1. High-Yield Characterization of Single Molecule Interactions with DeepTip TM Atomic Force Microscopy Probes.
- Author
-
Corregidor D, Tabraue R, Colchero L, Daza R, Elices M, Guinea GV, and Pérez-Rigueiro J
- Subjects
- Microscopy, Atomic Force methods, Streptavidin chemistry, Biotin chemistry, Models, Biological
- Abstract
Single molecule interactions between biotin and streptavidin were characterized with functionalized DeepTip
TM probes and used as a model system to develop a comprehensive methodology for the high-yield identification and analysis of single molecular events. The procedure comprises the covalent binding of the target molecule to a surface and of the sensing molecule to the DeepTipTM probe, so that the interaction between both chemical species can be characterized by obtaining force-displacement curves in an atomic force microscope. It is shown that molecular resolution is consistently attained with a percentage of successful events higher than 90% of the total number of recorded curves, and a very low level of unspecific interactions. The combination of both features is a clear indication of the robustness and versatility of the proposed methodology.- Published
- 2022
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