1. Sex hormone-binding globulin (SHBG) is a potential early diagnostic biomarker for gastric cancer
- Author
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Che Chang Chang, Yun Ru Liu, Ruei Ting Chang, Hsiu Ming Shih, Ching Yu Lin, Fu An Li, Yudha Patria, and Chao Wen Cheng
- Subjects
Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Apolipoprotein B ,Globulin ,Blotting, Western ,Enzyme-Linked Immunosorbent Assay ,LC‐MS/MS ,03 medical and health sciences ,proteomics ,0302 clinical medicine ,Sex hormone-binding globulin ,Stomach Neoplasms ,Tandem Mass Spectrometry ,Sex Hormone-Binding Globulin ,Internal medicine ,Blood plasma ,Biomarkers, Tumor ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,SHBG ,Biomarker discovery ,Chromatography, High Pressure Liquid ,Early Detection of Cancer ,Original Research ,mass spectrometry ,Aged ,biology ,business.industry ,Clinical Cancer Research ,Cancer ,Middle Aged ,medicine.disease ,Blood proteins ,Healthy Volunteers ,Endocrinology ,Oncology ,Case-Control Studies ,030220 oncology & carcinogenesis ,biology.protein ,Biomarker (medicine) ,Female ,030211 gastroenterology & hepatology ,Gastric cancer ,business ,Biomarkers ,hormones, hormone substitutes, and hormone antagonists - Abstract
The use of blood plasma biomarkers in gastric cancer (GC) management is limited due to a lack of reliable biomarkers. An LC‐MS/MS assay and a bioinformatic analysis were performed to identify blood plasma biomarkers in a GC discovery cohort. The data obtained were verified and validated by western blotting and an ELISA in an independent study cohort. A label‐free quantification analysis of the MS data using PEAKS7 software found that four plasma proteins of apolipoprotein C‐1, gelsolin, sex hormone‐binding globulin (SHBG), and complement component C4‐A were significantly overexpressed in GC patients. A western blot assay of these plasma proteins showed that only SHBG was consistently overexpressed in the patient group. ELISA measurement of SHBG blood plasma levels confirmed that the patient group had significantly higher SHBG levels than the control group. SHBG levels in the patient group remained significantly higher after being stratified by gender, age, and disease stage. These findings show that LC‐MS/MS is powerful and highly sensitive for plasma biomarker discovery, and SHBG could be a potential plasma biomarker for GC management.
- Published
- 2017