1. Factors influencing urea space estimates in goats
- Author
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Asmare, A., Dawson, L.J., Puchala, R., Gipson, T.A., Villaquiran, M., I.Tovar-Luna, Animut, G., Ngwa, T., Sahlu, T., Merkel, R.C., and Goetsch, A.L.
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RUMINANTS , *ARTIODACTYLA , *MAMMALS , *ZOOLOGY - Abstract
Abstract: Female Alpine goats, 18 approximately 17 months of age (yearling) and 18 approximately 5-month-old (growing), were used in an experiment to determine effects of animal age, urea dose (100, 130, and 160mg/kg BW), and time without feed and water (shrink; 0, 16, and 24h) on urea space (US) estimates. A 20% (w/v) urea solution was infused into a jugular vein, with blood sampled before infusion and every 3min to 21min. BW was 49.8, 47.4, and 47.0kg for yearlings and 26.1, 24.6, and 23.9kg for growing animals after 0, 16, and 24h shrinks, respectively (S.E.=0.80). Time of urea equilibration with body water, determined by a grafted polynomial quadratic–linear model, was affected by a dose×age×shrink interaction (P <0.05); yearling means did not differ (ranging from 7.3 to 10.8min), although those for growing animals were greater (P <0.05) for 0h:130mg (13.0min) and 24h:130mg (13.2min) compared with 24h:100mg (7.6min) and 16h:130mg (7.1min). Based on these times, 12-min samples were used to determine urea space. Urea space was influenced by an age×shrink interaction (P <0.05), being similar among shrink times for yearlings (17.8, 18.8, and 18.9kg) and greater (P <0.05) for growing animals after 0 than 24h shrink (12.9, 11.3, and 10.0kg for 0, 16, and 24h, respectively). Hemoglobin concentration in plasma, as an index of hemolysis, was lower (P <0.05) for growing than for yearling animals (1.16% versus 1.86%), lowest among doses (P <0.05) for 100mg (1.05, 1.74, and 1.75% for 100, 130, and 160mg, respectively), and highest among shrink times (P <0.05) for 24h (1.46, 1.42, and 1.61% for 0, 16, and 24h, respectively). In conclusion, effects of and interactions involving some of the factors studied and high variability in the time of urea equilibration with body water indicate that, regardless of the particular urea space procedures chosen, relatively high numbers of observations are warranted. [Copyright &y& Elsevier]
- Published
- 2007
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