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ThePseudomonas fluorescensisolate 1 (Pf1) was found to protect the ragi [Eleusine coracana(L.) Gaertner] blast fungus,Pyricularia grisea. Induction of defense proteinsviz.chitinase,ß-1,3 glucanase, peroxidase (PO) and polyphenol oxidase (PPO) by the Pf1 isolate was studied againstP. grisea. Chitinase in a resistant, susceptible and commonly used cultivar with and without challenge inoculation ofP. grisea, revealed changes in the isoform pattern by UV illumination after staining the gel with fluorescent brightner 28. Native PAGE (polyacrylamide gel electrophoresis) of PO showed the single isoform in all the treatments including the control and a significant increase in the intensity of the band in the inoculated control and Pf1 treatment in all the varieties. Isoform analysis of PPO showed the induction of PPO inP. fluorescenstreated plants challenged withP. grisea. [ABSTRACT FROM AUTHOR]