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Abstract Microbial pathogens have threatened the world due to their pathogenicity and ability to spread in communities. The conventional laboratory‐based diagnostics of microbes such as bacteria and viruses need bulky expensive experimental instruments and skilled personnel which limits their usage in resource‐limited settings. The biosensors‐based point‐of‐care (POC) diagnostics have shown huge potential to detect microbial pathogens in a faster, cost‐effective, and user‐friendly manner. The use of various transducers such as electrochemical and optical along with microfluidic integrated biosensors further enhances the sensitivity and selectivity of detection. Additionally, microfluidic‐based biosensors offer the advantages of multiplexed detection of analyte and the ability to deal with nanoliters volume of fluid in an integrated portable platform. In the present review, we discussed the design and fabrication of POCT devices for the detection of microbial pathogens which include bacteria, viruses, fungi, and parasites. The electrochemical techniques and current advances in this field in terms of integrated electrochemical platforms that include mainly microfluidic‐ based approaches and smartphone and Internet‐of‐things (IoT) and Internet‐of‐Medical‐Things (IoMT) integrated systems have been highlighted. Further, the availability of commercial biosensors for the detection of microbial pathogens will be briefed. In the end, the challenges while fabrication of POC biosensors and expected future advances in the field of biosensing have been discussed. The integrated biosensor‐based platforms with the IoT/IoMT usually collect the data to track the community spread of infectious diseases which would be beneficial in terms of better preparedness for current and futuristic pandemics and is expected to prevent social and economic losses.

A fluorescence probe comprising boronic acid-functionalized tungsten disulfide quantum dots for the label-free detection of dopamine was developed. The detection method is based on the selective interaction between diols and aminophenylboronic acid. This interaction generates quenching of fluorescence due to aggregation and the inner filter effect of the fabricated fluorescence probe. The proposed method allows the determination of dopamine in the concentration range from 0.05 μM to 100 μM including a correlation coefficient of 0.9925 along with a low detection limit of 0.01 μM. It manifests a better selectivity towards dopamine in the existence of different interfering analytes. Furthermore, the applicability of the proposed method has been utilized in the real sample which showed promising results for diagnostic purposes.