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Resumen del trabajo presentado en la 3rd Coatings and Interfaces Conference, celebrada en online del 24 al 26 de noviembre de 2021, TiO2 thin films and nanostructures functionalized with specific biomolecules have been used in the manufacture of highly sensitive and selective electrochemical biosensors.1 In particular, nanomaterials functionalized with folic acid for detection of folate receptors are highly interesting, because these receptors are over-expressed in most cancer cells and, therefore, their identification can allow the detection and even the photothermal treatment of cancer. 2-3 In this work, a methodology for the manufacture of TiO2 thin films and their functionalization with folic acid is presented. TiO2 thin films were obtained by pulsed laser deposition at different fluence values of the laser light between 1.3 and 2.5 J/cm2 at a constant deposition time of 5 min. The deposition chamber had a base pressure of 7.4x10- 3 Pa and the deposition of the film was carried out on a Si substrate at room temperature, under a pressure of 4.6 Pa of oxygen. Thin films with and without functionalization were characterized by Xray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and Raman spectroscopy. AFM images of the obtained films show that as the fluence of laser light increases, the thickness and RMS roughness of thin film increases. The AFM analysis of the functionalized films evidenced a decrease in roughness, with respect to the non-functionalized film, indicating that the folic acid molecules were mainly anchored in the valleys of the film. The Raman analysis as by XPS showed evidence of the interaction of folic acid with the surface of TiO2 through the pteridine ring. In this way, the reported methodology allows for obtaining TiO2 thin films functionalized with folic acid that can be tested in future research in the detection of cancer cells.