25 results on '"Moscone D"'
Search Results
2. Ultrafiltrate sampling device for continuous monitoring
- Author
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Moscone, D., Venema, K., and Korf, J.
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- 1996
- Full Text
- View/download PDF
3. Sensing the Lactic Acid in Probiotic Yogurts Using an L-Lactate Biosensor Coupled with a Microdialysis Fiber Inserted in a Flow Analysis System.
- Author
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Radoi, A., Moscone, D., and Palleschi, G.
- Subjects
BIOSENSORS ,DEHYDROGENASES ,ELECTRODES ,YOGURT ,LACTATE dehydrogenase - Abstract
An amperometric biosensor for the determination of L-lactic acid in probiotic yogurts has been assembled using L-lactate dehydrogenase (EC 1.1.1.27, LDH) entrapped in 1% (v/v) neutralized Nafion® solution deposited on Variamine blue redox mediator modified screen-printed electrodes. The Variamine blue was previously covalently linked to oxidized single-walled carbon nanotubes and used for modifying screen-printed electrodes. The electrochemical cell, containing the L-lactate biosensor operating at an applied working potential of +200 mV vs. Ag|AgCl, was coupled with a microdialysis fiber and connected with a flow system, thus obtaining a microdialysis based sampling experimental set-up. Various analytical parameters, such as the cofactor concentration (2 mM, NAD+), the flow rate (10.5 μL/min), the applied working potential (+200 mV vs. Ag|AgCl), the working buffer (50 mM phosphate buffer +0.1 M KCl), and pH (7.5), were optimized in batch amperometric experiments. The dynamic linear working range was comprised between 2·10-4 and 1·10-3 M. The proposed biosensor was challenged with real samples of yogurt, properly diluted in working buffer, and the performances of the L-lactate biosensor were compared with a commercially available kit for the determination of L-lactic acid in foodstuffs from R-Biopharm GmbH, Germany, showing a good agreement. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
4. AMPEROMETRIC DETECTION OF BIOGENIC AMINES IN CHEESE USING IMMOBILISED DIAMINE OXIDASE.
- Author
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Compagnone, D., Isoldi, G., Moscone, D., and Palleschi, G.
- Subjects
BIOGENIC amines ,BIOSENSORS ,CHEESE ,CONDUCTOMETRIC analysis - Abstract
Diamine oxidase from Lens culinaris has been used for the amperometric detection of biogenic amines in cheese. The enzyme has been immobilised onto polymeric membranes or glass beads and coupled with a Pt based hydrogen peroxide electrode. The resulting enzyme electrode and reactor have been optimised for the response to the six biogenic amines more frequently found in cheese: histamine, tyramine, putrescine, cadaverine, phenylethylamine and tryptamine. Detection limits were found to be in the micromolar range with a linearity up to 3 orders of magnitude. Rejection of the electrochemically active compounds present in cheese was effective by the electropolymerization of 1,4-diaminebenzene onto the Pt surface. The best performances in terms of sensitivity and selectivity were obtained using the enzyme reactor. [ABSTRACT FROM AUTHOR]
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- 2001
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5. Recent advances in biosensors based on enzyme inhibition.
- Author
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Amine, A., Arduini, F., Moscone, D., and Palleschi, G.
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BIOSENSORS , *ENZYME inhibitors , *ENVIRONMENTAL health , *DRUG analysis , *MEDICAL screening - Abstract
Enzyme inhibitors like drugs and pollutants are closely correlated to human and environmental health, thus their monitoring is of paramount importance in analytical chemistry. Enzymatic biosensors represent cost-effective, miniaturized and easy to use devices; particularly biosensors based on enzyme inhibition are useful analytical tools for fast screening and monitoring of inhibitors. The present review will highlight the research carried out in the last 9 years (2006–2014) on biosensors based on enzyme inhibition. We underpin the recent advances focused on the investigation in new theoretical approachs and in the evaluation of biosensor performances for reversible and irreversible inhibitors. The use of nanomaterials and microfluidic systems as well as the applications of the various biosensors in real samples is critically reviewed, demonstrating that such biosensors allow the development of useful devices for a fast and reliable alarm system. [ABSTRACT FROM AUTHOR]
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- 2016
- Full Text
- View/download PDF
6. Preparation of paper-based devices for reagentless electrochemical (bio)sensor strips
- Author
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Fabiana Arduini, Stefano Cinti, Danila Moscone, Cinti, S., Moscone, D., and Arduini, F.
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Paper ,Computer science ,Nanotechnology ,Biosensing Techniques ,STRIPS ,Reference electrode ,General Biochemistry, Genetics and Molecular Biology ,Phosphates ,law.invention ,03 medical and health sciences ,Software portability ,Silver chloride ,chemistry.chemical_compound ,Settore CHIM/01 ,0302 clinical medicine ,law ,Electrodes ,030304 developmental biology ,0303 health sciences ,Electrochemical Techniques ,Equipment Design ,chemistry ,Filter (video) ,Electrode ,Screen printing ,Biosensor ,030217 neurology & neurosurgery - Abstract
Despite substantial advances in sensing technologies, the development, preparation, and use of self-testing devices is still confined to specialist laboratories and users. Decentralized analytical devices will enormously impact daily lives, enabling people to analyze diverse clinical, environmental, and food samples, evaluate them and make predictions to improve quality of life, particularly in remote, resource-scarce areas. In recent years, paper-based analytical tools have attracted a great deal of attention; the well-known properties of paper, such as abundance, affordability, lightness, and biodegradability, combined with features of printed electrochemical sensors, have enabled the development of sustainable devices that drive (bio)sensors beyond the state of the art. Their blindness toward colored/turbid matrices (i.e., blood, soil), their portability, and the capacity of paper to autonomously filter/purge/react with target species make such devices powerful in establishing point-of-need tools for use by non-specialists. This protocol describes the preparation of a voltammetric phosphate sensor and an amperometric nerve agent biosensor; both platforms produce quantitative measurements with currents in the range of microamperes. These printed strips comprise three electrodes (graphite for working and counter electrodes and silver/silver chloride (Ag/AgCl) for the reference electrode) and nanomodifiers (carbon black and Prussian blue) to improve their performance and specificity. Depending on analytical need, different types of paper (filter, office) and configurations (1D, 2D, 3D) can be adopted. The protocol, based on the use of cost-effective manufacturing techniques such as drop casting (to chemically modify the substrate surface) and wax/screen printing (for creating the channels and electrodes), can be completed in
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- 2019
7. Paper-based synthesis of Prussian Blue Nanoparticles for the development of whole blood glucose electrochemical biosensor
- Author
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Danila Moscone, Fabiana Arduini, Roberto Cusenza, Stefano Cinti, Cinti, S., Cusenza, R., Moscone, D., and Arduini, F.
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Blood Glucose ,Paper ,Surface Properties ,Reducing agent ,Paper-based ,Electrode ,Surface Propertie ,Nanoparticle ,Nanotechnology ,Biosensing Techniques ,02 engineering and technology ,01 natural sciences ,Analytical Chemistry ,Biosensing Technique ,chemistry.chemical_compound ,Settore CHIM/01 - Chimica Analitica ,Glucose oxidase ,Particle Size ,Hydrogen peroxide ,Electrodes ,Prussian blue ,Electrochemical Technique ,biology ,Filter paper ,010401 analytical chemistry ,Electrochemical Techniques ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,BiosensorPaper-basedPoint-of-carePrussian Blue NanoparticlesScreen-printed electrodesWhole blood ,Environmentally friendly ,0104 chemical sciences ,Whole blood ,Prussian Blue Nanoparticle ,chemistry ,Point-of-care ,Screen-printed electrode ,biology.protein ,Nanoparticles ,0210 nano-technology ,Biosensor ,Ferrocyanide ,Ferrocyanides - Abstract
Nowadays, environmentally friendly synthesis pathways for preserving the environment and minimizing waste are strongly required. Herein, we propose filter paper as a convenient scaffold for chemical reactions. To demonstrate this novel approach, Prussian Blue Nanoparticles (PBNPs) were synthesized on filter paper by utilizing few μL of its precursors without external inputs, i.e. pH, voltage, reducing agents, and without producing waste as well. The functional paper, named “Paper Blue”, is successfully applied in the sensing field, exploiting the reduction of hydrogen peroxide at low applied potential. The eco-designed “Paper Blue” was combined with wax- and screen-printing to manufacture a reagentless electrochemical point-of-care device for diabetes self-monitoring, by using glucose oxidase as the biological recognition element. Blood glucose was linearly detected for a wide concentration range up to 25 mM (450 mg/dL), demonstrating its suitability for management of diabetes and glucose-related diseases. The Paper Blue-based biosensor demonstrated a correlation coefficient of 0.987 with commercial glucose strips (Bayer Contour XT). The achieved results demonstrated the effectiveness of this approach, which is also extendible to other (bio)systems to be applied in catalysis, remediation, and diagnostics.
- Published
- 2018
8. Novel carbon black-cobalt phthalocyanine nanocomposite as sensing platform to detect organophosphorus pollutants at screen-printed electrode.
- Author
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Cinti, S., Neagu, D., Carbone, M., Cacciotti, I., Moscone, D., and Arduini, F.
- Subjects
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CARBON-black , *POLLUTANT identification , *PHTHALOCYANINES , *NANOCOMPOSITE materials , *ORGANOPHOSPHORUS compounds , *ELECTRODES - Abstract
A facile “one-step” route to produce a homogenous and highly stable cobalt phthalocyanine (CoPc)-based dispersion by using carbon black (CB) as supporting material is reported. Herein, CB is proposed as effective material to load CoPc in order to obtain a CB/CoPc hybrid nanocomposite dispersion suitable for modifying screen-printed electrodes (SPEs) by an easy and automatable drop casting approach. CoPc resulted anchored to CB by a non-covalent physisorption, confirmed by IR and UV-visible spectroscopies, allowing to preserve the electrochemical performances of CoPc. The resulting CB/CoPc-modified SPE was tested as sensing tool to detect thiocholine, an enzymatic product of butyrylcholinesterase (BChE). The use of CB/CoPc leads to a highly sensitive thiocholine detection by applying a low potential (+0.05 V vs. internal reference) without fouling problem, a typical drawback that affects the thiol electrochemical detection. The favorable characteristics of the sensor were exploited for an easy BChE biosensor fabrication that renders this biosensor well suitable for mass-production. This electrochemical monoenzymatic biosensor was then challenged towards paraoxon, chosen as model organophosphorous pesticide, obtaining a low detection limit (18 nM). The suitability of the biosensor was tested in a waste water sample obtaining satisfactory recovery values, thus demonstrating its capability in such complex matrix. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
9. Carbon black as an outstanding and affordable nanomaterial for electrochemical (bio)sensor design
- Author
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Vincenzo Mazzaracchio, Stefano Cinti, Danila Moscone, Aziz Amine, Viviana Scognamiglio, Fabiana Arduini, Arduini, F., Cinti, S., Mazzaracchio, V., Scognamiglio, V., Amine, A., and Moscone, D.
- Subjects
Paper ,Immunosensors ,DNA sensors ,Materials science ,Nanostructure ,Electrode ,Microfluidics ,Biomedical Engineering ,Biophysics ,DNA sensor ,Nanotechnology ,Enzymatic biosensors ,Biosensing Techniques ,02 engineering and technology ,Immunosensor ,01 natural sciences ,Paper-based biosensor ,Nanomaterials ,Carbon-based nanomaterial ,Biosensing Technique ,Settore CHIM/01 ,Soot ,Carbon based nanomaterials ,Electrochemistry ,Animals ,Humans ,Paper-based biosensors ,Electrodes ,Electrochemical Technique ,Animal ,010401 analytical chemistry ,Electrochemical Techniques ,General Medicine ,Carbon black ,Equipment Design ,021001 nanoscience & nanotechnology ,Nanostructures ,0104 chemical sciences ,Carbon-based nanomaterials ,Enzymatic biosensor ,Bio sensor ,0210 nano-technology ,Biosensor ,Biotechnology ,Human - Abstract
Advances in cutting-edge technologies including nanotechnology, micmfluidics, electronic engineering, and material science have boosted a new era in the design of robust and sensitive biosensors. In recent years, carbon black has been re-discovered in the design of electrochemical (bio)sensors thanks to its interesting electroanalytical properties, absence of treatment requirement, cost-effectiveness (c.a. 1 (sic)/Kg), and easiness in the preparation of stable dispersions. Herein, we present an overview of the literature on carbon black-based electrochemical (bio)sensors, highlighting current trends and possible challenges to this rapidly developing area, with a special focus on the fabrication of carbon black-based electrodes in the realisation of sensors and biosensors (e.g. enzymatic, immunosensors, and DNA-based).
- Published
- 2020
10. Paper-based electrochemical peptide nucleic acid (PNA) biosensor for detection of miRNA-492: a pancreatic ductal adenocarcinoma biomarker
- Author
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Danila Moscone, Stefano Cinti, Concetta Avitabile, Maria Moccia, Veronica Caratelli, Fabiana Arduini, Anna Lisa Imbriani, Biagio Pede, Michele Saviano, Moccia, M., Caratelli, V., Cinti, S., Pede, B., Avitabile, C., Saviano, M., Imbriani, A. L., Moscone, D., and Arduini, F.
- Subjects
Peptide Nucleic Acids ,Serum ,Pancreatic ductal adenocarcinoma ,Biomedical Engineering ,Biophysics ,02 engineering and technology ,Biosensing Techniques ,Adenocarcinoma ,01 natural sciences ,chemistry.chemical_compound ,Settore CHIM/01 ,microRNA ,Ruthenium (III) hexamine ,Electrochemistry ,medicine ,Humans ,Screen-printing ,Peptide nucleic acid ,010401 analytical chemistry ,General Medicine ,Electrochemical Techniques ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Electrochemical gas sensor ,MicroRNAs ,Wax-printing ,medicine.anatomical_structure ,chemistry ,Duplex (building) ,Cancer research ,Differential pulse voltammetry ,0210 nano-technology ,Pancreas ,Biosensor ,Biomarkers ,Biotechnology - Abstract
Pancreatic ductal adenocarcinoma is the predominant neoplastic disease of the pancreas and it represents the fourth most frequent cause of death in cancer-related disease, with only 8% of survivors after 5-year to the diagnosis. The main issues of this type of cancer rely on fast progress (i.e. 14 months from T1 to a T4 stage), nonspecific symptoms with delay in diagnosis, and the absence of effective screening strategies. To address the lack of early diagnosis, we report a cost-effective paper-based biosensor for the detection of miRNA-492, which is recognised as a biomarker for pancreatic ductal adenocarcinoma. To design a miniaturised, sensitive, and robust paper-based platform, an electrochemical sensor was screen-printed on office paper previously wax-patterned via wax-printing technique. The paper-based sensor was then engineered with a novel and highly specific peptide nucleic acid (PNA) as the recognition element. The formation of PNA/miRNA-492 adduct was evaluated by monitoring the interaction between the positively charged ruthenium (III) hexamine with uncharged PNA and/or negatively charged PNA/miRNA-492 duplex by differential pulse voltammetry. The paper-based biosensor provided a linear range up to 100 nM, with a LOD of 6 nM. Excellent selectivity towards one- and two-base mismatches (1MM, 2MM) or scrambled (SCR) sequences was highlighted and the applicability for biomedical analyses was demonstrated, measuring miRNA-492 in undiluted serum samples.
- Published
- 2020
11. Experimental Comparison in Sensing Breast Cancer Mutations by Signal ON and Signal OFF Paper-Based Electroanalytical Strips
- Author
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Emily P. Nguyen, Fabiana Arduini, Claudio Parolo, Giulia Cinotti, Danila Moscone, Stefano Cinti, Arben Merkoçi, Veronica Caratelli, Cinti, S., Cinotti, G., Parolo, C., Nguyen, E. P., Caratelli, V., Moscone, D., Arduini, F., and Merkoci, A.
- Subjects
Paper ,DNA, Single-Stranded ,Breast Neoplasms ,STRIPS ,Biosensing Techniques ,010402 general chemistry ,computer.software_genre ,01 natural sciences ,Signal ,Field (computer science) ,Analytical Chemistry ,law.invention ,Biosensing Technique ,DNA-based biosensors ,Breast cancer ,Settore CHIM/01 ,Design and Development ,law ,Experimental comparison ,Detection methods ,medicine ,Humans ,Liquid biopsy ,Protocol (science) ,Electrochemical Technique ,Chemistry ,010401 analytical chemistry ,Analytical performance ,Electrochemical Techniques ,medicine.disease ,Signal on ,0104 chemical sciences ,Emerging technologies ,Mutation ,Single strand DNA ,Female ,Data mining ,Detection protocols ,Biosensor ,computer ,Breast Neoplasm ,Human - Abstract
Altres ajuts: the ICN2 is funded by the CERCA Programme/Generalitat de Catalunya. The development of paper-based electroanalytical strips as powerful diagnostic tools has gained a lot of attention within the sensor community. In particular, the detection of nucleic acids in complex matrices represents a trending topic, especially when focused toward the development of emerging technologies, such as liquid biopsy. DNA-based biosensors have been largely applied in this direction, and currently, there are two main approaches based on target/probe hybridization reported in the literature, namely Signal ON and Signal OFF. In this technical note, the two approaches are evaluated in combination with paper-based electrodes, using a single strand DNA relative to H1047R (A3140G) missense mutation in exon 20 in breast cancer as the model target. A detailed comparison among the analytical performances, detection protocol, and cost associated with the two systems is provided, highlighting the advantages and drawbacks depending on the application. The present work is aimed to a wide audience, particularly for those in the field of point-of-care, and it is intended to provide the know-how to manage with the design and development stages, and to optimize the platform for the sensing of nucleic acids using a paper-based detection method.
- Published
- 2019
12. A challenge in biosensors: Is it better to measure a photon or an electron for ultrasensitive detection?
- Author
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Patrizia Simoni, Martina Zangheri, Elisa Marchegiani, Laura Fabiani, Aldo Roda, Noemi Colozza, Danila Moscone, Mara Mirasoli, Fabiana Arduini, Roda A., Arduini F., Mirasoli M., Zangheri M., Fabiani L., Colozza N., Marchegiani E., Simoni P., and Moscone D.
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Chemiluminescence ,Biomedical Engineering ,Biophysics ,Amperometry ,Reproducibility of Result ,Nanotechnology ,Electrons ,02 engineering and technology ,Biosensing Techniques ,Immunosensor ,Electron ,01 natural sciences ,Horseradish peroxidase ,Sensitivity and Specificity ,law.invention ,Biosensing Technique ,Settore CHIM/01 ,law ,Electrochemistry ,Electrochemical biosensor ,Enzyme-based biosensor ,Photons ,Electrochemical Technique ,biology ,Chemistry ,Paper-based assay ,010401 analytical chemistry ,Reproducibility of Results ,General Medicine ,Electrochemical Techniques ,021001 nanoscience & nanotechnology ,Photon ,0104 chemical sciences ,Luminescent Measurement ,Luminescent Measurements ,biology.protein ,0210 nano-technology ,Biosensor ,Biotechnology - Abstract
Biosensor development exploiting various transduction principles is characterized by a strong competition to reach high detectability, portability and robustness. Nevertheless, a literature-based comparison is not possible, as different conditions are employed in each paper. Herein, we aim at evaluating which measurement, photons or electrons, yields better biosensor performance. Upon outlining an update in recent achievements to boost analytical performance, amperometry and chemiluminescence (CL)-based biosensors are directly compared employing the same biospecific reagents and analytical formats. Horseradish peroxidase (HRP) and hydrogen peroxide concentrations were directly measured, while glucose and mouse IgG were detected employing an enzyme paper-based biosensor and an immunosensor, respectively. Detectability was down to picomoles of hydrogen peroxide (4 pmol for CL and 210 pmol for amperometry) and zeptomoles of HRP (45 zmol for CL and 20 zmol for amperometry); IgG was detected down to 12 fM (CL) and 120 fM (amperometry), while glucose down to 17 μM (CL) and 40 μM (amperometry). Results showed that amperometric and CL biosensors offered similar detectability and analytical performance, with some peculiarities that suggest complementary application fields. As they generally provided slightly higher detectability and wider dynamic ranges, CL-based biosensors appear more suitable for point-of-care testing of clinical biomarkers, where detectability is crucial. Nevertheless, as high detectability in CL biosensors usually requires longer acquisition times, their rapidity will allocate electrochemical biosensors in real-time monitoring and wearable biosensors. The analytical challenge demonstrated that these biosensors have competitive and similar performance, and between photons and electrons the competition is still open.
- Published
- 2019
13. Origami multiple paper-based electrochemical biosensors for pesticide detection
- Author
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Luca Amendola, Stefano Cinti, Danila Moscone, Fabiana Arduini, Veronica Caratelli, Giuseppe Palleschi, Arduini, F., Cinti, S., Caratelli, V., Amendola, L., Palleschi, G., and Moscone, D.
- Subjects
Paper ,Insecticides ,Biomedical Engineering ,Biophysics ,02 engineering and technology ,Biosensing Techniques ,Standard solution ,Butyrylcholinesterase Alkaline phosphatase Tyrosinase Paraoxon 2,4-dichlorophenoxyacetic acid Atrazine ,01 natural sciences ,Paraoxon ,chemistry.chemical_compound ,Organophosphorus Compounds ,Rivers ,Limit of Detection ,Alkaline phosphatase ,Electrochemistry ,medicine ,Humans ,Settore CHIM/01 - Chimica Analitica ,Atrazine ,Pesticides ,Insecticide ,River ,Prussian blue ,Chromatography ,2,4-dichlorophenoxyacetic acid ,Filter paper ,010401 analytical chemistry ,Water ,General Medicine ,Pesticide ,021001 nanoscience & nanotechnology ,Potentiostat ,0104 chemical sciences ,chemistry ,Butyrylcholinesterase ,Tyrosinase ,Organophosphorus Compound ,0210 nano-technology ,Biosensor ,Butyrylcholinesterase Alkaline phosphatase Tyrosinase Paraoxon 2 ,4-dichlorophenoxyacetic acid Atrazine ,Water Pollutants, Chemical ,Biotechnology ,medicine.drug ,Human - Abstract
Herein, we propose the first three-dimensional origami paper-based device for the detection of several classes of pesticides by combining different enzyme-inhibition biosensors. This device was developed by integrating two different office paper-based screen-printed electrodes and multiple filter paper-based pads to load enzymes and enzymatic substrates. The versatile analysis of different pesticides was carried by folding and unfolding the filter paper-based structure, without any addition of reagents and any sample treatment (i.e. dilution, filtration, pH adjustment). The paper-based platform was employed to detect paraoxon, 2,4-dichlorophenoxyacetic acid, and atrazine by exploiting the capability of these different types of pesticides (i.e. organophosphorus insecticides, phenoxy-acid herbicides, and triazine herbicide) to inhibit butyrylcholinesterase, alkaline phosphatase, and tyrosinase, respectively. The degree of inhibition correlating to the quantity of pesticides was evaluated by chronoamperometrically monitoring the enzymatic activity in the absence and in the presence of pesticides by using a portable potentiostat. To improve the sensitivity, the paper-based electrodes were modified with carbon black alone in the case of platforms for 2,4-dichlorophenoxyacetic acid and atrazine detection, or decorated with Prussian blue nanoparticles for the detection of paraoxon. The paper-based device was applied for the detection of paraoxon, 2,4-dichlorophenoxyacetic acid, and atrazine at ppb level in both standard solutions and river water sample. The accuracy of this origami multiple paper-based electrochemical biosensor was evaluated in river water by recovery studies, obtaining satisfactory values (e.g. for paraoxon 90 ± 1% and 88 ± 2%, for 10 and 20 ppb, respectively). The proposed three-dimensional origami paper device allows for rapid, cost-effective and accurate pesticide detection in surface water as a result of combining filter and office papers, screen-printing, wax-printing and nanomaterial technology.
- Published
- 2019
14. Novel bio-lab-on-a-tip for electrochemical glucose sensing in commercial beverages
- Author
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Danila Moscone, Fabiana Arduini, Vincenzo Mazzaracchio, Stefano Cinti, Roberta Marrone, Cinti, S., Marrone, R., Mazzaracchio, V., Moscone, D., and Arduini, F.
- Subjects
Materials science ,Biomedical Engineering ,Biophysics ,Lab-on-a-tip ,Nanotechnology ,Biosensing Techniques ,02 engineering and technology ,01 natural sciences ,Beverages ,Matrix (chemical analysis) ,Glucose Oxidase ,Electroanalysi ,Settore CHIM/01 ,Electrochemistry ,Miniaturization ,Glucose oxidase ,Beverage ,Electrodes ,Detection limit ,biology ,010401 analytical chemistry ,Pipette ,Electrochemical Techniques ,General Medicine ,Chronoamperometry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Glucose ,Filter (video) ,biology.protein ,0210 nano-technology ,Biosensor ,Biotechnology - Abstract
The development of portable and user-friendly sensing platforms is a hot topic in the field of analytical chemistry. Among others, electroanalytical approaches exhibit a high amenability for reaching this purpose, i.e. the commercial strips for diabetes care are an obvious success. However, providing fully-integrated and reagent-free methods is always a leitmotiv. In this work, we evaluated the use of a disposable pipette tip, opportunely configured to demonstrate the first example of an electrochemical biosystem in a pipette tip, namely bio-lab-on-a-tip. The combination of a pipette tip, wire electrodes, enzyme, and cotton wool filter, allows the fabrication of a novel electroanalytical platform that does not need expertise-required tasks. To demonstrate the feasibility of this novel method, glucose is detected in beverages by means of chronoamperometry. The experimental setup, entirely built inside the pipette tip, is able to 1) block impurities/interferences from matrix, 2) load/release reagents for the bio-assay, 3) reduce the operating task to zero, and 4) perform electrochemical detection. With optimized experimental parameters, the bio-lab-on-a-tip is able to detect glucose linearly up to 10 mM with a detection limit of 170 μM. The effectiveness of the platform was confirmed by testing commercial beverages, e.g. Coca-Cola and Coca-Cola Zero, with high accuracy. In addition, the shelf-life of the novel device was evaluated, highlighting the role of cotton wool filter for providing a suitable environment for glucose oxidase stability. The novel concept can be easily generalized for further applications in the field of non-invasive clinical diagnostics and in-situ environmental monitoring.
- Published
- 2020
15. Acetylcholinesterase biosensor based on single-walled carbon nanotubes—Co phtalocyanine for organophosphorus pesticides detection
- Author
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Ivanov, A.N., Younusov, R.R., Evtugyn, G.A., Arduini, F., Moscone, D., and Palleschi, G.
- Subjects
- *
ACETYLCHOLINESTERASE , *BIOSENSORS , *CARBON nanotubes , *ORGANOPHOSPHORUS compounds , *PESTICIDES , *DETECTORS , *CARBON electrodes , *OXIDATION - Abstract
Abstract: A simple and reliable technique has been developed for the construction of an amperometric acetylcholinesterase biosensor based on screen-printed carbon electrodes. For the first time, one-step modification using single-walled carbon nanotubes and Co phtalocyanine has been proposed to decrease the working potential and to increase the signal of thiocholine oxidation. The biosensor developed made it possible to detect 5–50ppb of paraoxon and 2–50ppb of malaoxon with detection limits of 3 and 2ppb, respectively (incubation 15min). The biosensor showed high reproducibility when measurements of the substrate and inhibitor were performed (R.S.D. about 1% and 2.5%, respectively). The reliability of the inhibition measurements was confirmed by testing spiked samples of sparkling and tape waters. [Copyright &y& Elsevier]
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- 2011
- Full Text
- View/download PDF
16. Novel carbon black-cobalt phthalocyanine nanocomposite as sensing platform to detect organophosphorus pollutants at screen-printed electrode
- Author
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Daniela Neagu, Danila Moscone, Stefano Cinti, Fabiana Arduini, Ilaria Cacciotti, Marilena Carbone, Cinti, S, Neagu, D, Carbone, M, Cacciotti, I, Moscone, D, and Arduini, F
- Subjects
Settore CHIM/03 - Chimica Generale e Inorganica ,Detection limit ,Nanocomposite ,Materials science ,General Chemical Engineering ,Biosensor Hybrid Nanocomposite Carbon Black Cobalt Phthalocyanine Organophosphorus pesticide ,010401 analytical chemistry ,Nanotechnology ,02 engineering and technology ,Carbon black ,021001 nanoscience & nanotechnology ,Electrochemistry ,01 natural sciences ,0104 chemical sciences ,Thiocholine ,Electrode ,Settore CHIM/01 - Chimica Analitica ,0210 nano-technology ,Dispersion (chemistry) ,Biosensor - Abstract
A facile one-step route to produce a homogenous and highly stable cobalt phthalocyanine (CoPc)-based dispersion by using carbon black (CB) as supporting material is reported. Herein, CB is proposed as effective material to load CoPc in order to obtain a CB/CoPc hybrid nanocomposite dispersion suitable for modifying screen-printed electrodes (SPEs) by an easy and automatable drop casting approach. CoPc resulted anchored to CB by a non-covalent physisorption, confirmed by IR and UV-visible spectroscopies, allowing to preserve the electrochemical performances of CoPc. The resulting CB/CoPc-modified SPE was tested as sensing tool to detect thiocholine, an enzymatic product of butyrylcholinesterase (BChE). The use of CB/CoPc leads to a highly sensitive thiocholine detection by applying a low potential (+0.05 V vs. internal reference) without fouling problem, a typical drawback that affects the thiol electrochemical detection. The favorable characteristics of the sensor were exploited for an easy BChE biosensor fabrication that renders this biosensor well suitable for mass-production. This electrochemical monoenzymatic biosensor was then challenged towards paraoxon, chosen as model organophosphorous pesticide, obtaining a low detection limit (18 nM). The suitability of the biosensor was tested in a waste water sample obtaining satisfactory recovery values, thus demonstrating its capability in such complex matrix.
- Published
- 2016
17. How to extend range linearity in enzyme inhibition-based biosensing assays
- Author
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Giuseppe Palleschi, Danila Moscone, Aziz Amine, Stefano Cinti, Fabiana Arduini, Amine, A., Cinti, S., Arduini, F., Moscone, D., and Palleschi, G.
- Subjects
02 engineering and technology ,Biosensing Techniques ,Mass spectrometry ,01 natural sciences ,Analytical Chemistry ,chemistry.chemical_compound ,Biosensing Technique ,Extended linearity ,Bioassay ,Enzyme Inhibitor ,Settore CHIM/01 - Chimica Analitica ,Cholinesterase Inhibitor ,Enzyme Inhibitors ,Chromatography ,Chemistry ,010401 analytical chemistry ,Linearity ,Substrate (chemistry) ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Enzyme inhibition ,Kinetics ,Linear range ,Progress curve ,Reagent ,Costs and Cost Analysi ,enzyme inhibition, progress curve, extended linearity, substrate conversion ,Acetylcholinesterase ,Costs and Cost Analysis ,Substrate conversion ,Cholinesterase Inhibitors ,0210 nano-technology ,Biosensor ,Fluoride - Abstract
Bioassays based on enzyme inhibition are analytical tools widely employed for inhibitor analysis. Beside the conventional analytical techniques such as chromatography and mass spectrometry, these bioassays are cost-effective, easy to use, and suitable for in situ measurement but they are often characterised by a quite narrow linear range. Herein, we report a novel graphical method based on integrated Michaelis-Menten equation, valid for all types of reversible inhibition, which provides an extended linear range. The suitability of this innovative approach was demonstrated in the case of fluoride quantification using a colorimetric bioassay based on acetylcholinesterase inhibition. The “half time reaction”, estimated by the progress curve of cholinesterase inhibition, was plotted versus the fluoride inhibitor concentration, observing an extended linear range up to 5 mM, instead of 0.6 mM using initial rate measurements. The applicability of this new concept was further demonstrated in the case of catalase enzyme inhibited by cyanide. Furthermore, it was demonstrated that fixed substrate conversion at level of 10–50% allows determination of inhibitor concentration in a wide linear range with high precision and in short time of analysis. This novel theoretical and practical approach allows for the extension of the linear range without any further experiments, with several advantages including low reagent consumption, reduced waste generation and time of measurement.
- Published
- 2018
18. Efforts, Challenges, and Future Perspectives of Graphene-Based (Bio)sensors for Biomedical Applications
- Author
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Viviana Scognamiglio, Fabiana Arduini, Danila Moscone, Stefano Cinti, Cinti, S, Scognamiglio, V, Moscone, D, and Arduini, F
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Computer science ,Graphene ,End user ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,World health ,Field (computer science) ,0104 chemical sciences ,law.invention ,Settore CHIM/01 ,law ,Electronics ,0210 nano-technology ,Biosensor - Abstract
Graphene has been emphasized by researchers as the most promising nanomaterial for many application fields, including energy, catalysis, electronics, remediation, and sensing. In the field of analytic sciences, the continuous demand of sensitive, portable, user-friendly, low-cost, and low-volume analyses has highlighted graphene and graphene-related nanocomposites as key components toward the implementation of graphene-based (bio)sensors. This chapter provides the opportunity to summarize and critically evaluate the recent developments of graphene-based electrochemical devices that have been applied in biomedical field, for the detection of a wide variety of analytes of interest ranging from small compounds to nucleic acids, antibodies, proteins, and bacteria. Particular focus will interest graphene-based lab-on-a-chip (LOC) devices that have been (or will be) capable to answer the important query introduced by the World Health Organization (WHO) toward the development of affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free and deliverable to end users (ASSURED) devices and to replace traditional approaches in the biomedical self-diagnostic field.
- Published
- 2018
19. How cutting-edge technologies impact the design of electrochemical (bio)sensors for environmental analysis. A review
- Author
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Fabiana Arduini1, 2, Stefano Cinti1, Viviana Scognamiglio3, Danila Moscone1, Giuseppe Palleschi1, Arduini, F, Cinti, S, Scognamiglio, V, and Moscone, D
- Subjects
Pollutants ,Environmental analysis ,Microfluidics ,Biomimetic design ,Nanotechnology ,Biosensing Techniques ,02 engineering and technology ,Artificial bioreceptors ,01 natural sciences ,Biochemistry ,Printed (bio)sensors ,Analytical Chemistry ,law.invention ,law ,Nanomaterials ,Lab-on-a-chip ,Environmental monitoring ,Environmental Chemistry ,Settore CHIM/01 - Chimica Analitica ,Spectroscopy ,Chemistry ,010401 analytical chemistry ,021001 nanoscience & nanotechnology ,Nanostructures ,0104 chemical sciences ,Enhanced Data Rates for GSM Evolution ,0210 nano-technology ,Biosensor ,Sensing system ,Environmental Monitoring - Abstract
Through the years, scientists have developed cutting-edge technologies to make (bio)sensors more convenient for environmental analytical purposes. Technological advancements in the fields of material science, rational design, microfluidics, and sensor printing, have radically shaped biosensor technology, which is even more evident in the continuous development of sensing systems for the monitoring of hazardous chemicals. These efforts will be crucial in solving some of the problems constraining biosensors to reach real environmental applications, such as continuous analyses in field by means of multi-analyte portable devices. This review (with 203 refs.) covers the progress between 2010 and 2015 in the field of technologies enabling biosensor applications in environmental analysis, including i) printing technology, ii) nanomaterial technology, iii) nanomotors, iv) biomimetic design, and (v) microfluidics. Next section describes futuristic cutting-edge technologies that are gaining momentum in recent years, which furnish highly innovative aspects to biosensing devices.
- Published
- 2017
20. A paper-based nanomodified electrochemical biosensor for ethanol detection in beers
- Author
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Stefano Cinti, Danila Moscone, Fabiana Arduini, Mattia Basso, Cinti, S, Basso, M, Moscone, D, and Arduini, F
- Subjects
Paper ,Paper-based screen-printed electrode ,Nanotechnology ,02 engineering and technology ,Biosensing Techniques ,Electrocatalyst ,01 natural sciences ,Biochemistry ,Pichia ,Analytical Chemistry ,chemistry.chemical_compound ,Carbon black ,Limit of Detection ,Prussian blue nanoparticles ,Electrochemistry ,Environmental Chemistry ,Settore CHIM/01 - Chimica Analitica ,Disposable Equipment ,Electrodes ,Spectroscopy ,Detection limit ,Prussian blue ,Nanocomposite ,Prussian blue nanoparticle ,Ethanol ,Chemistry ,Ethanol oxidase ,Wax printing ,010401 analytical chemistry ,Beer ,021001 nanoscience & nanotechnology ,Enzymes, Immobilized ,0104 chemical sciences ,Dielectric spectroscopy ,Alcohol Oxidoreductases ,Cyclic voltammetry ,0210 nano-technology ,Biosensor ,Nuclear chemistry - Abstract
Herein, we report the first example of a paper-based screen-printed biosensor for the detection of ethanol in beer samples. Common office paper was adopted to fabricate the analytical device. The properties of this paper-based screen-printed electrode (SPE) were investigated by cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy, and they were compared with the well-established polyester-based SPEs as well. Paper demonstrated similar properties when compared with polyester, highlighting suitability towards its utilization in sensor development, with the advantages of low cost and simple disposal by incineration. A nanocomposite formed by Carbon Black (CB) and Prussian Blue nanoparticles (PBNPs), namely CB/PBNPs, was utilized as an electrocatalyst to detect the hydrogen peroxide generated by the enzymatic reaction between alcohol oxidase (AOx) and ethanol. After optimizing the analytical parameters, such as pH, enzyme, concentration, and working potential, the developed biosensor allowed a facile quantification of ethanol up to 10 mM (0.058 %vol), with a sensitivity of 9.13 μA/mM cm2 (1574 μA/%vol cm2) and a detection limit equal to 0.52 mM (0.003%vol). These satisfactory performances rendered the realized paper-based biosensor reliable over the analysis of ethanol contained in four different types of beers, including Pilsner, Weiss, Lager, and alcohol-free. The proposed manufacturing approach offers an affordable and sustainable tool for food quality control and for the realization of different electrochemical sensors and biosensors as well.
- Published
- 2016
21. Nanomaterials in electrochemical biosensors for pesticide detection: advances and challenges in food analysis
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Danila Moscone, Viviana Scognamiglio, Stefano Cinti, Fabiana Arduini, Arduini, F, Cinti, S, Scognamiglio, V, and Moscone, D
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Materials science ,Aptamer ,Nanotechnology ,02 engineering and technology ,Carbon nanotube ,01 natural sciences ,Analytical Chemistry ,Nanomaterials ,law.invention ,law ,enzymatic biosensor ,Electrochemical biosensor ,Settore CHIM/01 - Chimica Analitica ,nanomaterials ,immunosensor ,carbon nanotubes ,Graphene ,food analysis ,010401 analytical chemistry ,Aptasensor ,021001 nanoscience & nanotechnology ,biosensors ,Food Analysis ,0104 chemical sciences ,nanorods ,nanoparticles ,0210 nano-technology ,Biosensor - Abstract
This overview (with 114 refs.) covers the progress made between 2010 and 2015 in the field of nanomaterial based electrochemical biosensors for pesticides in food. Its main focus is on strategies to analyze real samples. The review first gives a short introduction into the most often used biorecognition elements. These include (a) enzymes (resulting in inhibition-based and direct catalytic biosensors), (b) antibodies (resulting in immunosensors), and (c) aptamers (resulting in aptasensors). The next main section covers the various kinds of nanomaterials for use in biosensors and includes carbonaceous species (carbon nanotubes, graphene, carbon black and others), and non-carbonaceous species in the form of nanoparticles, rods, or porous materials. Aspects of sample treatment and real sample analysis are treated next before discussing vanguard technologies in tailor-made food analysis. [Figure not available: see fulltext.]
- Published
- 2016
22. Cholesterol biosensor based on inkjet-printed Prussian blue nanoparticle-modified screen-printed electrodes
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Fabiana Arduini, Danila Moscone, Laura Gonzalez-Macia, Giuseppe Palleschi, Stefano Cinti, Anthony J. Killard, Cinti, S, Arduini, F, Moscone, D, Palleschi, G, Gonzalez-Macia, L, and Killard, A J
- Subjects
Inkjet-printing ,Materials science ,Cholesterol oxidase ,Nanoparticle ,Nanotechnology ,Prussian blue nanoparticles ,Screen-printed electrodes ,Encapsulation ,Biosensor ,Cholesterol ,chemistry.chemical_compound ,Materials Chemistry ,Settore CHIM/01 - Chimica Analitica ,Electrical and Electronic Engineering ,Instrumentation ,Inkjet printing ,Reproducibility ,Prussian blue ,Metals and Alloys ,Condensed Matter Physics ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Low volume ,chemistry ,Electrode - Abstract
Here we describe the construction and optimization of a cholesterol biosensor based on screen-printed electrodes (SPEs) modified with inkjet-printed Prussian blue nanoparticles (PBNPs). The deposition of PBNPs using inkjet printing led to the highly facile fabrication of sensors with excellent sensitivity and reproducibility for the measurement of H 2 O 2 . Further integration of the sensor with a microfabricated low volume (4 μL) sample cell allowed the measurement of cholesterol in serum with the addition of cholesterol oxidase. The biosensor exhibited a sensitivity to cholesterol of 2.1 μA/mM cm 2 ( r 2 = 0.97, n = 5) and was linear in the range of 0–15 mM.
- Published
- 2015
23. Laccase biosensor based on screen-printed electrode modified with thionine-carbon black nanocomposite, for Bisphenol A detection
- Author
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Fabiana Arduini, D. Di Tuoro, Marcella Cammarota, Maria Lepore, Marianna Portaccio, Danila Moscone, Damiano Gustavo Mita, Portaccio, Marianna Bianca Emanuela, Di Tuoro, D., Arduini, F., Moscone, D., Cammarota, M., Mita, D. G., and Lepore, Maria
- Subjects
Bisphenol A ,Nanocomposite ,Chemistry ,General Chemical Engineering ,Laccase ,Nanotechnology ,Carbon black ,Bisphenol A, Laccase, Carbon black, Thionine, Screen-printed electrode ,Thionine ,chemistry.chemical_compound ,Adsorption ,Screen-printed electrode ,Electrode ,Electrochemistry ,Settore CHIM/01 - Chimica Analitica ,Cyclic voltammetry ,Biosensor ,Nuclear chemistry - Abstract
The relevance of Bisphenol A (BPA) in human health is well-known. For this reason we designed and developed a biosensor based on a bionanocomposite (laccase–thionine–carbon black)-modified screen-printed electrode. Thionine, a commercially available dye, was used as electrochemical mediator coupled with a nanostructured carbon black. By means of cyclic voltammetry, the interaction of thionine adsorbed on modified screen printed electrode with laccase/BPA reaction products has been studied. In addition, the immobilization of laccase by physical adsorption on the surface of thionine–carbon black modified screen printed electrodes was investigated. The response of the biosensor has been optimized in terms of enzyme loading, pH and applied potential reaching a linear concentration range of 0.5–50 μM, a sensitivity of 5.0 ± 0.1 nA/μM and a limit-of-detection (LOD) of 0.2 μM. The developed biosensor has been also challenged in tomato juice samples contained in metallic cans where release of BPA due to the epoxy resin coating can be assumed. A satisfactory recovery value comprised between 92% and 120% was obtained.
- Published
- 2013
24. An acetylcholinesterase biosensor for determination of low concentrations of Paraoxon and Dichlorvos
- Author
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Di Tuoro, D, Portaccio, M, Lepore, M, Vitiello, A, Arduini, F, MOSCONE DINIA, D, Bencivenga, U, Mita, Dg, DI TUORO, D, Portaccio, Marianna Bianca Emanuela, Lepore, Maria, Arduini, F, Moscone, D, Bencivenga, U, and Mita, D. G.
- Subjects
Insecticides ,concentration ,dichlorvo ,tmb ,Bioengineering ,Biosensing Techniques ,base biosensor ,biosensor ,chemistry.chemical_compound ,Limit of Detection ,Bromide ,Dichlorvos ,pam ,medicine ,ache ,acetylthiocholine ,Animals ,Humans ,Organic chemistry ,Settore CHIM/01 - Chimica Analitica ,ppb ,Molecular Biology ,pesticide ,Chromatography ,Paraoxon ,paraoxon ,acetylcholinesterase ,paste ,current intensity ,electrode ,Substrate (chemistry) ,Electrochemical Techniques ,General Medicine ,Enzymes, Immobilized ,Oxime ,Acetylcholinesterase ,chemistry ,Acetylthiocholine ,Cholinesterase Inhibitors ,Oxidation-Reduction ,Biosensor ,Biotechnology ,medicine.drug - Abstract
The characterization of an economic and ease-to-use carbon paste acetylcholinesterase (AChE) based biosensor to determine the concentration of pesticides Paraoxon and Dichlorvos is discussed. AChE hydrolyses acetylthiocholine (ATCh) in thiocoline (TC) and acetic acid (AA). When AChE is immobilized into a paste carbon working electrode kept at +410 mV vs. Ag/AgCl electrode, the enzyme reaction rate using acetylthiocholine chloride (ATCl) as substrate is monitored as a current intensity. Because Paraoxon and Dichlorvos inhibit the AChE reaction, the decrease of the current intensity, at fixed ATCl concentration, is a measure of their concentration. Linear calibration curves for Paraoxon and Dichlorvos determination have been obtained. The detection limits resulted to be 0.86 ppb and 4.2 ppb for Paraoxon and Dichlorvos, respectively, while the extension of the linear range was up 23 ppb for the former pesticide and up to 33 ppb for the latter. Because the inhibited enzyme can be reactivated when immediately treated with an oxime, the biosensor reactivation has been studied when 1,1'-trimethylene bis 4-formylpyridinium bromide dioxime (TMB-4) and pyridine 2-aldoxime methiodide (2-PAM) were used. TMB-4 resulted more effective. The comparison with the behavior of similar AChE based biosensors is also presented.
- Published
- 2011
25. Enzymatic determination of BPA by means of tyrosinase immobilized on different carbon carriers
- Author
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A. Attanasio, Umberto Bencivenga, Danila Moscone, Aziz Amine, Nadia Diano, V. Grano, Silvia Rossi, Damiano Gustavo Mita, Fabiana Arduini, Mita, Dg, Attanasio, A, Arduini, F, Diano, Nadia, Grano, V, Bencivenga, U, Rossi, S, Amine, A, and Moscone, D.
- Subjects
Immobilized enzyme ,Tyrosinase ,Biomedical Engineering ,Biophysics ,Enzyme electrode ,chemistry.chemical_element ,Carbon nanotube ,Biosensing Techniques ,Hexadecane ,Sensitivity and Specificity ,law.invention ,chemistry.chemical_compound ,Phenols ,law ,Electrochemistry ,Organic chemistry ,Settore CHIM/01 - Chimica Analitica ,Benzhydryl Compounds ,Chromatography ,Chemistry ,Monophenol Monooxygenase ,Reproducibility of Results ,General Medicine ,Equipment Design ,Enzymes, Immobilized ,Amperometry ,Carbon ,Equipment Failure Analysis ,Biosensor ,Biotechnology ,Environmental Monitoring - Abstract
Different tyrosinase carbon paste modified electrodes to determine bisphenol A (BPA) concentration in aqueous solutions have been constructed. Variables examined were in the carbon paste composition and in particular: (i) the immobilized enzyme amount; (ii) the carbon type (powder, single or multi-walled nanotubes); (iii) the nature of the pasting oil (mineral oil, hexadecane and dodecane). For each biosensor type the amperometric response was evaluated with reference to the linear range and sensitivity. Constant reference has been made to the amperometric signals obtained, under the same experimental conditions, towards the catechol, a specific phenolic substrate for tyrosinase. The most efficient biosensors were those constructed by using the following composition for the carbon paste: 10% of tyrosinase, 45% of single wall carbon nanotubes (SWCN) and 45% of mineral oil. This biosensor formulation displayed the following electrochemical characteristics: a sensitivity equal to 138 μA/mM, LOD of 0.02 μM (based on three times the S/N ratio), linear range of 0.1–12 μM and response time of 6 min. This experimental work represents a first attempt at construction of a new carbon nanotube-tyrosinase based biosensor able to determine the concentration of BPA, one of the most ubiquitous and hazardous endocrine disruptors which can pollute the drinking and surface water, as well as many products of the food chain.
- Published
- 2006
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