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Protein in breast milk has an influence on the growth and development of babies. Protein itself has a function that cannot be replaced by other nutrients, namely building and maintaining cells and body tissues. Protein is also an antibody, functioning in the body's defense mechanism against various diseases and infections. Giving breast milk to babies can prevent morbidity and mortality in babies and toddlers because breast milk contains many nutrients that are important for the body. The aim of this study was to analyze the effect of BMI on breast milk protein levels in breastfeeding mothers. The design of this research is observational analytic with a cross sectional approach, using logistic regression statistical tests. The results of research on the effect of BMI on breast milk protein levels show that p-value = 0.049 with Exp(B) = 0.004. Based on this research, it can be concluded that there is an influence of BMI on breast milk protein levels. [ABSTRACT FROM AUTHOR]