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Over the past century, the demand for petroleum products has increased rapidly, leading to higher oil extraction, processing and transportation, which result in numerous oil spills in coastal-marine environments. As the spilled oil can negatively affect the coastal-marine ecosystems, its transport and fates captured a significant interest of the scientific community and regulatory agencies. Typically, the environment has natural mechanisms (e.g., photooxidation, biodegradation, evaporation) to weather/degrade and remove the spilled oil from the environment. Among various oil weathering mechanisms, biodegradation by naturally occurring bacterial populations removes a majority of spilled oil, thus the focus on bioremediation has increased significantly. Helping in the marginal recognition of this promising technique for oil-spill degradation, this paper reviews recently published articles that will help broaden the understanding of the factors affecting biodegradation of spilled oil in coastal-marine environments. The goal of this review is to examine the effects of various environmental variables that contribute to oil degradation in the coastal-marine environments, as well as the factors that influence these processes. Physico-chemical parameters such as temperature, oxygen level, pressure, shoreline energy, salinity, and pH are taken into account. In general, increase in temperature, exposure to sunlight (photooxidation), dissolved oxygen (DO), nutrients (nitrogen, phosphorous and potassium), shoreline energy (physical advection—waves) and diverse hydrocarbon-degrading microorganisms consortium were found to increase spilled oil degradation in marine environments. In contrast, higher initial oil concentration and seawater pressure can lower oil degradation rates. There is limited information on the influences of seawater pH and salinity on oil degradation, thus warranting additional research. This comprehensive review can be used as a guide for bioremediation modeling and mitigating future oil spill pollution in the marine environment by utilizing the bacteria adapted to certain conditions. [ABSTRACT FROM AUTHOR]

Microbial consortia-mediated biodegradation of 1,4-dioxane (1,4-D), an emerging water contaminant, is always a superior choice over axenic cultures. Thus, better understanding of the functions of coexisting microbes and their interspecies interactions within the consortia is crucial for predicting biodegradation efficiency and designing efficient 1,4-D-degrading microbial consortia. This study evaluated how microbial community compositions and interspecies interactions govern the microbial consortia-mediated 1,4-D biodegradation by investigating the biodegradability and microbial community dynamics of both enriched (N112) and synthetic (SCDs and SCDNs) microbial consortia in the absence or presence of additional organic compound (AOC). In the absence of AOC, N112 exhibited 100% 1,4-D biodegradation efficiency at a rate of 12.5 mg/L/d, whereas the co-occurrence of AOC resulted in substrate-dependent biodegradation inhibition and thereby reduced the biodegradation efficiency and activity (2.0–10.0 mg/L/d). The coexistence and negative influence of certain low-abundant non-degraders on both 1,4-D-degraders and key non-degraders in N112 was identified as the prime cause behind such biodegradation inhibition. Comparing with N112, SCDN-1 composed of 1,4-D-degraders and key non-degraders significantly improved the 1,4-D biodegradation efficiency in the presence of AOC, confirming the absence of negative influence of low-abundant non-degraders and cooperative interactions between 1,4-D-degraders and key non-degraders in SCDN-1. On the contrary, both two-species and three-species SCDs comprised of only 1,4-D-degraders resulted in lower 1,4-D biodegradation efficiency as compared to SCDN-1 under all treatment conditions, while max. 91% 1,4-D biodegradation occurred by SCDs in the absence of AOC. These results were attributed to the negative interaction among 1,4-D-degraders and the absence of complementary roles of key non-degraders in SCDs. The findings improve our understanding of how interspecies interactions can regulate the intrinsic abilities and functions of coexisting microbes during biodegradation in complex environments and provide valuable guidelines for designing highly efficient and robust microbial consortia for practical bioremediation of 1,4-D like emerging organic contaminants. [Display omitted] • 1,4-dioxane degradation efficiency of enriched and synthetic consortia were studied. • Additional substrate-dependent biodegradation efficiency was observed. • Biodegradation efficacy was governed by interspecies interactions within consortium. • A synthetic consortium having superior biodegradation efficiency was constructed. • Not all but key non-degraders were the prime contributors for efficient biodegradation. [ABSTRACT FROM AUTHOR]

Bioremediation is one of the promising environment-friendly approaches to eliminate oil contamination. However, heavy oil is known to degrade slowly due to its hydrophobicity. Therefore, microorganisms capable of producing biosurfactants are gaining substantial interest because of their potential to alter hydrocarbon properties and thereby speed up the degradation process. In this study, six bacterial consortia were obtained from the oil-spilled beach areas in Miyagi, Japan, and all of which exhibited high potential in degrading heavy oil measured by gas chromatography with flame ionization detector (GC-FID). The polymerase chain reaction—denaturing gradient gel electrophoresis (PCR-DGGE) and next-generation sequencing (NGS) revealed that the diverse microbial community in each consortium changed with subculture and became stable with a few effective microorganisms after 15 generations. The total petroleum hydrocarbons (TPH) degradation ability of the consortia obtained from a former gas station (C1: 81%) and oil refinery company (C6: 79%) was higher than that of the consortia obtained from wastewater treatment plant (WWTP) (C3: 67%, and C5: 73%), indicating that bacteria present in C1 and C6 were historically exposed to petroleum hydrocarbons. Moreover, it was intriguing that the consortium C4, also obtained from WWTP, exhibited high TPH degradation ability (77%). The NGS results revealed that two bacteria, Achromobacter sp. and Ochrobactrum sp., occupied more than 99% of the consortium C4, while no Pseudomonas sp. was found in C4, though this bacterium was observed in other consortia and is also known to be a potential candidate for TPH degradation as reported by previous studies. In addition, the consortium C4 showed high biosurfactant-producing ability among the studied consortia. To date, no study has reported the TPH degradation by the combination of Achromobacter sp. and Ochrobactrum sp.; therefore, the consortium C4 provided an excellent opportunity to study the interaction of and biosurfactant production by these two bacteria during TPH degradation. [ABSTRACT FROM AUTHOR]

Carbon tetrachloride (CT) is a recalcitrant and high priority pollutant known for its toxicity, environmental prevalence, and inhibitory activities. Although much is known about anaerobic CT biodegradation, microbial degradation of CT under aerobic conditions has not yet been reported. This study reports for the first time the enrichment of a stable aerobic CT-degrading bacterial consortium, from a CT-contaminated groundwater sample, capable of co-metabolically degrading 30 μM of CT within a week. A Pseudomonas strain (designated as Stari2) that is the predominant bacterium in this consortium was isolated, and further characterization showed that this bacterium can tolerate and co-metabolically degrade up to 5 mM of CT under aerobic conditions in the presence of different carbon/energy sources. The CT biodegradation profiles of strain Stari2 and the consortium were found to be identical, while no significant positive correlation between strain Stari2 and other bacteria was observed in the consortium during the period of higher CT biodegradation. These results confirmed that the isolated Pseudomonas strain Stari2 is the key player in the consortium catalyzing the biodegradation of CT. No chloroform (CF) or other chlorinated compound was detected during the cometabolism of CT. The whole genome sequencing of strain Stari2 showed that it is a novel Pseudomonas species. The findings demonstrated that biodegradation of CT under aerobic conditions is feasible, and the isolated CT-degrader Pseudomonas sp. strain Stari2 has a great potential for in-situ bioremediation of CT-contaminated environments. [Display omitted] • First report of carbon tetrachloride (CT) biodegradation in aerobic conditions. • An aerobic CT degrading bacterial consortium was enriched. • Pseudomonas sp. strain Stari2 was isolated as the key CT-degrader. • Strain Stari2 can tolerate and degrade up to 5 mM of CT cometabolically. [ABSTRACT FROM AUTHOR]

A dechlorinating consortium (designated as TES-1 culture) able to convert trichloroethene (TCE) to ethene was established from TCE-contaminated groundwater. This culture had the ability of complete dechlorination of TCE within about one month. From the clone library analysis of 16S rRNA gene, this culture was mainly composed of fermentation bacteria, such as Clostridium spp., and Desulfitobacterium spp. known as facultative dechlorinator. PCR using specific primers for Dehalococcoides spp. and the dehalogenase genes confirmed that the culture contained the Dehalococcoides spp. 16S rRNA gene and three dehalogenase genes, tceA, vcrA and bvcA. Dechlorination experiments using cis-dichloroethene (cis-DCE) at concentrations of 37-146 μM, revealed that the gene copy numbers of tceA, vcrA, and bvcA increased up to 107 copy/mL, indicating that Dehalococcoides spp. containing these three dehalogenase genes were involved in cis-DCE dechlorination. However, in the culture to which 292 μM of cis-DCE was added, only the tceA gene and the Dehalococcoides spp. 16S rRNA gene increased up to 107 copy/mL. The culture containing 292 μM of cis-DCE also exhibited about one tenth slower ethene production rate compared to the other cultures. [ABSTRACT FROM AUTHOR]

Abstract: Numerous studies on the biodegradation of petroleum products using total petroleum hydrocarbons (TPH) have been carried out; however, the biodegradation of equivalent carbon number (EC) based hydrocarbon fractions in mineral oil has attracted little attention. This study investigated the ability of a microbial consortium to degrade the EC fractions in kerosene, which was used as a representative mineral oil. Based on the cloning and sequencing of the 16S rRNA gene, the microbial community was predominantly identified as Betaproteobacteria of the genera Achromobacter, Alcaligenes, and Cupriavidus. Degradation experiments in sealed 120-ml vials containing 1% (w v−1) kerosene revealed that aromatic fractions were degraded faster than aliphatic fractions. Aromatic fractions EC >7–8 and EC >8–10 were completely degraded after three days while aliphatic fractions EC >6–8 and EC >8–10 were only partially degraded. The aromatic EC >10–12 fraction was the third most degraded, and the aliphatic EC >10–12 and EC >12–16 fractions were the least degraded fractions. The first-order rate constants for the aromatic fractions ranged from 0.12 d−1 to 0.51 d−1 and from 0.06 d−1 to 0.32 d−1 for the aliphatic fractions. The microbial consortium preferentially utilized aromatic fractions, which are more toxic than aliphatic fractions. This finding is useful when considering risk-based bioremediation: A microbial community could potentially degrade the more toxic aromatic hydrocarbon components in petroleum-contaminated environments. [ABSTRACT FROM AUTHOR]