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Copyright of Revista CIATEC-UPF is the property of Revista CIATEC-UPF and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The contamination of soils by oily compounds has several environmental impacts, which can be reversed through bioremediation, using biosurfactants as auxiliaries in the biodegradation process. In this study, we aimed to perform ex situ bioremediation of biodiesel-contaminated soil using biosurfactants produced by Bacillus methylotrophicus. A crude biosurfactant was produced in a whey-based culture medium supplemented with nutrients and was later added to biodiesel-contaminated clayey soil. The produced lipopeptide biosurfactant could reduce the surface tension of the fermentation broth to 30.2 mN/m. An increase in the microbial population was observed in the contaminated soil; this finding can be corroborated by the finding of increased CO2 release over days of bioremediation. Compared with natural attenuation, the addition of a lower concentration of the biosurfactant (0.5% w/w in relation to the mass of diesel oil) to the soil increased biodiesel removal by about 16% after 90 days. The added biosurfactant did not affect the retention of the contaminant in the soil, which is an important factor to be considered when applying in situ bioremediation technologies. [ABSTRACT FROM AUTHOR]

Copyright of Revista CIATEC-UPF is the property of Revista CIATEC-UPF and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Bacteria immobilization on biochar is a promising approach to achieve high concentration and stability of microbial cells for several applications. The present review addressed the techniques utilized for bacteria immobilization on biochar, discussing the mechanisms involved in this process, as well as the further utilization in bioremediation and agriculture. This article presents three immobilization techniques, which vary according to their procedures and conditions, including cell growth, adsorption, and adaptation. The mechanisms for cell immobilization are primarily adsorption and biofilm formation on biochar. The favorable characteristics of biochar immobilization depend on the pyrolysis methods, raw materials, and properties of biochar, such as surface area, pore size, pH, zeta potential, hydrophobicity, functional groups, and nutrients. Scanning electron microscope (SEM) and colony forming unit (CFU) are the analyses commonly carried out to verify the efficiency of bacteria immobilization. The benefits of applying biochar-immobilized bacteria include soil decontamination and quality improvement, which can improve plant growth and crop yield. Therefore, this emerging technology represents a promising solution for environmental and agricultural purposes. However, it is important to evaluate the potential adverse impacts on native microbiota by introducing exogenous microorganisms. [Display omitted] • Adsorption concepts explains the mechanisms of bacteria immobilization on biochar. • Biochars pyrolyzed at higher temperatures (> 500 ºC) are preferred for bacteria immobilization. • EPS helps to immobilize bacteria on biochar, reducing the stress caused by contaminants during bioremediation. • The microorganisms able to grow in the presence of contaminants are promising for immobilization. [ABSTRACT FROM AUTHOR]