Search

In this study the multiple metal(loid) (As, Cd, Cu and Ni) resistant bacterium Serratia sp. KUJM3 was able to grow in both single and multiple metal(loid) contaminated wastewater and removed them by 34.93–48.80% and 22.93–32%, respectively. It reduced As(v) to As(III) by 68.44–85.06% in a concentration dependent manner. The strain's IAA production potential increased significantly under both metal(loid)s regime. The lentil (Lens culinaris) seed germination and seed production were enhanced with the exogenous bacterial inoculation by 20.39 and 16.43%, respectively. Under both multi-metal(loid) regimes the bacterial inoculation promoted shoot length (22.65–51.34%), shoot dry weight (33.89–66.11%) and seed production (13.46–35%). Under bacterial manipulation the metal(loid)s immobilization increased with concomitant curtailment of translocation in lentil plant by 61.89–75.14% and 59.19–71.14% in shoot and seed, respectively. The strain biomineralized struvite (MgNH 4 PO 4 ·6H 2 O) from human urine @ 403 ± 6.24 mg L−1. The fertilizer potential of struvite was confirmed with the promotion of cowpea (Vigna unguiculata) growth traits e.g. leaf number (37.04%), pod number (234%), plant wet weight (65.47%) and seed number (134.52%). Thus Serratia sp. KUJM3 offers multiple benefits of metal(loid)s bioremediation, As(V) reduction, plant growth promotion, and struvite biomineralization garnering a suite of appealing environmental applications. [ABSTRACT FROM AUTHOR]

The microbial infections due to biofilm forming bacterial pathogens are very common in human subjects. The intensive application of antibiotics in integrated disease management strategy has led to increased multidrug resistance incommon pathogens. Thus, indicating need of developing an alternative method for the control of these multidrug resistant pathogens. Present study involves the Moringa oleifera aqueous extract mediated biological synthesis of silver (Ag nanoparticles (NPs)- Avg. size 82.5 nm; zeta potential = −27.9 mV), copper oxide (CuONPs- Avg. size 61 nm; zeta potential = −19.3 mV), iron oxide (FeONPs- Avg. size 83.3 nm; zeta potential = −9.37 mV) and alumina (AlONPs- Avg. size 87.3 nm; zeta potential = −10.9 mV) nanoparticles. Biological nanoparticles were detected by visual observation, spectrophotometric detection followed by zeta potential analysis, nanoparticle tracking analysis, Fourier transform infrared spectrometry and X-ray diffraction analysis. Nanoparticles were further evaluated for their in vitro antimicrobial potential, membrane damage effectiveness, biofilm inhibition activity by MTT assay. Nanoparticles were assessed against human pathogens viz. two Gram-positive (Bacillus subtilis MTCC 441 and Staphylococcus haemolyticus MTCC 3383) and two Gram-negative bacteria (Enterobacter aerogenes MTCC 111 and Salmonella enterica ser. Typhi MTCC 8767). The nanoparticles exhibited akin activity pattern against all pathogens studied i.e. AgNPs > CuONPs > AlONPs > FeONPs. Tested nanoparticles registered lower MIC values and more intensified growth inhibition against Gram-negative bacteria compared to their Gram-positive counterparts. These results pointed out that the M. oleifera mediated nanoparticles can be prospectivelyutilized in the development of alternative antimicrobials against diverse bacterial infections. [Display omitted] • Ecofriendly M. oleifera mediated metal nanoparticle (NP) synthesis method reported. • Antimicrobial potential of nanoparticles were assessed by inhibition assays. • Bactericidal activity was monitored by Time kill assay and biofilm inhibition assay. • Antibacterial activity pattern of NPs was as: AgNPs > CuONPs > AlONPs > FeONPs. [ABSTRACT FROM AUTHOR]

The microbial infections due to biofilm forming bacterial pathogens are very common in human subjects. The intensive application of antibiotics in integrated disease management strategy has led to increased multidrug resistance incommon pathogens. Thus, indicating need of developing an alternative method for the control of these multidrug resistant pathogens. Present study involves the Moringa oleifera aqueous extract mediated biological synthesis of silver (Ag nanoparticles (NPs)- Avg. size 82.5 nm; zeta potential = −27.9 mV), copper oxide (CuONPs- Avg. size 61 nm; zeta potential = −19.3 mV), iron oxide (FeONPs- Avg. size 83.3 nm; zeta potential = −9.37 mV) and alumina (AlONPs- Avg. size 87.3 nm; zeta potential = −10.9 mV) nanoparticles. Biological nanoparticles were detected by visual observation, spectrophotometric detection followed by zeta potential analysis, nanoparticle tracking analysis, Fourier transform infrared spectrometry and X-ray diffraction analysis. Nanoparticles were further evaluated for their in vitro antimicrobial potential, membrane damage effectiveness, biofilm inhibition activity by MTT assay. Nanoparticles were assessed against human pathogens viz. two Gram-positive (Bacillus subtilis MTCC 441 and Staphylococcus haemolyticus MTCC 3383) and two Gram-negative bacteria (Enterobacter aerogenes MTCC 111 and Salmonella enterica ser. Typhi MTCC 8767). The nanoparticles exhibited akin activity pattern against all pathogens studied i.e. AgNPs > CuONPs > AlONPs > FeONPs. Tested nanoparticles registered lower MIC values and more intensified growth inhibition against Gram-negative bacteria compared to their Gram-positive counterparts. These results pointed out that the M. oleifera mediated nanoparticles can be prospectivelyutilized in the development of alternative antimicrobials against diverse bacterial infections. [Display omitted] • Ecofriendly M. oleifera mediated metal nanoparticle (NP) synthesis method reported. • Antimicrobial potential of nanoparticles were assessed by inhibition assays. • Bactericidal activity was monitored by Time kill assay and biofilm inhibition assay. • Antibacterial activity pattern of NPs was as: AgNPs > CuONPs > AlONPs > FeONPs. [ABSTRACT FROM AUTHOR]