1. Gastric mucosal devitalization reduces adiposity and improves lipid and glucose metabolism in obese rats.
- Author
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Oberbach A, Schlichting N, Heinrich M, Kullnick Y, Retschlag U, Lehmann S, Khashab MA, Kalloo AN, and Kumbhari V
- Subjects
- Adiposity, Animals, Bile Acids and Salts metabolism, C-Reactive Protein immunology, Cholesterol metabolism, Cholesterol, HDL metabolism, Cholesterol, LDL metabolism, Diet, High-Fat, Enzyme-Linked Immunosorbent Assay, Fatty Acids, Nonesterified metabolism, Gastric Mucosa pathology, Ghrelin metabolism, Glucagon-Like Peptide 1 metabolism, Glucose, Glucose Tolerance Test, Interleukin-6 immunology, Intra-Abdominal Fat metabolism, Liver metabolism, Male, Obesity immunology, Peptide YY metabolism, Rats, Rats, Sprague-Dawley, Triglycerides metabolism, Argon Plasma Coagulation methods, Blood Glucose metabolism, Gastrectomy methods, Gastric Mucosa surgery, Insulin Resistance, Lipid Metabolism, Obesity metabolism, Stomach surgery
- Abstract
Background and Aims: The gastric mucosa is an endocrine organ that regulates satiation pathways by expression of orexigenic and anorexigenic hormones. Vertical sleeve gastrectomy (VSG) excludes gastric mucosa and reduces gastric volume. Our study aimed to investigate the independent effects of altering gastric mucosa on obesity and its related comorbidities., Methods: Gastric mucosa devitalization (GMD) of 70% of the stomach was achieved by argon plasma coagulation in a high-fat diet rat model and was compared with VSG and sham surgery. In an 8-week follow-up study, we quantified body weight, visceral adiposity, insulin resistance index, cholesterol profiles, and free fatty acid profiles by enzyme-linked immunosorbent assay (ELISA). Following a 2-hour oral glucose tolerance test, the kinetics of ghrelin, glucagon-like peptide-1, peptide YY, and serum and liver bile acid levels were measured. Liver lipid content was quantified by ELISA., Results: GMD resulted in significant reductions in body weight, visceral and subcutaneous adipose tissue, and hepatic steatosis as well as an improvement in lipid metabolism. GMD resulted in significant reductions in food intake and intestinal malabsorption of free fatty acids, both contributing to improved body composition and metabolic profile. Mechanistically, GMD resulted in a significant reduction in serum palmitate levels as well as an increase in serum and liver bile acid levels, known to alter glucose and lipid metabolism. Similar changes were noted when VSG rats were compared with sham surgery rats., Conclusions: Devitalization of gastric mucosa, independent of altering gastric volume, was able to reduce obesity-related comorbidities. The gastric mucosa may be a potential target for treating obesity and its associated comorbidities., (Copyright © 2018 American Society for Gastrointestinal Endoscopy. Published by Elsevier Inc. All rights reserved.)
- Published
- 2018
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