1. DNA repair gene expressions are related to bone marrow cellularity in myelodysplastic syndrome.
- Author
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Ribeiro HL Jr, Maia ARS, de Oliveira RTG, Costa MB, Farias IR, de Paula Borges D, de Sousa JC, Magalhães SMM, and Pinheiro RF
- Subjects
- Adult, Aged, Aged, 80 and over, Ataxia Telangiectasia Mutated Proteins genetics, BRCA1 Protein genetics, BRCA2 Protein genetics, Biopsy, Bone Marrow Examination, DNA Breaks, Double-Stranded, DNA Breaks, Single-Stranded, DNA Ligase ATP genetics, DNA-Binding Proteins genetics, Female, Gene Expression Regulation, Enzymologic, Genetic Markers, Humans, Kaplan-Meier Estimate, Male, Middle Aged, Myelodysplastic Syndromes mortality, Predictive Value of Tests, Real-Time Polymerase Chain Reaction, Transcription Factors genetics, Xeroderma Pigmentosum Group A Protein genetics, Young Adult, Bone Marrow Cells pathology, DNA Repair, DNA Repair Enzymes genetics, Myelodysplastic Syndromes genetics, Myelodysplastic Syndromes pathology
- Abstract
Objective: To evaluate the expression of genes related to nuclear excision ( ERCC8 , XPA and XPC ), homologous recombination and non-homologous end-joining ( ATM , BRCA1 , BRCA2 and LIG4 ) repair mechanisms, using quantitative PCR methodologies, and it relation with bone marrow cellularity in myelodysplastic syndrome (MDS)., Methods and Results: A total of 51 adult de novo patients with MDS (3 refractory anaemia (RA), 11 refractory anaemia with ringed sideroblasts (RARS), 28 refractory cytopenia with multilineage dysplasia (RCMD), 3 refractory anaemia with excess blasts type I (RAEB-I), 5 refractory anaemia with excess blasts type II (RAEB-II), and 1 chronic myelomonocytic leukaemia (CMML) were evaluated. For karyotype, 16.2% patients were defined as very low prognosis, 59.5% low risk, 8.1% intermediate risk, 5.4% high risk and 10.8% very high risk. For bone marrow cellularity, 17.6%, 17.6% and 64.7% presented as hypocellular, normocellular and hypercellular, respectively. Patients with hypocellular MDS had significantly decreased expression of ATM (p=0.000) , BRCA1 (p=0.014), BRCA2 (p=0.003) , LIG4 (p=0.004) and ERCC8 (p=0.000) than those with normocellular/hypercellular bone marrow, whereas XPA (p=0.049) and XPC (p=0.000) genes were increased. In patients with hypoplastic MDS, a low expression of ATM (p=0.0268), LIG4 (p=0.0199) and ERCC8 (p=0.0493) was significantly associated with the presence of chromosomal abnormalities. We detected positive correlations between BRCA1 and BRCA2 (r=0.416; p=0.007), ATM and LIG4 (r=0.472; p=0.001), LIG4 and BRCA1 (r=0.333; p=0.026), LIG4 and BRCA2 (r=0.334; p=0.025), ATM and XPA (r=0.377; p=0.008), ATM and XPC (r=0.287; p=0.046), LIG4 and XPC (r=0.371; p=0.007) and XPA and XPC genes (r=0.895; p=0.0000). We also found among all patients evaluated that correlation with LIG4 occurred most often., Conclusions: These correlations demonstrate the important intrinsic relations between single and double DNA strand breaks genes in MDS, emphasising that these genes are related to MDS pathogenesis., Competing Interests: Competing interests: None declared., (Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.)
- Published
- 2017
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