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Abstract: The work presents a new and highly sensitive disposable amperometric triglyceride (TG) biosensor based on Au/PEDOT-PSS nanocomposite inkjet-printed on screen-printed carbon electrodes (SPCEs) and co-immobilization of lipase, glycerol kinase and glycerol-3-phosphate oxidase. One-step chemically synthesized Au/PEDOT-PSS nanocomposite deposited by inkjet printing is demonstrated to produce in nanorod structures on SPCE surface. The electrochemical responses are found to be significantly enhanced by Au nanoparticles and optimal preparation conditions of gold nanoparticle formation for electrochemical detection are found to be 0.015M EDOT and 6.25mM gold precursor. For TGs determination in blood, SPCE electrode with 5 layers of inkjet printed Au/PEDOT-PSS nanocomposite provides optimum performances when operated with the operating potential of 0.4V in 0.1M sodium phosphate buffer pH of 7.0. Wide dynamic range (0–531mg/dL), moderate sensitivity (2.66μA/mM), modest response time (30s), low detection limit (7.88mg/dL), low interference, good reproducibility and satisfactory life time (40% loss of response current after 30 days of storage at 4°C) have been achieved. Therefore, inkjet-printed Au/PEDOT-PSS nanocomposite on SPCEs is a promising candidate for TGs determination. [Copyright &y& Elsevier]

Highlights: [•] Enyzmatic glucose biosensor is made from GOD/PEDOT-PSS/SPCE. [•] Sensor is formed by one-step electrolytic exfoliation and enzyme linking with glutraldehyde. [•] Quasi-reversible direct electrochemistry of GOD at GP/PEDOT:PSS/SPCE is observed. [•] Graphene inclusion gives 13 times sensitivity increase in GOD/PEDOT:PSS/SPCE. [•] High sensitivity (7.23μA/mM) and low detection limit (0.3μM) are achieved. [ABSTRACT FROM AUTHOR]

Graphene is a highly promising material for biosensors due to its excellent physical and chemical properties which facilitate electron transfer between the active locales of enzymes or other biomaterials and a transducer surface. Printing technology has recently emerged as a low-cost and practical method for fabrication of flexible and disposable electronics devices. The combination of these technologies is promising for the production and commercialization of low cost sensors. In this review, recent developments in organo-functionalized graphene and printed biosensor technologies are comprehensively covered. Firstly, various methods for printing graphene-based fluids on different substrates are discussed. Secondly, different graphene-based ink materials and preparation methods are described. Lastly, biosensing performances of printed or printable graphene-based electrochemical and field effect transistor sensors for some important analytes are elaborated. The reported printed graphene based sensors exhibit promising properties with good reliability suitable for commercial applications. Among most reports, only a few printed graphene-based biosensors including screen-printed oxidase-functionalized graphene biosensor have been demonstrated. The technology is still at early stage but rapidly growing and will earn great attention in the near future due to increasing demand of low-cost and disposable biosensors. [ABSTRACT FROM AUTHOR]