1. Force-activatable biosensor enables single platelet force mapping directly by fluorescence imaging.
- Author
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Wang Y, LeVine DN, Gannon M, Zhao Y, Sarkar A, Hoch B, and Wang X
- Subjects
- Animals, Biosensing Techniques methods, Blood Platelets drug effects, Blood Platelets metabolism, Dogs, Drug Evaluation, Preclinical instrumentation, Drug Evaluation, Preclinical methods, Equipment Design, Humans, Integrins metabolism, Optical Imaging methods, Platelet Aggregation Inhibitors pharmacology, Tirofiban, Tyrosine analogs & derivatives, Tyrosine pharmacology, Biosensing Techniques instrumentation, Blood Platelets cytology, Cell Adhesion drug effects, Optical Imaging instrumentation, Platelet Activation drug effects
- Abstract
Integrin-transmitted cellular forces are critical for platelet adhesion, activation, aggregation and contraction during hemostasis and thrombosis. Measuring and mapping single platelet forces are desired in both research and clinical applications. Conventional force-to-strain based cell traction force microscopies have low resolution which is not ideal for cellular force mapping in small platelets. To enable platelet force mapping with submicron resolution, we developed a force-activatable biosensor named integrative tension sensor (ITS) which directly converts molecular tensions to fluorescent signals, therefore enabling cellular force mapping directly by fluorescence imaging. With ITS, we mapped cellular forces in single platelets at 0.4µm resolution. We found that platelet force distribution has strong polarization which is sensitive to treatment with the anti-platelet drug tirofiban, suggesting that the ITS force map can report anti-platelet drug efficacy. The ITS also calibrated integrin molecular tensions in platelets and revealed two distinct tension levels: 12-54 piconewton (nominal values) tensions generated during platelet adhesion and tensions above 54 piconewton generated during platelet contraction. Overall, the ITS is a powerful biosensor for the study of platelet mechanobiology, and holds great potential in antithrombotic drug development and assessing platelet activity in health and disease., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2018
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