1. A sticky-end probe biosensor for homogeneous detection of transcription factor binding activity.
- Author
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Mendes JPP, Zhu N, and Wong PK
- Subjects
- Fluorescence Resonance Energy Transfer methods, Transcription Factors, DNA, Biosensing Techniques methods
- Abstract
Transcription factors are essential regulators of various physiological and pathological processes. However, detecting transcription factor-DNA binding activities is often time-consuming and labor-intensive. Homogeneous biosensors that are compatible with mix-and-measure protocols have the potential to simplify the workflow for therapeutic screening and disease diagnostics. In this study, we apply a combined computational-experimental approach to investigate the design of a sticky-end probe biosensor, where the transcription factor-DNA complex stabilizes the fluorescence resonance energy transfer signal of the donor-acceptor pair. We design a sticky-end biosensor for the SOX9 transcription factor based on the consensus sequence and characterize its sensing performance. A systems biology model is also developed to investigate the reaction kinetics and optimize the operating conditions. Taken together, our study provides a conceptual framework for the design and optimization of sticky-end probe biosensors for homogeneous detection of transcription factor-DNA binding activity., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Pak Kin Wong reports financial support was provided by NSF., (Copyright © 2023. Published by Elsevier Inc.)
- Published
- 2023
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