1. Sialylation of N-Carbohydrate Chains of Glycoproteins with Trans-Sialidase from Trypanosoma cruzi
- Author
-
N. V. Bovin, V. V. Nasonov, V. S. Zueva, L. S. Zhigis, S. D. Shiyan, and N. C. Coi
- Subjects
chemistry.chemical_classification ,Glycan ,biology ,Glycoconjugate ,Organic Chemistry ,biology.organism_classification ,Biochemistry ,Trans-sialidase ,carbohydrates (lipids) ,Carbohydrate chains ,chemistry ,Pi ,biology.protein ,Bioorganic chemistry ,Glycoprotein ,Trypanosoma cruzi - Abstract
α2,3-Sialylation of the lactosamine type N-glycans with trans-sialidase from Trypanosomacruzi is reported. Trans-sialidase (160 kDa, pI 5.35–5.65) and its catalytic fragment (70 kDa, pI 6.0–6.3) were isolated from T. сruzi cells and immobilized on ConA-Sepharose. The resulting preparation retained its activity for several months and was repeatedly used for obtaining mono-, di-, tri-, and tetrasialylated 7-amino-4-metylcoumarin-labeled oligosaccharides with various numbers of antennas and for α2,3-sialylation of glycans within glycoproteins and neoglycoconjugates.
- Published
- 2004