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Your search keyword '"Leśniowska-Nowak, Justyna"' showing total 2 results
Start Over Author "Leśniowska-Nowak, Justyna" Topic biotechnology
2 results on '"Leśniowska-Nowak, Justyna"'

1. Molecular identification and chromosomal localization of new powdery mildew resistance gene Pm11 in oat

Author

Ociepa, Tomasz, Okoń, Sylwia, Nucia, Aleksandra, Leśniowska-Nowak, Justyna, Paczos-Grzęda, Edyta, and Bisaga, Maciej

Subjects
Genetic Markers, 0106 biological sciences, Avena, Population, molecular identification, Genes, Plant, 01 natural sciences, Chromosomes, Plant, marker-assisted selection, resistance, 03 medical and health sciences, Ascomycota, Avena sterilis, fungal diseases, resistance gene, Chromosome Segregation, Genotype, plant breeding, Genetics, Plant breeding, education, Gene, Crosses, Genetic, Selection (genetic algorithm), Disease Resistance, Plant Diseases, 030304 developmental biology, 0303 health sciences, education.field_of_study, biology, Reproducibility of Results, food and beverages, General Medicine, biology.organism_classification, chromosomal localization, Seedlings, Original Article, powdery mildew, Identification (biology), Agronomy and Crop Science, Powdery mildew, 010606 plant biology & botany, Biotechnology
Abstract

The appropriate selection of various traits in valuable plants is very important for modern plant breeding. Effective resistance to fungal diseases, such as powdery mildew, is an example of such a trait in oats. Marker-assisted selection is an important tool that reduces the time and cost of selection. The aims of the present study were the identification of dominant DArTseq markers associated with a new resistance gene, annotated as Pm11 and derived from Avena sterilis genotype CN113536, and the subsequent conversion of these markers into a PCR-based assay. Among the obtained 30,620 silicoDArT markers, 202 markers were highly associated with resistance in the analysed population. Of these, 71 were selected for potential conversion: 42 specific to resistant and 29 to susceptible individuals. Finally, 40 silicoDArT markers were suitable for primer design. From this pool, five markers, 3 for resistant and 2 for susceptible plants, were selected for product amplification in the expected groups. The developed method, based on 2 selection markers, provides certain identification of resistant and susceptible homozygotes. Also, the use of these markers allowed the determination of heterozygotes in the analysed population. Selected silicoDArT markers were also used for chromosomal localization of new resistance genes. Five out of 71 segregating silicoDArT markers for the Pm11 gene were found on the available consensus genetic map of oat. Five markers were placed on linkage groups corresponding to Mrg12 on the Avena sativa consensus map.

Published
2019

2. Using intervarietal substitution lines for the identification of wheat chromosomes involved in early responses to water-deficit stress.

Author

Dudziak, Karolina, Bulak, Piotr, Zapalska, Magdalena, Börner, Andreas, Szczerba, Hubert, Leśniowska-Nowak, Justyna, and Nowak, Michał

Subjects
PLANT chromosomes, CHROMOSOMES, PLANT plasma membranes, WHEAT, BOTANY, REGULATOR genes, PLANT physiology
Abstract

Water deficit induces reactive oxygen species (ROS) overproduction, which in turn inhibits plant growth and development. High concentrations of ROS disrupt the osmotic balance in plant cells and alter membrane integrity. Chromosomes carrying structural or regulatory genes must be detected to better understand plant response mechanisms to stress. The aim of our study was to identify Triticum aestivum L. chromosomes involved in early responses to short-term water-deficit stress (1, 3 and 6 h). In the present study, intervarietal substitution lines of drought-tolerant 'Saratovskaya 29' and sensitive 'Janetzkis Probat' wheat cultivars were examined. We studied the biochemical plant response system and conducted an analysis of catalase, ascorbate peroxidase and guaiacol peroxidase activities, levels of lipid peroxidation and changes in relative water content. Our results determined that the first reaction was a significant increase in guaiacol peroxidase (GPX) activity. However, the strongest impact on plant responses was found for catalase (CAT), which caused a significant decrease in lipid peroxidation (LPO) levels. Our findings indicate that chromosomes 5A, 4B, 6B and 7D are associated with early responses to short-term osmotic stress in wheat. [ABSTRACT FROM AUTHOR]

Published
2019
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