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Your search keyword '"orientated antibody immobilization"' showing total 2 results
Start Over Descriptor "orientated antibody immobilization" Topic biosensing techniques
2 results on '"orientated antibody immobilization"'

1. Effective Construction of a High-Capacity Boronic Acid Layer on a Quartz Crystal Microbalance Chip for High-Density Antibody Immobilization.

Author

Lin PH, Huang SC, Chen KP, Li BR, and Li YK

Subjects
Adsorption, Aniline Compounds chemistry, Antibodies, Immobilized immunology, Electrodes, Gold chemistry, Humans, Antibodies, Immobilized chemistry, Biosensing Techniques, Boronic Acids chemistry, Quartz Crystal Microbalance Techniques
Abstract

Boronic acids (BAs) provide strong potential in orientation immobilization of antibody and the modification method is crucial for efficiency optimization. A highly effective method has been developed for rapid antibody immobilization on gold electrodes through the electrodeposition of a BA⁻containing linker in this study. Aniline-based BA forms a condense layer while antibody could automatically immobilize on the surface of the electrode. Compare to traditional self-assembled monolayer method, the electrodeposition process dramatically reduces the modification time from days to seconds. It also enhances the immobilized efficiency from 95 to 408 (ng/cm²) with a strong preference being exhibited for shorter aniline-based linkers.

Published
2018
Full Text
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2. Effective Construction of a High-Capacity Boronic Acid Layer on a Quartz Crystal Microbalance Chip for High-Density Antibody Immobilization

Author

Bor-Ran Li, Sheng Cih Huang, Kuang Po Chen, Yaw-Kuen Li, and Pei Heng Lin

Subjects
Materials science, boronic acid, Biosensing Techniques, 02 engineering and technology, lcsh:Chemical technology, 010402 general chemistry, 01 natural sciences, Biochemistry, Analytical Chemistry, quartz crystal microbalance, chemistry.chemical_compound, Aniline, Monolayer, Humans, lcsh:TP1-1185, Electrical and Electronic Engineering, Electrodes, orientated antibody immobilization, Instrumentation, Aniline Compounds, Communication, Quartz crystal microbalance, 021001 nanoscience & nanotechnology, Chip, Boronic Acids, Atomic and Molecular Physics, and Optics, 0104 chemical sciences, Chemical engineering, chemistry, Electrode, Quartz Crystal Microbalance Techniques, electrodeposition, Adsorption, Gold, 0210 nano-technology, Antibodies, Immobilized, Linker, Layer (electronics), Boronic acid
Abstract

Boronic acids (BAs) provide strong potential in orientation immobilization of antibody and the modification method is crucial for efficiency optimization. A highly effective method has been developed for rapid antibody immobilization on gold electrodes through the electrodeposition of a BA–containing linker in this study. Aniline-based BA forms a condense layer while antibody could automatically immobilize on the surface of the electrode. Compare to traditional self-assembled monolayer method, the electrodeposition process dramatically reduces the modification time from days to seconds. It also enhances the immobilized efficiency from 95 to 408 (ng/cm2) with a strong preference being exhibited for shorter aniline-based linkers.

Published
2018
Full Text
View/download PDF

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