1. Isolation of lectins by affinity chromatography with porcine plasma proteins immobilized on agarose.
- Author
-
Kajiya A, Koyama Y, Mita T, Mega T, Hase S, Kawakami T, Honda E, Munakata H, and Isemura M
- Subjects
- Amino Acid Sequence, Amino Acids analysis, Amino Acids chemistry, Animals, Aralia chemistry, Blood Proteins metabolism, Electrophoresis, Polyacrylamide Gel methods, Molecular Sequence Data, Plant Lectins chemistry, Plant Lectins metabolism, Swine, Wasabia chemistry, Blood Proteins chemistry, Chromatography, Affinity methods, Plant Lectins isolation & purification, Sepharose chemistry
- Abstract
To develop a convenient method to isolate lectins, we prepared an affinity gel by coupling plasma proteins with agarose beads under conditions where the pH did not exceed 7.5. The validity of the use of this affinity gel in combination with elution using a hapten saccharide was confirmed by isolation of concanavalin A from Jack bean meal. Successful application of the method was demonstrated by isolation of two novel vegetable lectins from udo (Aralia cordate) and wasabi (Wasabia japonica). The method would be useful to isolate new lectins from various sources including plant and animal tissues.
- Published
- 2003
- Full Text
- View/download PDF