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Objective: Silver sulfadiazine and benzalkonium chloride are biocidal agents known to be effective against microorganisms. Although planktonic cells are easily affected by these agents, bacteria in the biofilm are more resistant to the same chemicals. Agents fixed on nanoparticles are reported to have higher antimicrobial activity against biofilm bacteria. The effects of nanotechnological disinfectants on biofilm bacteria vary with dose and time. The type of surface on which these disinfectants are sprayed also changes their activity. The aim of this study was to investigate the ability of silver sulfadiazine and benzalkonium immobilized on nanoparticles to adhere to surfaces and their effect on biofilm. Methods: Stainless steel coupons were sprayed with nano benzalkonium chloride and nano silver sulfadiazine were air-dried to cure for 24 hours, then the coupons were placed into the biofilm reactor. Bacterial counts in the biofilm layer formed on these surfaces were compared at monthly intervals with control coupons. Bacterial biofilms allowed to form naturally were analyzed by microbiological culture methods and number of live and dead microorganisms were determined by DAPI - CTC staining on epifluorescence microscope. Results: After the test periods, microbial culture and DAPI - CTC (live / dead) results were found significantly lower on disinfectant covered coupons. Furthermore, nano benzalkonium chloride was found to be significantly more effective against biofilm bacteria than nano silver sulfadiazine. Conclusion: The results were examined in order to observe biofilm formation on the surfaces covered with nano benzalkonium chloride and nano silver sulfadiazine compounds, to evaluate their use in industrial and clinical fields as effective disinfectants. Because these disinfectants are frequently used in industrial or clinical environments, results have made an important contribution to the manufacturer to focus on final version of the product. [ABSTRACT FROM AUTHOR]

Rapid developing nanotechnology has provided creation of new products in nano size finding usage in science and technology applications. Nanoparticles provide benefits on different scientific fields as well as using them in production of industrial products. Especially, production of disinfectants using antimicrobial properties of nanoparticles increases against microorganisms that are industrially and clinically important. Silver sulfadiazine and the benzalkonium which are examples of the quaternary ammonium compounds, has been used for a long time as a disinfectant, have been known that they show bacteriostatic and bacteriocidal effect on bacteria, some viruses, fungus and protozoa according to the concentration. Some bacteria such as Staphylococcus aureus, Pseudomonas aeruginosa, Eschericha coli and Klebsiella pneumoniae are clinically and industrially harmful. In addition, these bacteria can cause economic problems with their biofilm structure. Indefensible planktonic cells are affected by this circumstance very easily. The protected biofilm bacteria continue to resist against the disinfectant. The aim of the recent study is to examine minimum bactericidal concentration (MBC) values of nano silver sulfadiazine and nano benzalkonium chloride against planktonic and biofilm forms of these bacteria to determine time and concentration. The minimal effective dose in biofilms were determined as 512 µl ml-1 for nano silver sulfadiazin and 128 µl ml-1 for nano benzalkonium chloride, respectively. Results of this study were evaluated by the producer to determine the final concentration of nano silver sulfadiazin and nano benzalkonium chloride. [ABSTRACT FROM AUTHOR]