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Graphical abstract Schematic illustration of graphene cellulose (G-C) paper fabrication, application for 3D multilayered laminate cell-laden constructs, and ensuing "origami-inspired" sculpting for 3D tissue engineering and regeneration. Highlights • Efficient method for graphene-cellulose (G-C) paper fabrication. • G-C paper is electroconductive, mechanically robust, flexible and biocompatible. • G-C paper can be employed for 2D or 3D stem cell support and differentiation. • G-C papers with cell-laden alginate can be stacked as 3D multilayered constructs. • Multilayered cell-laden constructs can be further configured by folding or rolling. Abstract Graphene-based materials represent advanced platforms for tissue engineering and implantable medical devices. From a clinical standpoint, it is essential that these materials are produced using non-toxic and non-hazardous methods, and have predictable properties and reliable performance under variable physiological conditions; especially when used with a cellular component. Here we describe such a biomaterial, namely smart graphene-cellulose (G-C) paper, and its suitability for traditional planar two-dimensional (2D) or three-dimensional (3D) human cell support, verified by adipose-derived stem cell (ADSC) culture and osteogenic differentiation. G-C paper is prepared using commercially available cellulose tissue paper as a substrate that is coated by immersion-deposition with graphene oxide (GO) followed by reduction to reduced graphene oxide (RGO) without the use of toxic organic solvents. The fabrication process is amenable to large scale production and the resultant papers have low electrical resistivity (up to ∼300 Ω/sq). Importantly, G-C papers can be configured to 3D constructs by lamination with alginate and further modified by folding and rolling for 3D "origami-inspired" cell-laden structures. [ABSTRACT FROM AUTHOR]

Abstract The research progress in the centrifugal microfluidic platform provides great opportunities for simple and effective analytical measurements in a variety of areas including biomedical engineering. In this study, we propose an optical reader that can measure the transmittance in a very sensitive and rapid manner on a hybrid paper-polymer centrifugal disc platform. This device enables real-time monitoring of multiple samples by measuring the absorbance of the light transmitted through the paper integrated on the disc between the light emitting diode (LED) and the photodiode (PD) regardless of the ambient light condition. To confirm its efficiency, we analyzed one of the blood's important indicators, glucose in a successful manner within 10 s without any additional complex image analysis. In addition, we discussed the results by comparing with the reflectance-based methods and with those of the previously reported studies by introducing a figure of merit to evaluate the performance of the assay. Highlights • Optical reader was developed for hybrid paper-polymer centrifugal disc platform. • This device enables real-time detection by measuring light transmission through the paper integrated on the disc between LED and photodiode. • As proof of concept, enzymatic colorimetric glucose detection was successfully performed. • Micromolar detection was achieved using the present technique within 10 s without any additional complex image analysis. [ABSTRACT FROM AUTHOR]