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The productivity of sunflower oil in Indonesia is very limited in terms of quality, quantity, and continuity. This causes Indonesia to have to import sunflower oil from other countries to meet domestic needs. Tocopherol is one of the antioxidant compounds contained in sunflower seeds. Tocopherol production is regulated by some candidate genes, including the hydroxyphenylpyruvate dioxygenase (HPPD) gene. One solution that can be done is to assemble hybrid varieties that have the advantage of being able to produce higher oil through genetic engineering efforts. This study aims to isolate and identify the active domain region of the HPPD gene of sunflower accession HA 15 which is involved in regulating tocopherol biosynthesis to support the assembly of hybrid varieties in sunflower accession HA 15. This study succeeded in isolating and identifying the active domain of the sequence. HPPD gene of sunflower accession HA 15 with a product size of 1570 bp, with a similarity level of 98% with the reference gene with accession number XM_022150035.2. [ABSTRACT FROM AUTHOR]