1. Sensing and intracellular imaging of Zn2+ based on affinity peptide using an aggregation induced emission fluorescence “switch-on” probe.
- Author
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He, Xingxing, Wang, Xiaoming, Zhang, Lin, Fang, Guozhen, Liu, Jifeng, and Wang, Shuo
- Subjects
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BIOSENSORS , *BIOLUMINESCENCE , *FLUORIMETRY , *PEPTIDES , *FLUOROPHORES - Abstract
Direct monitoring of Zn 2+ in living system is extremely important to human health. In Zn 2+ sensing, most chemical molecules with aggregation induced emission (AIE) effect were only used in vitro because of its potential biological toxicity. In this work, a biocompatible “turn-on” fluorescent probe for the determination of Zn 2+ combined AIE fluorophore tetraphenylethene (TPE) molecule with peptide has been designed and synthesized based on Schiff base reaction. The TPE-Peptide probe exhibited strong emission in the presence of Zn 2+ due to the formation of self-assembling complexes between Zn 2+ and the three histidine residues in two strands of peptide, which triggered TPE moieties in an aggregated state. The TPE-Peptide probe displays a highly selective and sensitive fluorescence intensity with a relatively wide linear range from 0 to 100 μM and a detection limit of 18.56 nM. As designed, the probe can penetrate live HeLa cells and image intracellular Zn 2+ by turn-on response with low cytotoxicity and good stability, implying that TPE-Peptide acts as a highly useful fluorescent biosensor for biological systems. Our strategy opens a new way for the investigation of other metal ions or bioactive molecules that can bind with special peptides and it may find wide applications in biological sensing. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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