1. Protein C activator from the venom of Agkistrodon blomhoffi ussuriensis retards thrombus formation in the arterio-venous shunt in rats
- Author
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Strukova Sm, Bashkov Gv, Kogan Ae, E.P. Romanova, V.A. Makarov, and I.D. Bobruskin
- Subjects
Male ,Platelet Aggregation ,Molecular Sequence Data ,Biology ,Pharmacology ,Thrombin ,Arteriovenous Shunt, Surgical ,Platelet Adhesiveness ,Fibrinolytic Agents ,Species Specificity ,Platelet adhesiveness ,Crotalid Venoms ,medicine ,Animals ,Platelet ,Amino Acid Sequence ,Thrombus ,Rats, Wistar ,Blood Coagulation ,Chromatography, High Pressure Liquid ,Dose-Response Relationship, Drug ,Activator (genetics) ,Factor V ,Thrombosis ,Hematology ,medicine.disease ,Factor V Inactivation ,Rats ,Enzyme Activation ,Immunology ,Intercellular Signaling Peptides and Proteins ,Partial Thromboplastin Time ,Peptides ,Plasminogen activator ,Protein C ,medicine.drug - Abstract
Protein C (PC) is an anticoagulant protein which, being activated by thrombin, degrades factors V/Va and VIII/VIIIa and releases a tissue-type plasminogen activator. Some Agkistrodon snake venoms contain PC activators which, in experiments, exert an anticoagulant action. An antithrombotic effect of the PC activator from the venom of A. blomhoffi ussuriensis on the model of thrombus formation in the arterio-venous shunt in rats was under investigation. Administration of the PC activator resulted in a dose-dependent prolongation of the thrombus formation time and a decrease in plasma PC activity, which were accompanied by a decrease in factor V activity and APTT prolongation. No reliable changes in the t-PA level, ADP- and epinephrine-induced platelet aggregation were observed. Platelet adhesion to glass beads diminished. We assume that the antithrombotic effect of the PC activator from the A. blomhoffi venom in the platelet-dependent thrombosis model is caused by PC activation and subsequent factor V inactivation as well as by platelet adhesiveness reduction.
- Published
- 1993