteoprotegerin, Internal medicine, medicine, bone marrow stroma cell, Osteopontin, lcsh:RC648-665, biology, Endocrine and Autonomic Systems, business.industry, Acid phosphatase, osteoporosis, Resorption, RANKL, biology.protein, Osteocalcin, business, Research Article
Abstract

Osteoporosis treatment always aimed at keeping the balance of bone formation and bone resorption. Recently, prenyl group in natural products has been proposed as an active group to enhance the osteogenesis process. Osthole has both the prenyl group and bone-protective activities, but the relationship is still unknown. In this study we found that osthole exerted a potent ability to promote proliferation and osteogenic function of rat bone marrow stromal cells and osteoblasts, including improved cell viability, alkaline phosphatase activity, enhanced secretion of collagen-I, bone morphogenetic protein-2, osteocalcin and osteopontin, stimulated mRNA expression of insulin-like growth factor-1, runt-related transcription factor-2, osterix, OPG (osteoprotegerin), RANKL (receptor activator for nuclear factor-κB ligand), and the ratio of OPG/RANKL, as well as increasing the formation of mineralized nodules. However, 7-methoxycoumarin had no obvious effects. Osthole also inhibited osteoclastic bone resorption to a greater extent than 7-methoxycoumarin, as shown by a lower tartrate-resistant acid phosphatase activity and lower number and smaller area of resorption pits. Our findings demonstrate that osthole could be a potential agent to stimulate bone formation and inhibit bone resorption, and the prenyl group plays an important role in these bone-protective effects.

Published
2014

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