1. Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts.
- Author
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Bogliotti YS, Wu J, Vilarino M, Okamura D, Soto DA, Zhong C, Sakurai M, Sampaio RV, Suzuki K, Izpisua Belmonte JC, and Ross PJ
- Subjects
- Animals, Biomarkers, Cell Culture Techniques veterinary, Cell Differentiation, Cells, Cultured, Cloning, Organism, Embryo Culture Techniques veterinary, Epigenesis, Genetic, Gene Expression Regulation, Developmental physiology, Nuclear Transfer Techniques veterinary, Blastocyst physiology, Cattle embryology, Embryonic Stem Cells physiology, Pluripotent Stem Cells physiology
- Abstract
Embryonic stem cells (ESCs) are derived from the inner cell mass of preimplantation blastocysts. From agricultural and biomedical perspectives, the derivation of stable ESCs from domestic ungulates is important for genomic testing and selection, genome engineering, and modeling human diseases. Cattle are one of the most important domestic ungulates that are commonly used for food and bioreactors. To date, however, it remains a challenge to produce stable pluripotent bovine ESC lines. Employing a culture system containing fibroblast growth factor 2 and an inhibitor of the canonical Wnt-signaling pathway, we derived pluripotent bovine ESCs (bESCs) with stable morphology, transcriptome, karyotype, population-doubling time, pluripotency marker gene expression, and epigenetic features. Under this condition bESC lines were efficiently derived (100% in optimal conditions), were established quickly (3-4 wk), and were simple to propagate (by trypsin treatment). When used as donors for nuclear transfer, bESCs produced normal blastocyst rates, thereby opening the possibility for genomic selection, genome editing, and production of cattle with high genetic value., Competing Interests: The authors declare no conflict of interest.
- Published
- 2018
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