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Your search keyword '"Roman Perik-Zavodskii"' showing total 2 results
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2 results on '"Roman Perik-Zavodskii"'

1. Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing

Author

Roman Perik-Zavodskii, Olga Perik-Zavodskaia, Julia Shevchenko, Vera Denisova, Saleh Alrhmoun, Marina Volynets, Valeriy Tereshchenko, Konstantin Zaitsev, and Sergey Sennikov

Subjects
nucleated erythroid cells, red blood cells, bone marrow, foetal liver, umbilical cord blood, single-cell RNA sequencing, Cytology, QH573-671
Abstract

Nucleated erythroid cells (NECs) are the precursors of erythrocytes. They can be found in various hematopoietic tissues or in the blood. Recently, they have been shown to be active players in immunosuppression through the synthesis of arginase-2 and reactive oxygen species. In this work, we studied NECs in adult bone marrow, umbilical cord blood, and foetal liver parenchyma using single-cell RNA sequencing and found that: (1) all studied NECs expressed the same set of genes, which was enriched in “GO biological process” immunity-related terms; (2) early and late NECs had differential expression of the genes associated with immunosuppression, cell cycle progression, apoptosis, and glycolysis; (3) NECs from different tissues of origin had differential expression of the genes associated with immunosuppression.

Published
2022
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2. Immune Transcriptome and Secretome Differ between Human CD71+ Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma

Author

Roman Perik-Zavodskii, Olga Perik-Zavodskaya, Yulia Shevchenko, Vera Denisova, Kirill Nazarov, Irina Obleuhova, Konstantin Zaitsev, and Sergey Sennikov

Subjects
Adult, Arginase, Erythroid Cells, Liver, Bone Marrow, CD71+ erythroid cells, bone marrow, fetal liver parenchyma, MIF, IL8, TLR1, TLR2, TLR9, NOD2, Genetics, Humans, RNA, Messenger, Transcriptome, Genetics (clinical), Secretome
Abstract

CD71+ erythroid cells (CECs) were only known as erythrocyte progenitors not so long ago. In present times, however, they have been shown to be active players in immune regulation, especially in immunosuppression by the means of ROS, arginase-1 and arginase-2 production. Here, we uncover organ-of-origin differences in cytokine gene expression using NanoString and protein production using Bio-Plex between CECs from healthy human adult bone marrow and from human fetal liver parenchyma. Namely, healthy human adult bone marrow CECs both expressed and produced IFN-a, IL-1b, IL-8, IL-18 and MIF mRNA and protein, while human fetal liver parenchyma CECs expressed and produced IFN-a, IL15, IL18 and TNF-b mRNA and protein. We also detected TLR2 and TLR9 gene expression in both varieties of CECs and TLR1 and NOD2 gene expression in human fetal liver parenchyma CECs only. These observations suggest that there might be undiscovered roles in immune response for CECs.

Published
2022
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