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The goal of the research was to determine how the dose of the feed additive Optigen in the ration of cows affects their haematological indices. The cattle were divided into four groups of 10 cows each. During the research, the animals of the control group received their basic ration, while the animals of the experimental groups were additionally fed with the 80, 100, and 120 g of feed additive Optigen. The blood indices were studied through generally accepted methods on certified equipment. It was discovered that 100 g of the feed additive Optigen in the ration of cows during the lactation period significantly improves the cellular components of the blood, increases the content of hemoglobin, total protein, albumins, and globulins, reduces the betaglobulin index, and provides a threshold level of the enzymes Alanine transaminase (ALT) and Aspartate transaminase (AST). The authors determined the blood indices of high-producing cows at the lactation peak characterizing the optimal Optigen dose of 100 g in the ration with 44.95% of dry basis. The obtained data can be used to develop a criterion for controlling the introduction of the feed additive Optigen in the ration of cattle, with the milk production level, feeding ration, and physiological state taken into account. [ABSTRACT FROM AUTHOR]